Abstract: A method of producing transformed Gramineae comprising making a wound in a seedling in an area of the seedling containing rapidly dividing cells and in ovulating the wound with vir+ Agrobacterium tumefaciens. Also, this same method wherein the vir+ A. tumefaciens contains a vector comprising genetically-engineered T-DNA. There are further provided a transformed pollen grain of a Gramineae, a pollen grain of a Gramineae produced by a plant grown from a seedling infected with vir+ A. tumefaciens, a pollen grain of a Gramineae produced by a plant grown from a seedling infected with vir+ A. tumefaciens containing a vector comprising genetically-engineered T-DNA, a pollen grain of a Gramineae whose cells contain a segment of T-DNA, and Gramineae derived from each of these pollen grains.

Abstract: A method of producing transformed Gramineae comprising making a wound in a seedling in an area of the seedling containing rapidly dividing cells and inoculating the wound with vir.sup.+ Agrobacterium tumefaciens. Also, this same method wherein the vir.sup.+ A. tumefaciens contains a vector comprising genetically-engineered T-DNA. There are further provided a transformed pollen grain of a Gramineae, a pollen grain of a Gramineae produced by a plant grown from a seedling infected with vir.sup.+ A. tumefaciens, a pollen grain of a Gramineae produced by a plant grown from a seedling infected with vir.sup.+ A. tumefaciens containing a vector comprising genetically-engineered T-DNA, a pollen grain of a Gramineae whose cells contain a segment of T-DNA, and Gramineae derived from each of these pollen grains. There are also provided a transformed Gramineae plant, a transformed Gramineae plant derived from a seedling infected with vir.sup.

Abstract: A non-tissue culture process using Agrobacterium-mediated vectors to produce transgenic plants from seeds of such plants as the common bean and soybean.

Abstract: A method of producing a transformed Gladiolus plant comprising: removing a piece of tissue from a corm; inoculating the tissue with vir.sup.+ Agrobacterium tumefaciens; incubating the inoculated tissue until a tumor forms; culturing at least a portion of the tumor in hormone-free medium until a cormel forms; and growing the cormel to produce the transformed plant. Also, methods of producing a transformed Gladiolus corm or seed comprising growing a transformed Gladiolus plant, prepared as just described, until the corm or seed is formed. Finally, transformed Gladiolus plants, corms and seeds.

Abstract: A method of producing transformed Gramineae comprising making a wound in a seedling in an area of the seedling containing rapidly dividing cells and inoculating the wound with vir.sup.+ Agrobacterium tumefaciens. Also, this same method wherein the vir.sup.+ A. tumefaciens contains a vector comprising genetically-engineered T-DNA. There are further provided a transformed pollen grain of a Gramineae, a pollen grain of a Gramineae produced by a plant grown from a seedling infected with vir.sup.+ A. tumefaciens, a pollen grain of a Gramineae produced by a plant grown from a seedling infected with vir.sup.+ A. tumefaciens containing a vector comprising genetically-engineered T-DNA, a pollen grain of a Gramineae whose cells contain a segment of T-DNA, and Gramineae derived from each of these pollen grains. There are also provided a transformed Gramineae plant, a transformed Gramineae plant derived from a seedling infected with vir.sup.

Abstract: A method of producing transformed corn comprising making a wound in a corn seedling in an area of the corn seedling containing rapidly dividing cells and inoculating the wound with vir.sup.+ Agrobacterium tumefaciens. Also, this same method wherein the vir.sup.+ A. tumefaciens contains a vector comprising genetically-engineered T-DNA. There are further provided a transformed corn pollen grain, a corn pollen grain produced by a plant grown from a seedling infected with vir.sup.+ A. tumefaciens, a corn pollen grain produced by a plant grown from a seedling infected with vir.sup..degree. A. tumefaciens containing a vector comprising genetrically-engineered T-DNA, a corn pollen grain whose cells contain a segment of T-DNA, and corn derived from each of these pollen grains. There are also provided a transformed corn plant, a transformed corn plant derived from a corn seedling infected with vir.sup.+ Agrobacterium tumefaciens, a transformed corn plant derived from a corn seedling infected with vir.sup.+ A.

Abstract: A non-tissue culture process using Agrobacterium-mediated vectors to produce transgenic plants from seeds of such plants as the common bean and soybean.