Abstract: A sealed multi-handled bag is provided that includes a first handle at a first end and a second handle at a second end. In one embodiment, the handles are on substantially opposite ends of the bag. The sealed bag may be made from plastic and may contain loose materials, such as a salt. In some embodiments, one or more of the handles are formed from heat-sealed layers of bag material having a grip cut therein. A method for filling and sealing the multi-handled sealed bag includes filling a bag having a first closed end through a second open end, and sealing the second open end to form a handle. In one embodiment, sealing the second open end includes heat-sealing opposing layers of bag material together and cutting a handle through the layers. In another embodiment, the method includes folding-over the layers of bag material.

Abstract: The present invention relates generally to the fields of biochemistry, molecular biology, and virology. More particularly, it relates to the identification of GB virus B (GBV-B)/HCV chimeras. The invention involves nucleic acid constructs and compositions encoding GBV-B/HCV chimera, including at least part of a 5′ NTR derived from a HCV 5′ NTR. This construct, and chimeric versions of it, may be employed to study GBV-B and related hepatitis family members, such as hepatitis C virus. The invention thus includes methods of preparing GBV-B/HCV chimeric sequences, constructs, and viruses, as well as methods of employing these compositions.

Abstract: A method of forming a layer of oxide on a surface of a wafer is disclosed, in which the wafer surface is heated at a first temperature and a first pressure during a first period of time in a first ambient gas comprising nitrogen and oxygen species at a first concentration, and the wafer surface is heated at a second temperature and a second pressure during a second period of time in a second ambient gas comprising ozone and oxygen at a second concentration. The oxide structure formed thereby is also disclosed.

Abstract: The invention relates to an active principle of a vaccine, in which the characteristic epitope is incorporated in the capsid of a picornavirus, in particular of poliovirus, used as a vector. This active principle consists of a viable hybrid picornavirus containing an epitope characteristic of a vaccinating protein, and heterologous with respect to the proteins of this picornavirus, in place of one of its own immunogenic epitopes.