Abstract: The present disclosure relates to predicting blockages in underground ducts. According to one aspect, there is provided a computer-implemented method of identifying one or more locations along a route for an underground duct which indicate a blockage risk, the route extending between two ends, the method including obtaining a terrain elevation profile for the route; estimating a duct elevation profile for the route based on the terrain elevation profile; and predicting one or more blockage risk locations along the route by determining where water entering the duct from each of the two ends would settle, based on the duct elevation profile.

Abstract: A computer implemented method of allocating a set of communications network resources to resource consumers in a digital communications system can include generating a set of multiple allocation schemes; iteratively applying a genetic algorithm to the set of allocation schemes to generate, at each iteration, a new set of allocation schemes as a generation of allocation schemes based on a preceding generation; and, responsive to a predetermined stopping condition for the iteration, assigning resource consumers to resources in the communications system in accordance with an allocation scheme in a most recent generation determined to satisfy a ranking condition.

Abstract: Nucleases and methods of using these nucleases for alteration of a CFTR gene and generation of cells and animal models.

Abstract: A computer implemented method of navigating a digital representation of a spatial environment by a user in which a portion of the digital representation is rendered by a rendering computer system for visualization by the user. The method including accessing a digital model of the spatial environment including a specification of each of a plurality of virtual resources in the spatial environment, each specification identifying at least a location of a resource in the spatial environment and at least one trigger criterion for determining when a resource is accessed by the user; monitoring the satisfaction of trigger criteria for the plurality of virtual resources; and responsive to the monitoring, determining the portion of the digital representation for rendering by the rendering computer system for visualization by the user.

Abstract: A computer implemented method to specify a software defined network (SDN) for deployment by an SDN controller, the SDN controller being adapted to configure a plurality of physical network components to deliver communication services, the method including generating a model of the physical network components by accessing each of a plurality of data sources; defining the model including an indication of a location, type and interconnections of physical components based on the data sources; associating each indication in the model with a degree of confidence of accuracy of the indication based on the degree of confidence information from the data sources; accessing a set of rules defining relationships between types of physical component; refining the model based on the rules including adjusting a degree of confidence of accuracy of indications in the model based on satisfaction of the rules; and selecting a subset of network components in the refined model for inclusion in the SDN specification.

Abstract: Provided herein are improved methods and compositions for generating CD4-positive effector T cells and regulatory T cells and methods of use thereof.

Abstract: Nucleases and methods of using these nucleases for alteration of a CFTR gene and generation of cells and animal models.

Abstract: Disclosed herein are methods and compositions for inactivating TCR and/or HLA genes, using engineered nucleases comprising at least one DNA binding domain and a cleavage domain or cleavage half-domain in conditions able to preserve cell viability. Polynucleotides encoding nucleases, vectors comprising polynucleotides encoding nucleases and cells comprising polynucleotides encoding nucleases and/or cells comprising nucleases are also provided.

Abstract: The present disclosure is in the field of genome engineering, particularly targeted genetic modification of a CISH gene.

Abstract: Described herein are lipid nanoparticles comprising cationic lipids and other lipids and also comprising engineered nucleases facilitate transfer of nucleic acids to cells.

Abstract: The present disclosure is in the field of genome engineering, particularly targeted genetic modification of a CISH gene.

Abstract: Nucleases and methods of using these nucleases for alteration of a CFTR gene and generation of cells and animal models.

Abstract: A method of maintaining a plurality of infrastructure components as part of a transmission network for a utility service in a defined geographic region having associated environmental characteristics, wherein each infrastructure component is deployed at a location in the region having associated one or more location characteristics and is of a component type having associated one or more infrastructure characteristics. The method including for each infrastructure component, executing a classifier based on one or more of the infrastructure characteristics, the location characteristics, and the environmental characteristics to forecast a measure of susceptibility of the infrastructure component to one or more operational impediments.

Abstract: A method of defining a deployment specification for one or more infrastructure components as part of a transmission network for a utility service in a defined geographic region includes receiving a deployment specification for each of a plurality of deployment options, each received specification including a location and type of one or more infrastructure components for deployment in the region, each type of infrastructure component having associated infrastructure characteristics, and each location having associated location characteristics.

Abstract: Provided herein are genetically engineered mammalian stem and progenitor cells that have increased potential to differentiate into regulatory T cells. Also provided are methods of making and use thereof.

Abstract: Disclosed herein are methods and compositions for inactivating TCR and/or HLA genes, using engineered nucleases comprising at least one DNA binding domain and a cleavage domain or cleavage half-domain in conditions able to preserve cell viability. Polynucleotides encoding nucleases, vectors comprising polynucleotides encoding nucleases and cells comprising polynucleotides encoding nucleases and/or cells comprising nucleases are also provided.

Abstract: A computer implemented method of adapting a software defined network (SDN), the SDN operating with a set of network appliances in communication via network connections and the SDN comprising a control component operable in communication with at least a subset of the network appliances to control communication via the SDN, the method including receiving a representation of the logical arrangement of the SDN including a definition of appliances configured to provide communication for the SDN and network connections therebetween; receiving a measure of a performance metric for the SDN; receiving a forecast demand for the SDN; using a heuristic search to search a library of possible adaptations to the logical arrangement to identify a sequence of adaptations of the SDN that, when implemented, satisfy the forecast demand for the SDN and provide an improved measure of the performance metric of the SDN; applying the identified sequence of adaptations to the SDN by modifying the logical arrangement for use by the con

Abstract: The present disclosure is in the field of genome engineering, particularly targeted integration of a functional SCID-related genes (e.g., IL2RG, RAG1 and/or RAG2 gene) into an IL2RG gene of a cell for provision of proteins lacking or deficient in SCID.

Abstract: Described herein are compositions and methods for modulation of gene expression in the liver including modulation of PCSK9, TTR, SERPINA1, KLKB1 and/or HAO1.

Abstract: Described herein are compositions and methods for modulation of gene expression in the liver including modulation of PCSK9, TTR, SERPINA1, KLKB1 and/or HAO1.