Patents by Inventor Anson Hatch
Anson Hatch has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 10988723Abstract: The present invention relates to modular assemblies and systems for conducting cell analyses. In particular embodiments, an assembly has micron-scale fluidic structures, thereby minimizing sample use, and possesses a modular architecture that facilitates disassembly of the apparatus. Thus, one such modular assembly facilitates on-chip cell growth and on-chip assays; and then, as needed, the assembly can be disassembled to provide direct access to the cells that were incubated and treated within the chip. Methods of making and using such assemblies, as well as systems thereof, are further described herein.Type: GrantFiled: September 22, 2016Date of Patent: April 27, 2021Assignee: National Technology & Engineering Solutions of Sandia, LLCInventors: Anson Hatch, Vinay Abhyankar, Chung-Yan Koh
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Patent number: 8163154Abstract: We report unique findings on the voltage dependence of protein exclusion from the pores of nanoporous polymer exclusion membranes. The pores are small enough that proteins are excluded from passage with low applied electric fields, but increasing the field enables proteins to pass through. The requisite field necessary for a change in exclusion is protein-specific with a correlation to protein size. The field-dependence of exclusion is important to consider for preconcentration applications. The ability to selectively gate proteins at exclusion membranes is also a promising means for manipulating and characterizing proteins. We show that field-gated exclusion can be used to selectively remove proteins from a mixture, or to selectively trap protein at one exclusion membrane in a series.Type: GrantFiled: July 18, 2007Date of Patent: April 24, 2012Assignee: Sandia CorporationInventors: Anson Hatch, Anup K. Singh
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Patent number: 7828948Abstract: Disclosed herein are methods and devices for preconcentrating and separating analytes such as proteins and polynucleotides in microchannels. As disclosed, at least one size-exclusion polymeric element is adjacent to processing area or an assay area in a microchannel which may be porous polymeric element. The size-exclusion polymeric element may be used to manipulate, e.g. concentrate, analytes in a sample prior to assaying in the assay area.Type: GrantFiled: September 29, 2006Date of Patent: November 9, 2010Assignee: Sandia CorporationInventors: Anson Hatch, Anup K. Singh, Amy E. Herr, Daniel J. Throckmorton
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Patent number: 7271007Abstract: Methods and apparatuses are provided for determining presence and concentration of analytes by exploiting molecular binding reactions and differential diffusion rates. Analyte particles and binding particles are allowed to diffuse toward each other, and slowing of the diffusion front is detected when they meet. From the position of the diffusion front, presence and concentration of analyte particles can be determined. One embodiment provides a competitive immunoassay in a microfluidic format. This diffusion immunoassay (DIA) relies on measuring the concentration of labeled antigen along one dimension of a microchannel after allowing it to diffuse for a short time into a region containing specific antibodies. A simple microfluidic device, the T-Sensor, was used to implement a DIA to measure the concentration of phenytoin, a small drug molecule. Concentrations of analyte over the range of 50 to 1600 nM can be measured in less than a minute.Type: GrantFiled: February 18, 2003Date of Patent: September 18, 2007Assignee: University of WashingtonInventors: Bernhard H Weigl, Paul Yager, Andrew Kamholz, Anson Hatch
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Patent number: 7141429Abstract: This invention provides methods for using liquid junction potentials to control the transport of charged particles in fluid streams that are in laminar flow within microfluidic channels. Applications of the methods of this invention include sample preconditioning (removal of interfering substances), electrophoretic separation (fractionation) of charged particles, enhanced or delayed mixing of charged particles across a fluid interface relative to diffusion only, focusing charged particles in a fluid stream in one or two dimensions, and concentration of charged reactants at a fluid interface.Type: GrantFiled: October 9, 2002Date of Patent: November 28, 2006Assignee: University of WashingtonInventors: Matthew S. Munson, Catherine R. Cabrera, Paul Yager, Anson Hatch, Andrew Kamholz
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Publication number: 20060196771Abstract: This invention provides methods for using liquid junction potentials to control the transport of charged particles in fluid streams that are in laminar flow within microfluidic channels. Applications of the methods of this invention include sample preconditioning (removal of interfering substances), electrophoretic separation (fractionation) of charged particles, enhanced or delayed mixing of charged particles across a fluid interface relative to diffusion only, focusing charged particles in a fluid stream in one or two dimensions, and concentration of charged reactants at a fluid interface.Type: ApplicationFiled: May 22, 2006Publication date: September 7, 2006Applicant: University of WashingtonInventors: Matthew Munson, Catherine Cabrera, Paul Yager, Anson Hatch, Andrew Kamholz
