Abstract: A method of determining exposure of an individual to a macrocyclic trichothecene comprises isolating a sample of at least a portion of a naturally occurring protein from an individual, and detecting a reaction of the sample with a macrocyclic trichothecene. The macrocyclic trichothecene may be a product of Stachybotrys, such as satratoxin G. The sample may be a blood protein or a metabolic product of a satratoxin adduct found in urine. The interaction of the protein and the macrocyclic trichothecene may be detected by any one or more of a number of methods. The sample may be a proteinase product of a naturally occurring protein. A kit for determining the exposure of an individual to a macrocyclic trichothecene comprises an antibody that reacts to a macrocyclic trichothecene covalently bound to an amino acid derived from a naturally occurring protein.