Patents by Inventor Anthony J. Sinskey
Anthony J. Sinskey has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 8314071Abstract: Certain aspects of the invention relate to antibiotics, as well as pharmaceutically acceptable salts, pro-drugs and/or analogs thereof. Another aspect of the invention relates to methods of use of said antibiotics.Type: GrantFiled: December 22, 2008Date of Patent: November 20, 2012Assignee: Massachusetts Institute of TechnologyInventors: Anthony J. Sinskey, Philip A. Lessard, Kazuhiro Kurosawa
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Publication number: 20120238773Abstract: A composition comprising caffeoylshikimic acids, protocatechuic acid, hydroxytyrosol, hydroxybenzoic acid, said caffeoylshikimic acids and their derivatives extracted from any part of oil palm including but not confined to the vegetation liquor of palm oil milling and palm oil mill effluent, and a method for use in the preparation of a composition containing caffeoylshikimic acids, protocatechuic acid, hydroxytyrosol, hydroxybenzoic acid, said caffeoylshikimic acids and their derivatives.Type: ApplicationFiled: May 26, 2010Publication date: September 20, 2012Inventors: T.G. Sambandan, ChoKyun Rha, Anthony J. Sinskey, Ravigadevi Sambanthamurthi, Yew Ai Tan, Kalyana Sundram P. Manickam, Mohd Basri Wahid
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Publication number: 20100249051Abstract: Certain aspects of the invention relates to antibiotics, as well as pharmaceutically acceptable salts, pro-drugs and/or analogs thereof. Another aspect of the inventions relates to methods of use of said antibiotics.Type: ApplicationFiled: December 22, 2008Publication date: September 30, 2010Applicant: Massachusetts Institute of TechnologyInventors: Anthony J. Sinskey, Philip A. Lessard, Kazuhiko Kurosawa
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Patent number: 7300777Abstract: The present invention relates to a mutated pyruvate carboxylase gene from Corynebacterium. The mutant pyruvate carboxylase gene encodes a pyruvate carboxylase enzyme which is resistant to feedback inhibition from aspartic acid. The present invention also relates to a method of replacing the wild-type pyruvate carboxylase gene in Corynebacterium with this feedback-resistant pyruvate carboxylase gene. The present invention further relates to methods of the production of amino acids, preferably lysine, comprising the use of this mutant pyruvate carboxylase enzyme in microorganisms.Type: GrantFiled: May 25, 2005Date of Patent: November 27, 2007Assignees: Archer-Daniels-Midland Company, Massachusetts Institute of TechnologyInventors: Paul D. Hanke, Anthony J. Sinskey, Laura B. Willis, Stephane Guillouet
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Patent number: 7109007Abstract: A method for controlling and modifying biopolymer synthesis by manipulation of the genetics and enzymology of synthesis of polyhydroxybutyrate (PHB) and polyhydroxyalkanoate (PHA) polyesters at the molecular level in procaryotic and eukaryotic cells, especially plants. Examples demonstrate the isolation, characterization, and expression of the genes involved in the production of PHB and PHA polymers. Genes encoding the enzymes in the PHB and PHA synthetic pathway (beta-ketothiolase, acetoacetyl-CoA reductase and PHB polymerase or PHA polymerase) from Zoogloea ramigera strain I-16-M, Alcaligenes eutrophus, Nocardia salmonicolur, and Psuedomonas olevarans were identified or isolated and expressed in a non-PHB producing organism, E. coli. Specific modifications to the polymers include variation in the chain length of the polymers and incorporation of different monomers into the polymers to produce co-polymers with different physical properties.Type: GrantFiled: March 22, 2005Date of Patent: September 19, 2006Assignee: Massachusetts Institute of TechnologyInventors: Oliver P. Peoples, Anthony J. Sinskey
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Patent number: 6987017Abstract: The present invention relates, in general, to the over-production of L-isoleucine by nonhuman organisms. More specifically, the present invention relates to methods for producing L-isoleucine comprising: (a) growing a transformed nonhuman organism under conditions that provide for synthesis of L-isoleucine, wherein the nonhuman organism comprises one or more copies of a transgene comprising at least one nucleotide sequence encoding catabolic threonine dehydratase; wherein the L-isoleucine is synthesized by the transformed nonhuman organism, the synthesis being greater than that of the corresponding non-transformed nonhuman organism; and (b) recovering the L-isoleucine from the culture medium in which the transformed nonhuman organism was cultured.Type: GrantFiled: August 20, 2002Date of Patent: January 17, 2006Assignee: Massachusetts Institute of TechnologyInventors: Stephane Guillouet, Anthony J. Sinskey, Avital A. Rodal, Philip A. Lessard
