Patents by Inventor Anne Tscheliessnig

Anne Tscheliessnig has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Production of recombinant proteins by autoproteolytic cleavage of a fusion protein
    Patent number: 8372959
    Abstract: Disclosed is a method for the production of a heterologous polypeptide of interest with a homogenous N-terminus, using a fusion polypeptide comprising the polypeptide of interest and N-terminally thereto a polypeptide exhibiting autoproteolytic function, said method comprises the steps of a) binding of the fusion polypeptide in a soluble, autoproteolytically inactive form by an affinity chromatography system, b) refolding of the fusion polypeptide, thereby activating the autoproteolytic function of the fusion polypeptide and causing cleavage of the heterologous polypeptide of interest, and c) subsequently eluting the heterologous polypeptide of interest, wherein the steps are conducted on one affinity chromatography system.
    Type: Grant
    Filed: January 11, 2012
    Date of Patent: February 12, 2013
    Assignees: Sandoz AG, Boehringer Ingelheim RCV GmbH & Co KG
    Inventors: Alois Jungbauer, Rainer Hahn, Anne Tscheliessnig, Waltraud Kaar
  • ORGANIC COMPOUNDS
    Publication number: 20120107869
    Abstract: Disclosed is a method for the production of a heterologous polypeptide of interest with a homogenous N-terminus, using a fusion polypeptide comprising the polypeptide of interest and N-terminally thereto a polypeptide exhibiting autoproteolytic function, said method comprising the steps of a) binding of the fusion polypeptide in a soluble, autoproteolytically inactive form by an affinity chromatography system, b) refolding of the fusion polypeptide, thereby activating the autoproteolytic function of the fusion polypeptide and causing cleavage of the heterologous polypeptide of interest, and c) subsequently eluting the heterologous polypeptide of interest, wherein said steps are conducted on one affinity chromatography system.
    Type: Application
    Filed: January 11, 2012
    Publication date: May 3, 2012
    Applicants: BOEHRINGER INGELHEIM RCV GmbH & Co KG, SANDOZ AG
    Inventors: Alois JUNGBAUER, Rainer Hahn, Anne Tscheliessnig, Waltraud Karr
  • Production of recombinant proteins by autoproteolytic cleavage of a fusion protein
    Patent number: 8163890
    Abstract: Disclosed is a method for the production of a heterologous polypeptide of interest with a homogenous N-terminus, using a fusion polypeptide comprising the polypeptide of interest and N-terminally thereto a polypeptide exhibiting autoproteolytic function, said method comprising the steps of a) binding of the fusion polypeptide in a soluble, autoproteolytically inactive form by an affinity chromatography system, b) refolding of the fusion polypeptide, thereby activating the autoproteolytic function of the fusion polypeptide and causing cleavage of the heterologous polypeptide of interest, and c) subsequently eluting the heterologous polypeptide of interest, wherein said steps are conducted on one affinity chromatography system.
    Type: Grant
    Filed: April 25, 2006
    Date of Patent: April 24, 2012
    Assignees: Sandoz AG, Boehringer Ingelheim RCV GmbH & Co KG
    Inventors: Alois Jungbauer, Rainer Hahn, Anne Tscheliessnig, Waltraud Kaar
  • Organic compounds
    Publication number: 20090203069
    Abstract: Disclosed is a method for the production of a heterologous polypeptide of interest with a homogenous N-terminus, using a fusion polypeptide comprising the polypeptide of interest and N-terminally thereto a polypeptide exhibiting autoproteolytic function, said method comprising the steps of a) binding of the fusion polypeptide in a soluble, autoproteolytically inactive form by an affinity chromatography system, b) refolding of the fusion polypeptide, thereby activating the autoproteolytic function of the fusion polypeptide and causing cleavage of the heterologous polypeptide of interest, and c) subsequently eluting the heterologous polypeptide of interest, wherein said steps are conducted on one affinity chromatography system.
    Type: Application
    Filed: April 25, 2006
    Publication date: August 13, 2009
    Applicants: BOEHRINGER INGELHEIM AUSTRIA GMBH, SANDOZ AG
    Inventors: Alois Jungbauer, Rainer Hahn, Anne Tscheliessnig, Waltraud Kaar