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Publication number: 20060115905Abstract: A diffusion immunoassay (DIA) for determining the presence and concentration of analyte particles by detecting the diffusion front. A hydrogel containing immobilized binding particles is placed in contact with a carrier fluid containing analyte particles, which analyte particles diffuse into the hydrogel and bind with the immobilized binding particles. A detection device detects the position of the diffusion front formed in the hydrogel to determine the presence and concentration of the analyte particles which have diffused into the hydrogel.Type: ApplicationFiled: June 23, 2005Publication date: June 1, 2006Applicant: University of WashingtonInventors: Anson Hatch, Paul Yager
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Publication number: 20030211507Abstract: A diffusion immunoassay (DIA) for determining the presence and concentration of analyte particles by detecting the diffusion front. A hydrogel containing immobilized binding particles is placed in contact with a carrier fluid containing analyte particles, which analyte particles diffuse into the hydrogel and bind with the immobilized binding particles. A detection device detects the position of the diffusion front formed in the hydrogel to determine the presence and concentration of the analyte particles which have diffused into the hydrogel.Type: ApplicationFiled: October 21, 2002Publication date: November 13, 2003Inventors: Anson Hatch, Paul Yager
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Publication number: 20030124619Abstract: Methods and apparatuses are provided for determining presence and concentration of analytes by exploiting molecular binding reactions and differential diffusion rates. Analyte particles and binding particles are allowed to diffuse toward each other, and slowing of the diffusion front is detected when they meet. From the position of the diffusion front, presence and concentration of analyte particles can be determined. One embodiment provides a competitive immunoassay in a microfluidic format. This diffusion immunoassay (DIA) relies on measuring the concentration of labeled antigen along one dimension of a microchannel after allowing it to diffuse for a short time into a region containing specific antibodies. A simple microfluidic device, the T-Sensor, was used to implement a DIA to measure the concentration of phenytoin, a small drug molecule. Concentrations of analyte over the range of 50 to 1600 nM can be measured in less than a minute.Type: ApplicationFiled: February 18, 2003Publication date: July 3, 2003Inventors: Bernhard H. Weigl, Paul Yager, Andrew Kamholz, Anson Hatch
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Publication number: 20030102214Abstract: This invention provides methods for using liquid junction potentials to control the transport of charged particles in fluid streams that are in laminar flow within microfluidic channels. Applications of the methods of this invention include sample preconditioning (removal of interfering substances), electrophoretic separation (fractionation) of charged particles, enhanced or delayed mixing of charged particles across a fluid interface relative to diffusion only, focusing charged particles in a fluid stream in one or two dimensions, and concentration of charged reactants at a fluid interface.Type: ApplicationFiled: October 9, 2002Publication date: June 5, 2003Inventors: Matthew S. Munson, Catherine R. Cabrera, Paul Yager, Anson Hatch, Andrew Kamholz
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Patent number: 6541213Abstract: Methods and apparatuses are provided for determining presence and concentration of analytes by exploiting molecular binding reactions and differential diffusion rates. Analyte particles and binding particles are allowed to diffuse toward each other, and slowing of the diffusion front is detected when they meet. From the position of the diffusion front, presence and concentration of analyte particles can be determined. One embodiment provides a competitive immunoassay in a microfluidic format. This diffusion immunoassay (DIA) relies on measuring the concentration of labeled antigen along one dimension of a microchannel after allowing it to diffuse for a short time into a region containing specific antibodies. A simple microfluidic device, the T-Sensor, was used to implement a DIA to measure the concentration of phenytoin, a small drug molecule. Concentrations of analyte over the range of 50 to 1600 nM can be measured in less than a minute.Type: GrantFiled: May 19, 2000Date of Patent: April 1, 2003Assignee: University of WashingtonInventors: Bernhard H. Weigl, Paul Yager, Andrew Kamholz, Anson Hatch
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Publication number: 20020090644Abstract: Methods and apparatuses are provided for determining presence and concentration of analytes by exploiting molecular binding reactions and differential diffusion rates. Analyte particles and binding particles are allowed to diffuse toward each other, and slowing of the diffusion front is detected when they meet. From the position of the diffusion front, presence and concentration of analyte particles can be determined. One embodiment provides a competitive immunoassay in a microfluidic format. This diffusion immunoassay (DIA) relies on measuring the concentration of labeled antigen along one dimension of a microchannel after allowing it to diffuse for a short time into a region containing specific antibodies. A simple microfluidic device, the T-Sensor, was used to implement a DIA to measure the concentration of phenytoin, a small drug molecule. Concentrations of analyte over the range of 50 to 1600 nM can be measured in less than a minute.Type: ApplicationFiled: February 14, 2000Publication date: July 11, 2002Inventors: Bernhard H. Weigl, Paul Yager, Andrew Kamholz, Anson Hatch