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Patent number: 6965021Abstract: The present invention relates to a mutated pyruvate carboxylase gene from Corynebacterium. The mutant pyruvate carboxylase gene encodes a pyruvate carboxylase enzyme which is resistant to feedback inhibition from aspartic acid. The present invention also relates to a method of replacing the wild-type pyruvate carboxylase gene in Corynebacterium with this feedback-resistant pyruvate carboxylase gene. The present invention further relates to methods of the production of amino acids, preferably lysine, comprising the use of this mutant pyruvate carboxylase enzyme in microorganisms.Type: GrantFiled: October 12, 2001Date of Patent: November 15, 2005Assignees: Archer-Daniels-Midland Company, Massachusetts Institute of TechnologyInventors: Paul D. Hanke, Anthony J. Sinskey, Laura B. Willis
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Patent number: 6884606Abstract: The present invention concerns an anaplerotic enzyme from Corynebacterium glutamicum which replenishes oxaloacetate consumed during lysine and glutamic acid production in industrial fermentations. In particular, isolated nucleic acid molecules are provided encoding the pyruvate carboxylase protein. Pyruvate carboxylase polypeptides are also provided.Type: GrantFiled: January 15, 2002Date of Patent: April 26, 2005Assignee: Massachusetts Institute of TechnologyInventors: Anthony J. Sinskey, Philip A. Lessard, Laura B. Willis
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Patent number: 6881560Abstract: A method for controlling and modifying biopolymer synthesis by manipulation of the genetics and enzymology of synthesis of polyhydroxybutyrate (PHB) and polyhydroxyalkanoate (PHA) polyesters at the molecular level in procaryotic and eukaryotic cells, especially plants. Examples demonstrate the isolation, characterization, and expression of the genes involved in the production of PHB and PHA polymers. Genes encoding the enzymes in the PHB and PHA synthetic pathway (beta-ketothiolase, acetoacetyl-CoA reductase and PHB polymerise or PHA polymerase) from Zooloea ramigera strain I-16-M, Alcaligenes eutrophus, Nocardia salmonicolur, and Psuedomnas olevarans were identified or isolated and expressed in a non-PHB producing organism, E. coli. Specific modifications to the polymers include variation in the chain length of the polymers and incorporation of different monomers into the polymers to produce co-polymers with different physical properties.Type: GrantFiled: January 13, 2003Date of Patent: April 19, 2005Assignee: Massachusetts Institute of TechnologyInventors: Oliver P. Peoples, Anthony J. Sinskey
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Publication number: 20040077075Abstract: The present invention provides a variety of microscale bioreactors (microfermentors) and microscale bioreactor arrays for use in culturing cells. The microfermentors include a vessel for culturing cells and means for providing oxygen to the interior of the vessel at a concentration sufficient to support cell growth, e.g., growth of bacterial cells. Depending on the embodiment, the microfermentor vessel may have various interior volumes less than approximately 1 ml. The microfermentors may include an aeration membrane and optionally a variety of sensing devices. The invention further provides a chamber to contain the microfermentors and microfermentor arrays and to provide environmental control. Certain of the microfermentors include a second chamber that may be used, e.g., to provide oxygen, nutrients, pH control, etc., to the culture vessel and/or to remove metabolites, etc. Various methods of using the microfermentors, e.g., to select optimum cell strains or bioprocess parameters are provided.Type: ApplicationFiled: May 1, 2003Publication date: April 22, 2004Applicant: Massachusetts Institute of TechnologyInventors: Klavs F. Jensen, Paul Laibinis, Rajeev J. Ram, Anthony J. Sinskey, Nicolas Szita, Andrea Zanzotto, Martin A. Schmidt
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Patent number: 6649379Abstract: Mutagenesis of the gene encoding homoserine dehydrogenase (hom) for production of the amino acid threonine is described. The mutation causes an alteration in the carboxy terminus of the enzyme that interferes with end-product inhibition by threonine. The lack of end-product inhibition causes an overproduction of threonine.Type: GrantFiled: November 1, 1991Date of Patent: November 18, 2003Assignee: Massachusetts Institute of TechnologyInventors: John A. C. Archer, Maximillian T. Follettie, Anthony J. Sinskey
