Patents by Inventor Anthony D. Barone
Anthony D. Barone has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20150252351Abstract: The present invention provides methods, compositions, and kits for storing and enhancing the activity of polymerases and particularly thermostable polymerases. The methods comprise mixing a thermostable polymerase with at least one zwitterionic or ylide surfactant that has at least one PEO group. In another aspect the polymerase is mixed with a blocker such as PLURONIC® or TETRONIC® or an amine N-oxide derivative thereof. The thermostable polymerase may be reversibly inactivated by treatment with 2-(Methylsulfonyl)ethyl 4-nitrophenyl carbonate. Compositions and kits for performing the process according to the invention are also provided.Type: ApplicationFiled: May 20, 2015Publication date: September 10, 2015Inventors: Glenn H. McGall, Anthony D. Barone, Christopher J. Kubu
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Patent number: 9085761Abstract: The present invention provides methods, compositions, and kits for storing and enhancing the activity of polymerases and particularly thermostable polymerases. The methods comprise mixing a thermostable polymerase with at least one zwitterionic or ylide surfactant that has at least one PEO group. In another aspect the polymerase is mixed with a blocker such as PLURONIC® or TETRONIC® or an amine N-oxide derivative thereof. The thermostable polymerase may be reversibly inactivated by treatment with 2-(Methylsulfonyl)ethyl 4-nitrophenyl carbonate. Compositions and kits for performing the process according to the invention are also provided.Type: GrantFiled: March 8, 2013Date of Patent: July 21, 2015Assignee: AFFYMETRIX, INC.Inventors: Glenn H. McGall, Anthony D. Barone, Christopher J. Kubu
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Publication number: 20150159198Abstract: The present invention provides methods, compositions, and kits for storing and enhancing the activity of polymerases and particularly thermostable polymerases. The methods comprise mixing a thermostable polymerase with at least one zwitterionic or ylide surfactant that has at least one PEO group. In another aspect the polymerase is mixed with a blocker such as PLURONIC® or TETRONIC® or an amine N-oxide derivative thereof. The thermostable polymerase may be reversibly inactivated by treatment with 2-(Methylsulfonyl)ethyl 4-nitrophenyl carbonate. Compositions and kits for performing the process according to the invention are also provided.Type: ApplicationFiled: February 18, 2015Publication date: June 11, 2015Inventors: Glenn H. McGall, Anthony D. Barone, Christopher J. Kubu
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Patent number: 8497064Abstract: Nucleic acid labeling compounds are disclosed. The compounds are synthesized by condensing a heterocyclic derivative with a cyclic group (e.g. a ribofuranose derivative). The labeling compounds are suitable for enzymatic attachment to a nucleic acid, either terminally or internally, to provide a mechanism of nucleic acid detection.Type: GrantFiled: November 1, 2011Date of Patent: July 30, 2013Assignee: Affymetrix, Inc.Inventors: Glenn H. McGall, Anthony D. Barone
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Publication number: 20120053336Abstract: Nucleic acid labeling compounds are disclosed. The compounds are synthesized by condensing a heterocyclic derivative with a cyclic group (e.g. a ribofuranose derivative). The labeling compounds are suitable for enzymatic attachment to a nucleic acid, either terminally or internally, to provide a mechanism of nucleic acid detection.Type: ApplicationFiled: November 1, 2011Publication date: March 1, 2012Applicant: AFFYMETRIX, INC.Inventors: Glenn McGall, Anthony D. Barone
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Patent number: 8076072Abstract: Nucleic acid labeling compounds including the following are disclosed: These compounds are useful for attaching a detectable label to a nucleic acid.Type: GrantFiled: September 28, 2010Date of Patent: December 13, 2011Assignee: Affymetrix, Inc.Inventors: Glenn Hugh McGall, Anthony D. Barone