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Patent number: 6415821Abstract: Magnetically actuated fluid handling devices using magnetic fluid to move one or more fluids (gases or liquids or both) through microsized flow channels are provided. Fluid handling devices include micropumps and microvalves. Magnetically actuated slugs of magnetic fluid are moved within microchannels of a microfluidic device to facilitate valving and/or pumping of fluids and no separate pump is required. The magnets used to control fluid movement can be either individual magnets moved along the flow channels or one or more arrays of magnets whose elements can be individually controlled to hold or move a magnetic slug. Fluid handling devices include those having an array of electromagnets positioned along a flow channel which are turned on and off in a predetermined pattern to move magnetic fluid slugs in desired paths in the flow channel. The fluid handling devices of the present invention can handle gases and liquids simultaneously and thus can be made to be self-priming.Type: GrantFiled: December 15, 2000Date of Patent: July 9, 2002Assignee: University of WashingtonInventors: Andrew Kamholz, Anson Hatch, Karl Bohringer, Paul Yager
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Patent number: 6408884Abstract: Magnetically actuated fluid handling devices using magnetic fluid to move one or more fluids (gases or liquids or both) through microsized flow channels are provided. Fluid handling devices include micropumps and microvalves. Magnetically actuated slugs of magnetic fluid are moved within microchannels of a microfluidic device to facilitate valving and/or pumping of fluids and no separate pump is required. The magnets used to control fluid movement can be either individual magnets moved along the flow channels or one or more arrays of magnets whose elements can be individually controlled to hold or move a magnetic slug. Fluid handling devices include those having an array of electromagnets positioned along a flow channel which are turned on and off in a predetermined pattern to move magnetic fluid slugs in desired paths in the flow channel. The fluid handling devices of the present invention can handle gases and liquids simultaneously and thus can be made to be self-priming.Type: GrantFiled: December 15, 1999Date of Patent: June 25, 2002Assignee: University of WashingtonInventors: Andrew Kamholz, Anson Hatch, Karl Böhringer, Paul Yager, Berhard Weigl
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Publication number: 20020076716Abstract: The present invention generally relates to high density nucleic acid arrays and methods of synthesizing nucleic acid sequences on a solid surface. Specifically, the present invention contemplates the use of stabilized nucleic acid primer sequences immobilized on solid surfaces, and circular nucleic acid sequence templates combined with the use of isothermal rolling circle amplification to thereby increase nucleic acid sequence concentrations in a sample or on an array of nucleic acid sequences.Type: ApplicationFiled: June 21, 2001Publication date: June 20, 2002Applicant: Trustees of Boston UniversityInventors: Chandran R. Sabanayagam, Takeshi Sano, John Misasi, Anson Hatch, Charles Cantor
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Patent number: 6284497Abstract: The present invention generally relates to high density nucleic acid arrays and methods of synthesizing nucleic acid sequences on a solid surface. Specifically, the present invention contemplates the use of stabilized nucleic acid primer sequences immobilized on solid surfaces, and circular nucleic acid sequence templates combined with the use of isothermal rolling circle amplification to thereby increase nucleic acid sequence concentrations in a sample or on an array of nucleic acid sequences.Type: GrantFiled: April 8, 1999Date of Patent: September 4, 2001Assignee: Trustees of Boston UniversityInventors: Chandran R. Sabanayagam, Takeshi Sano, John Misasi, Anson Hatch, Charles Cantor
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Publication number: 20010017158Abstract: Magnetically actuated fluid handling devices using magnetic fluid to move one or more fluids (gases or liquids or both) through microsized flow channels are provided. Fluid handling devices include micropumps and microvalves. Magnetically actuated slugs of magnetic fluid are moved within microchannels of a microfluidic device to facilitate valving and/or pumping of fluids and no separate pump is required. The magnets used to control fluid movement can be either individual magnets moved along the flow channels or one or more arrays of magnets whose elements can be individually controlled to hold or move a magnetic slug. Fluid handling devices include those having an array of electromagnets positioned along a flow channel which are turned on and off in a predetermined pattern to move magnetic fluid slugs in desired path in the flow channel. The fluid handling devices of the present invention can handle gases and liquids simultaneously and thus can be made to be self-priming.Type: ApplicationFiled: December 15, 2000Publication date: August 30, 2001Inventors: Andrew Kamholz, Anson Hatch, Karl Bohringer, Paul Yager