Abstract: The present invention relates to a kit and a method to identify M. tuberculosis, which may include an approach relating to isolating chromosomal DNA from a biological sample, such as a culture, conducting a real time PCR to obtain an amplicon, digesting said amplicon separately with restriction enzymes NruI and BamHI to obtain a digestion product separating said digestion product into its constituents in a gel; and analyzing the restriction enzyme digestion pattern.