Abstract: An article to perform surface plasmon resonance imaging includes a light source to provide source light, the source light including a source optical profile, the source optical profile being selected to match a transmission profile at a surface plasmon resonance angle; an optical transformer configured to: receive the source light from the light source; and produce a transformed light comprising the source optical profile; and an optical modifier including: a back focal plane disposed at a first surface of the optical modifier and including the transmission profile; and an image focal plane disposed at a second surface of the optical modifier opposing the back focal plane, the optical modifier being configured to: magnify the transformed light; and produce magnified transformed light that includes the source optical profile.

Abstract: A kit includes a reference probe to react with a first sample; and an analyte probe to combine with a second sample, a marker and a spectral probe to react to form the analyte probe to combine with the second sample, the marker to react with the reference probe to form the analyte probe to combine with the second sample, or a combination thereof. A process for staining includes forming a reference composition by reacting a reference probe and a first sample to form a reference; and forming an analyte composition by combining an analyte probe and a second sample to form an analyte.

Abstract: An apparatus for the monitoring of a column chromatography separation process includes a segmented column with a seal positioned at the joint defined by the segments of the column. A connector is provided for connecting the segments of the column together. The apparatus further includes a sensor for monitoring an analyte in an eluant within a separation zone of the column. The sensor includes a mesh grid made of optical fibers or metal wires which is placed so as to extend through the separation zone of the column. The metal wires or optical fibers extend through the seal of the joint in the segmented column and connect to signal processing and data analysis equipment for purposes of monitoring the movements and concentration of an analyte in an eluant at various locations within the column. Certain segments of the optical fibers or metal wires which make up the mesh grid are coated so as to be desensitized and other segments are uncoated for sensing the analyte.

Abstract: A method of immunoanalysis combines immobilized immunochemistry with the technique of flow injection analysis, and employs microscopic spherical structures called liposomes, or lipid vesicles, as carriers of detectable reagents. Liposomes are modified on their surface with analytical reagents, and carry in their internal volume a very large number of fluorescent or electroactive molecules. Aspects of this embodiment of the invention include the chemistry for covalent immobilization of antibody fragments in a specified orientation, the use of liposomes in a flow injection analysis system, and the combination of automated sampling and analysis with reusable immunoreactants. Another aspect of the invention involves the non-covalent binding of liposomes to a receptor for use in a homogeneous assay. In another aspect of the invention the intensity of scattered light is quantitated as a measure of liposome aggregation in response to a concentration-dependent immunospecific reaction.