Abstract: THE present application relates to a method of detecting one or more analytes in a sample, the method comprising (a) providing a carrier nucleic acid molecule with at least one single-stranded region; (b) providing one or more aptamers specific for the analyte, wherein the aptamers additionally comprise a single-stranded portion complementary to at least one single-stranded region on the carrier nucleic acid; (c) contacting the carrier nucleic acid and one or more aptamers with the sample, forming a carrier nucleic acid/N aptamer/analyte complex, and; (d) detecting the presence of the carrier nucleic acid/aptamer/analyte complex.

Abstract: The present invention is a device for detecting the presence of an analyte in a sample. The device comprises (i) at least one electrode, (ii) an oxidase enzyme, and (iii) first and second redox mediators.

Abstract: THE present application relates to a method of detecting one or more analytes in a sample, the method comprising (a) providing a carrier nucleic acid molecule with at least one single-stranded region; (b) providing one or more aptamers specific for the analyte, wherein the aptamers additionally comprise a single-stranded portion complementary to at least one single-stranded region on the carrier nucleic acid; (c) contacting the carrier nucleic acid and one or more aptamers with the sample, forming a carrier nucleic acid/N aptamer/analyte complex, and; (d) detecting the presence of the carrier nucleic acid/aptamer/analyte complex.

Abstract: The present invention provides a device for detecting the presence of an analyte in a sample, said device comprising (i) at least one electrode, (ii) a oxidase enzyme, and (iii) first and second redox mediators.

Abstract: The present invention provides an arsenite oxidase enzyme modified to prevent translocation by modification of a translocation signal sequence. A microorganism modified to express the heterologous arsenite oxidase enzyme is also provided by the invention, together with a device for detecting the presence of arsenite in a sample.

Abstract: The invention provides an apparatus and methods for the electrochemical detection and/or quantitation of an analyte in a sample, wherein the device comprises a substrate and a detector, wherein the substrate comprises a labeled binding agent, wherein the label is an enteric material particle that encapsulates a ferrocene methanol redox species.

Abstract: The present invention provides an arsenite oxidase enzyme modified to prevent translocation by modification of a translocation signal sequence. A microorganism modified to express the heterologous arsenite oxidase enzyme is also provided by the invention, together with a device for detecting the presence of arsenite in a sample.

Abstract: The invention provides an apparatus and methods for the electrochemical detection and/or quantitation of an analyte in a sample, wherein the device comprises a substrate and a detector, wherein the substrate comprises a labeled binding agent, wherein the label is an enteric material particle that encapsulates a ferrocene methanol redox species.

Abstract: The present invention provides an arsenite oxidase enzyme modified to prevent translocation by modification of a translocation signal sequence. A microorganism modified to express the heterologous arsenite oxidase enzyme is also provided by the invention, together with a device for detecting the presence of arsenite in a sample.

Abstract: Provided is a peptide and a hydrogel prepared therefrom. The peptide comprises the sequence Gly-(A)-(Gly-X-Y)m-M-(Gly-X-Y)n-Gly-(B)-Gly, wherein A and B are telopeptides, X and Y are any amino acid, m and n are integers of at least 3 and M is a receptor binding sequence. Artificial collagen derived from the peptide of the invention provides an alternative to the use of animal- or human-derived collagen in industrial, biological and biomedical applications.

Abstract: The invention provides methods of purifying one or more biological analytes from a sample. The method comprises incubating the sample with nanoparticles and centrifuging a biphasic system. The biological analytes can be one or more of an enzyme, a protein, a nucleic acid, an organelle, a cell, a bacteria, a virus, or any other biological material which requires purification.

Abstract: The invention provides apparatus for the quantitative analysis of an analyte in a sample, comprising (i) a solid phase; and (ii) a detector, wherein the surface of the solid phase comprises (a)a first position for sample application, and (b)a second position, distant from the first position, wherein a first molecule that binds to the analyte and is capable of releasing a detectable species is either deposited at the first position or is added to the sample prior to application to the LF membrane, and wherein a second molecule that binds to the analyte is immobilised at the second position, and wherein an enzyme is immobilised, co-located with the immobilised molecule at the second position, and wherein the detector is located in close proximity to the immobilised molecule at the second position.

Abstract: The present invention provides an arsenite oxidase enzyme modified to prevent translocation by modification of a translocation signal sequence. A microorganism modified to express the heterologous arsenite oxidase enzyme is also provided by the invention, together with a device for detecting the presence of arsenite in a sample.

Abstract: An apparatus and method for analysing temperature-dependent molecular configurations such as folding comprises a multi-channel flow-through chip (12) along which molecules to be analysed pass. A temperature gradient is maintained along the length of the chip. As molecules pass along the channels they fold or unfold, in response to the changing temperature. These changing molecular configurations are investigated by simultaneously measuring the extent to which the molecules absorb UV light, and the extent to which they fluoresce. The absorption and fluorescence information is supplied to a computer system (26) for real-time analysis.

Abstract: A process is described for detecting modulation of NAD(P)H and/or a NAD(P)H dependent oxidoreductase (“NDO”) in or by a biological system which comprises NAD(P)H and an NDO. The process comprises generating a detectable marker of NAD(P)H modulation, and detecting said detectable marker.

Abstract: A process is described for detecting modulation of NAD(P)H and/or a NAD(P)H dependent oxidoreductase (“NDO”) in or by a biological system which comprises NAD(P)H and an NDO. The process comprises generating a detectable marker of NAD(P)H modulation, and detecting said detectable marker.