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Publication number: 20110143967Abstract: Novel processes are disclosed for forming an array of polymers by functionalizing the surface of particles by methods that include covalently attaching a functionalized silicon compound. Substrates such as microparticles having functionalized silicon compounds attached thereto are produced by introducing at least one carboxyl group directly by silanating a carboxylated silane compound to the surface of a microparticle. In a further aspect of the invention, the silane compound is a dipodal carboxylated silane.Type: ApplicationFiled: December 14, 2010Publication date: June 16, 2011Inventors: Glenn H. McGALL, Anthony D. BARONE, Randall J. TRUE
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Publication number: 20110143966Abstract: Novel processes are disclosed for forming an array of polymers by functionalizing the surface of particles by methods that include covalently attaching a functionalized silicon compound. Substrates such as microparticles having functionalized silicon compounds attached thereto are produced by introducing at least one carboxyl group directly by silanating a carboxylated silane compound to the surface of a microparticle. In a further aspect of the invention, the silane compound is a dipodal carboxylated silane.Type: ApplicationFiled: August 2, 2010Publication date: June 16, 2011Applicant: Affymetrix, INC.Inventors: Glenn H. McGall, Anthony D. Barone
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Publication number: 20110082289Abstract: Nucleic acid labeling compounds are disclosed. The compounds are synthesized by condensing a heterocyclic derivative with a cyclic group (e.g. a ribofuranose derivative). The labeling compounds are suitable for enzymatic attachment to a nucleic acid, either terminally or internally, to provide a mechanism of nucleic acid detection.Type: ApplicationFiled: September 28, 2010Publication date: April 7, 2011Applicant: Affymetric, INC.Inventors: Glenn McGall, Anthony D. Barone
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Publication number: 20110028350Abstract: Novel compounds are provided, which are useful as linking groups in chemical synthesis, preferably in the solid phase synthesis of oligonucleotides and polypeptides. These compounds are generally photolabile and comprise protecting groups which can be removed by photolysis to unmask a reactive group. The protecting group has the general formula Y, wherein Y is a chemical structure as shown in FIG. 5. Also provided is a method of forming, from component molecules, a plurality of compounds on a support, each compound occupying a separate predefined region of the support, using the protected compounds described above.Type: ApplicationFiled: July 28, 2009Publication date: February 3, 2011Applicant: Affymetrix, INC.Inventors: Glenn H. McGall, Anthony D. Barone
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Publication number: 20100324266Abstract: Novel compounds are provided, which are useful as linking groups in chemical synthesis, preferably in the solid phase synthesis of oligonucleotides and polypeptides. These compounds are generally photolabile and comprise protecting groups which can be removed by photolysis to unmask a reactive group. The protecting group has the general formula Y, wherein Y is a chemical structure as shown in FIG. 1. Also provided is a method of forming, from component molecules, a plurality of compounds on a support, each compound occupying a separate predefined region of the support, using the protected compounds described above.Type: ApplicationFiled: June 9, 2010Publication date: December 23, 2010Applicant: Affymetrix, INC.Inventors: Anthony D. Barone, Glenn H. McGall
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Patent number: 7824863Abstract: In one aspect of the invention, a method is provided for end-labeling RNA (total RNA, mRNA, cRNA or fragmented RNA). In one aspect of the present invention, T4 RNA ligase is used to attach a 3?-labeled AMP or CMP donor to an RNA acceptor molecule. In another embodiment, a pyrophosphate molecule 3?-AppN-3?-linker-detectable moiety is used as donor molecule.Type: GrantFiled: October 22, 2008Date of Patent: November 2, 2010Assignee: Affymetrix, Inc.Inventors: Kyle B. Cole, Vivi Truong, Glenn H. McGall, Anthony D. Barone
