Abstract: Blockage in sterilizers due to the solidification of spilled liquid agar downstream from the sterilization chamber is prevented by heating the drain stream exiting the sterilization chamber so that the temperature of the liquid agar stays above 45.degree.-50.degree. C. prior to discharge from the sterilization plumbing system.An improved modular plumbing system for sterilizers is provided, where the piping and valving requirements for a discrete operation performed by the sterilizer are segregated in a single manifold.

Abstract: A particularly useful method is disclosed for sterilizing micro-isolators in which a sipper bottle is contained. In the present invention, sterilization is effected by using a microprocessor control to regulate the pressure within a sterilization chamber in response to temperature conditions. Pressure within the chamber is regulated so that as temperature is raised and lowered, the boiling point of water in the sipper bottle is never exceeded. Prior to sterilization, the chamber is evacuated of air to permit effective distribution of steam. Subsequent to sterilization, the chamber is again evacuated in order to evaporate condensed steam that covers the micro-isolator and its contents. During each of these evacuations, the microprocessor control regulates the rate of vacuum draw down so that the boiling point of water in the sipper bottle is again not exceeded. This method permits micro-isolators to be sterilized with sipper bottles full of water, without losing a significant volume of water.