Abstract: Subject matter of the invention is a new method of detecting a substance with hydrolase activity in a sample, characterized in that the sample is brought into contact with an indicator enzyme, which is covalently bound to an insoluble carrier material and can be rendered soluble by the hydrolase activity, in that the cleaved off indicator is separated and in that its enzymatic activity is determined and test means for its implementation.

Abstract: The present invention provides a process for the determination of an enzyme from an isoenzyme mixture in a liquid sample by inhibition of the disturbing isoenzymes and determination of the non-inhibited enzyme, wherein the isoenzyme mixture is contacted with one or more substances which are able to inhibit the disturbing isoenzymes, the sample containing the inhibiting substance(s) is transferred to a small-pored reaction medium and the disturbing enzyme is there inhibited and the determination of the non-inhibited enzyme is carried out in the resulting liquid.The present invention also provides a test carrier for carrying out this process.

Abstract: The present invention provides a diagnostic agent for the enzymatic determination of cholesterol, wherein, besides the necessary enzymes, it contains a scleroprotein or a scleroprotein hydrolysate; said invention also provides a process for the production of a reagent film for the detection of cholesterol, as well as a test strip for the detection of cholesterol; and said invention is also concerned with the use of scleroproteins and scleroprotein hydrolysates for increasing the enzyme stability in diagnostic agents for the detection of cholesterol and for increasing the temperature-independence of the detection of cholesterol with diagnostic agents.

Abstract: The present invention provides a test carrier for the analytical determination of a component of a liquid sample, especially of a body fluid, with a carrier layer, which is partly covered by a liquid-absorbing layer which contains a sample application zone and a transport zone, and a reagent layer which is in contact with the liquid-absorbing layer, wherein the reagent layer in contact with the liquid-absorbing layer is so arranged that it does not overlap with the sample application zone and is full-facedly in contact with the liquid-absorbing layer.

Abstract: Test carrier for analyzing a sample fluid with several test layers which form a sample fluid transport path and contain a reagent system which reacts with the sample fluid to produce a detectable signal. The test carrier includes a reservoir layer of absorbent material, a detection layer arranged in the fluid transport path downstream the reservoir layer, in which a detectable signal is formed, and a separating layer arranged between the reservoir layer and the detection layer. The separating layer makes a two-step process possible. Fluid contact between the reservoir layer and the detection layer arises only with pressure loading of the layer assembly of the reservoir layer, separating layer and detection layer. A more uniform optical detection signal, and consequently better accuracy, can be achieved because the separating layer is made up of a hydrophilic material, wich is in the form of a lattice-shaped structure, in which the mean width of the lattice openings is more than 0.

Abstract: The present invention provides a test carrier for the analysis of a sample liquid and especially of a body fluid, having a porous test layer (8, 13) which contains a solid component (9, 17), wherein the solid component (9, 17) is coated with a protein which is insoluble in the sample liquid under the test conditions. A process for the production of this test layer is also disclosed, wherein the protein is dissolved in a solvent under conditions under which the solubility of the protein is sufficiently high in order to dissolve a certain minimum amount of the protein, the solid component of the test layer is contracted with the solution and the solubility is reduced to such an extent that the component is coated by the precipitating protein.

Abstract: The present invention provides a process for the determination of an analyte in a body fluid, in which there are used two binding components capable of specifically binding with one another, one of the binding components being enzyme-labelled and not carrier-fixed and the other binding component being carrier-fixed. The process contains a step in which the binding components are incubated with one another so that binding reaction takes place. The amount of enzyme-labelled binding component not bound to the carrier-fixed binding component is a measure of the concentration of the analyte which is determined by allowing the labelling enzyme to act upon a substrate producing a detection signal.

Abstract: The present invention provides a multi-layer test carrier (1) for the analytical determination of a component in a liquid sample, especially in a body fluid, having a first carrier layer (2) and a second carrier layer (10), at least one of which is transparent, a liquid-absorbing layer (3) being applied to the first carrier layer (2) and a reagent layer (11) containing at least one reagent being applied to the second carrier layer (10), the carrier layers being so arranged and fixed that the surfaces (3a, 11a) of the absorbing layer and of the reagent layer facing away from the carrier layer can be brought into contact with one another and can be pressed against one another, wherein the reagent layer (11) is soluble substantially without residue in the sample liquid and the take-up ability of the liquid-absorbing layer (3) for the sample liquid is so great that an amount of liquid absorbed therein suffices in order to dissolve the reagent layer free of residues so that substances contained in the reagent laye

Abstract: The present invention provides a test carrier (1) for the analytical determination of a component of a body fluid with a base layer (2) and at least two planar test layers which, in the initial state of the test carrier, before carrying out the determination, are separate from one another but can be brought into contact with one another by external manipulation, wherein a first test layer (8) and a second test layer (10) are arranged on the base layer essentially next to one another but separated in the initial state by a gap, a contact element (11, 17, 18, 20) being provided which consists of a capillary-active material which is so dimensioned that it can bridge the gap (12) and which is so mounted and arranged that, in a first position, it cannot contact at least one of the test layers (8, 10) but, by external pressure, it can be brought into a second position in which it contacts both test layers in such a manner that a liquid exchange between the test layers is possible.

Abstract: A test strip has a reagent-containing film layer coatingly embedded at least preponderantly on and into only one side of a carrier layer made of a multifilar fabric or fleece.

Abstract: The present invention provides a process for the production of reagent films in which an aqueous synthetic resin dispersion which contains the usual adjuvants and reagents is coated on to a solid substrate or on to a thin fabric in a thin layer and dried, wherein to the crude film mass used for the coating there are added 0.5 to 5% by weight of a scleroprotein hydrolysate with a molecular weight of from 5000 to 50,000 and 0.5 to 10% by weight of a higher alcohol containing 5 to 10 carbon atoms.

Abstract: A test strip has a reagent-containing film layer coating embedded at least preponderantly on and into only one side of a carrier layer made of a multifilar fabric or fleece.

Abstract: A test strip for analyzing a liquid sample has a carrier or handle, a slowly-absorbent layer fixed thereupon which slowly sucks up the liquid sample, a liquid impermeable layer fixer thereover, an absorbent reagent layer fixed thereover, and a mesh covering these, which mesh layer is fixed next to the slowly-absorbent layer on to the carrier. The mesh and layer portions of the test strip can thus be briefly immersed in the liquid sample with the carrier and withdrawn for observing the analyzing reaction of the reagent and liquid sample absorbed into the absorbent reagent layer through the mesh, the mesh serving to conduct excess liquid sample from the adjacent surface of the absorbent reagent layer to the still absorbent slowly-absorbent layer so that the excess liquid sample does not interfere with the observation of the reaction.

Abstract: Bis-(2,4-dinitrophenyl)-methylpyridinium compounds useful as a pH indicator or for the detection of ammonia having the formula: ##STR1## wherein one of R.sub.1 and R.sub.2 is a bis-(2,4-dinitrophenyl)-methyl radical and the other is a lower alkyl radical;R.sub.2 can also be hydrogen atom or trifluoromethyl;R.sub.3 and R.sub.4 are hydrogen or, together with the carbon atoms to which they are attached, represent a benzene ring; andX.sup..crclbar. is an appropriate acid anionor said compounds are betaines.

Abstract: Diagnostic device for the determination of ammonia having a handle, an ammonia indicator layer thereon and an alkaline buffer-containing reaction layer positioned above the indicator layer a distance of 10 to 200 microns by a spacer member. The reaction layer is readily separated from the indicator layer to reveal the latter at the end of the ammonia test.