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Publication number: 20090076295Abstract: Novel compounds are provided, which are useful as linking groups in chemical synthesis, preferably in the solid phase synthesis of oligonucleotides and polypeptides. These compounds are generally photolabile and comprise protecting groups which can be removed by photolysis to unmask a reactive group. The protecting group has the general formula Y, wherein Y is a chemical structure as shown in FIG. 1. Also provided is a method of forming, from component molecules, a plurality of compounds on a support, each compound occupying a separate predefined region of the support, using the protected compounds described above.Type: ApplicationFiled: September 19, 2008Publication date: March 19, 2009Applicant: Affymetrix, INC.Inventors: Anthony D. Barone, Glenn H. McGall
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Patent number: 7504215Abstract: In one aspect of the invention, a method is provided for end-labeling RNA (total RNA, mRNA, cRNA or fragmented RNA). In one aspect of the present invention, T4 RNA ligase is used to attach a 3?-labeled AMP or CMP donor to an RNA acceptor molecule. In another embodiment, a pyrophosphate molecule 3?-AppN-3?-linker-detectable moiety is used as donor molecule.Type: GrantFiled: November 4, 2004Date of Patent: March 17, 2009Assignee: Affymetrix, Inc.Inventors: Kyle B. Cole, Vivi Truong, Glenn H. McGall, Anthony D. Barone
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Publication number: 20090054258Abstract: In one aspect of the invention, a method is provided for end-labeling RNA (total RNA, mRNA, cRNA or fragmented RNA). In one aspect of the present invention, T4 RNA ligase is used to attach a 3?-labeled AMP or CMP donor to an RNA acceptor molecule. In another embodiment, a pyrophosphate molecule 3?-AppN-3?-linker-detectable moiety is used as donor molecule.Type: ApplicationFiled: October 22, 2008Publication date: February 26, 2009Applicant: Affymetrix, IncInventors: Kyle B. Cole, Vivi Truong, Glenn H. McGall, Anthony D. Barone
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Patent number: 7491818Abstract: Nucleic acid labeling compounds containing heterocyclic derivatives are disclosed. The heterocyclic derivative containing compounds are synthesized by condensing a heterocyclic derivative with a cyclic group (e.g. a ribofuranose derivative). The labeling compounds are suitable for enzymatic attachment to a nucleic acid, either terminally or internally, to provide a mechanism of nucleic acid detection.Type: GrantFiled: January 18, 2005Date of Patent: February 17, 2009Assignee: Affymetrix, Inc.Inventors: Glenn McGall, Anthony D. Barone
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Patent number: 7468243Abstract: Nucleic acid labeling compounds are disclosed having the formula: These compounds are useful for attaching a detectable label to a nucleic acid.Type: GrantFiled: March 12, 2002Date of Patent: December 23, 2008Assignee: Affymetrix, Inc.Inventors: Glenn McGall, Anthony D. Barone
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Publication number: 20080287667Abstract: Nucleic acid labeling compounds are disclosed. The compounds are synthesized by condensing a heterocyclic derivative with a cyclic group (e.g. a ribofaranose derivative). The labeling compounds are suitable for enzymatic attachment to a nucleic acid, either terminally or internally, to provide a mechanism of nucleic acid detection.Type: ApplicationFiled: June 27, 2008Publication date: November 20, 2008Applicant: Affymetrix, INC.Inventors: Glenn McGall, Anthony D. Barone
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Patent number: 7423143Abstract: Nucleic acid labeling compounds containing heterocyclic derivatives are disclosed. The heterocyclic derivative containing compounds are synthesized by condensing a heterocyclic derivative with a cyclic group (e.g. a ribofuranose derivative). The labeling compounds are suitable for enzymatic attachment to a nucleic acid, either terminally or internally, to provide a mechanism of nucleic acid detection.Type: GrantFiled: May 10, 2005Date of Patent: September 9, 2008Assignee: Affymetrix. Inc.Inventors: Glenn H. McGall, Anthony D. Barone
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Patent number: 7291463Abstract: A method for converting a pseudoisocytidine base having the formula: to a pseudoisocytidine molecule having the formula: is disclosed. Such compounds are useful as nucleic acid labeling compounds.Type: GrantFiled: December 23, 2003Date of Patent: November 6, 2007Assignee: Affymetrix, Inc.Inventors: Glenn H. McGall, Anthony D. Barone, Handong Li