Abstract: The present invention relates to a biosynthetic route to intermediates of the QS-21 molecule which are triterpenes from a saponin extract of the tree Quillaja saponaria comprising a quillaic acid backbone, as well as routes to make the QS-21 molecule, enzymes involved, the products produced and uses of the product.

Abstract: The invention relates generally to materials and methods for biosynthesising quillaic acid in a host by expressing heterologous nucleotide sequences in the host each of which encodes a polypeptide which in combination have said QA biosynthesis activity. Example polypeptides include (i) a Beta-amyrin synthase; (ii) an enzyme capable of oxidising Beta-amyrin or an oxidised derivative thereof at the C-28 position to a carboxylic acid; (iii) an enzyme capable of oxidising Beta-amyrin or an oxidised derivative thereof at the C-16a position to an alcohol; and (iv) an enzyme capable of oxidising Beta-amyrin or an oxidised derivative thereof at the C-23 position to an aldehyde. Preferred nucleotide sequences are obtained from, or derived from, Q. saponaria.

Abstract: The present invention relates generally to genes and polypeptides which have utility in glycosylating quillaic acid in host cells, including enzymes capable of successive glycosylation at the C-3 position of quillaic acid. The invention further relates to systems, methods and products employing the same.

Abstract: This invention relates to isolated polynucleotides encoding a CYP51H. The invention also relates to the construction of recombinant DNA constructs comprising all or a portion of the isolated polynucleotide of the invention, in sense or antisense orientation, operably linked to at least one regulatory sequence.

Abstract: This invention relates to isolated polynucleotides encoding enzymes consisting of a carboxypeptidase-like protein, a methyltransferase and a glucosyltransferase, involved in the biosynthesis of ?-amyrin-derived triterpenes in plants and seeds. The invention also relates to the construction of recombinant DNA constructs comprising all or a portion of the isolated polynucleotides of the invention, in sense or antisense orientation, operably linked to at least one regulatory sequence.

Abstract: This invention relates to isolated polynucleotides encoding enzymes consisting of a carboxypeptidase-like protein, a methyltransferase and a glucosyltransferase, involved in the biosynthesis of ?-amyrin-derived triterpenes in plants and seeds. The invention also relates to the construction of recombinant DNA constructs comprising all or a portion of the isolated polynucleotides of the invention, in sense or antisense orientation, operably linked to at least one regulatory sequence.

Abstract: This invention relates to isolated polynucleotides encoding enzymes consisting of a carboxypeptidase-like protein, a methyltransferase and a glucosyltransferase, involved in the biosynthesis of ?-amyrin-derived triterpenes in plants and seeds. The invention also relates to the construction of recombinant DNA constructs comprising all or a portion of the isolated polynucleotides of the invention, in sense or antisense orientation, operably linked to at least one regulatory sequence.

Abstract: This invention relates to isolated polynucleotides encoding enzymes consisting of a carboxypeptidase-like protein, a methyltransferase and a glucosyltransferase, involved in the biosynthesis of ?-amyrin-derived triterpenes in plants and seeds. The invention also relates to the construction of recombinant DNA constructs comprising all or a portion of the isolated polynucleotides of the invention, in sense or antisense orientation, operably linked to at least one regulatory sequence.

Abstract: This invention relates to isolated polynucleotides encoding a CYP51H. The invention also relates to the construction of recombinant DNA constructs comprising all or a portion of the isolated polynucleotide of the invention, in sense or antisense orientation, operably linked to at least one regulatory sequence.

Abstract: This invention relates to isolated polynucleotides encoding a CYP51H. The invention also relates to the construction of recombinant DNA constructs comprising all or a portion of the isolated polynucleotide of the invention, in sense or antisense orientation, operably linked to at least one regulatory sequence.

Abstract: This invention relates to isolated polynucleotides encoding a CYP51H. The invention also relates to the construction of recombinant DNA constructs comprising all or a portion of the isolated polynucleotide of the invention, in sense or antisense orientation, operably linked to at least one regulatory sequence.