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Patent number: 6528706Abstract: A method for controlling and modifying biopolymer synthesis by manipulation of the genetics and enzymology of synthesis of polyhydroxybutyrate (PHB) and polyhydroxyalkanoate (PHA) polyesters at the molecular level in procaryotic and eukaryotic cells, especially plants. Examples demonstrate the isolation, characterization, and expression of the genes involved in the production of PHB and PHA polymers. Genes encoding the enzymes in the PHB and PHA synthetic pathway (beta-ketothiolase, acetoacetyl-CoA reductase and PHB polymerase or PHA polymerase) from Zoogloea ramigera strain I-16-M, Alcaligenes eutrophus, Nocardia salmonicolur, and Psuedomonas olevarans were identified or isolated and expressed in a non-PHB producing organism, E. coli. Specific modifications to the polymers include variation in the chain length of the polymers and incorporation of different monomers into the polymers to produce co-polymers with different physical properties.Type: GrantFiled: August 21, 2001Date of Patent: March 4, 2003Assignee: Massachusetts Institute of TechnologyInventors: Oliver P. Peoples, Anthony J. Sinskey
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Publication number: 20030027305Abstract: The present invention concerns an anaplerotic enzyme from Corynebacterium glutamicum which replenishes oxaloacetate consumed during lysine and glutamic acid production in industrial fermentations. In particular, isolated nucleic acid molecules are provided encoding the pyruvate carboxylase protein. Pyruvate carboxylase polypeptides are also provided.Type: ApplicationFiled: January 15, 2002Publication date: February 6, 2003Applicant: Archer-Daniels-Midland CompanyInventors: Anthony J. Sinskey, Philip A. Lessard, Laura B. Willis
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Publication number: 20030008359Abstract: The present invention relates, in general, to the over-production of L-isoleucine by nonhuman organisms.Type: ApplicationFiled: August 20, 2002Publication date: January 9, 2003Applicant: Massachussetts Institute of TechnologyInventors: Stephane Guillouet, Anthony J. Sinskey, Avital A. Rodal, Philip A. Lessard
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Patent number: 6451564Abstract: The present invention relates, in general, to the over-production of L-isoleucine by nonhuman organisms. More specifically, the present invention relates to methods for producing L-isoleucine comprising: (a) growing a transformed nonhuman organism under conditions that provide for synthesis of L-isoleucine, wherein the nonhuman organism comprises one or more copies of a transgene comprising at least one nucleotide sequence encoding catabolic threonine dehydratase; wherein the L-isoleucine is synthesized by the transformed nonhuman organism, the synthesis being greater than that of the corresponding non-transformed nonhuman organism; and (b) recovering the L-isoleucine from the culture medium in which the transformed nonhuman organism was cultured.Type: GrantFiled: June 30, 2000Date of Patent: September 17, 2002Assignee: Massachusetts Institute of TechnologyInventors: Stephane Guillouet, Avital A. Rodal, Philip A. Lessard, Anthony J. Sinskey
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Patent number: 6403351Abstract: The present invention concerns an anaplerotic enzyme from Corynebacterium glutamicum which replenishes oxaloacetate consumed during lysine and glutamic acid production in industrial fermentations. In particular, isolated nucleic acid molecules are provided encoding the pyruvate carboxylase protein. Pyruvate carboxylase polypeptides are also provided.Type: GrantFiled: October 3, 2000Date of Patent: June 11, 2002Assignee: Archer Daniels Midland CompanyInventors: Anthony J. Sinskey, Philip A. Lessard, Laura B. Willis
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Patent number: 6171833Abstract: The present invention concerns an anaplerotic enzyme from Corynebacterium glutamicum which replenishes oxaloacetate consumed during lysine and glutamic acid production in industrial fermentations. In particular, isolated nucleic acid molecules are provided encoding the pyruvate carboxylase protein. Pyruvate carboxylase polypeptides are also provided.Type: GrantFiled: December 23, 1998Date of Patent: January 9, 2001Assignee: Massachusetts Institute of TechnologyInventors: Anthony J. Sinskey, Philip A. Lessard, Laura B. Willis
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Patent number: 5811272Abstract: A method has been developed for control of molecular weight and molecular weight dispersity during production of polyhydroxyalkanoates in genetically engineered organism by control of the level and time of expression of one or more PHA synthases in the organisms. The method was demonstrated by constructing a synthetic operon for PHA production in E. coli in which the level of PHA synthase activity could be tightly controlled by placement of the synthase behind an inducible promoter. Modulation of the total level of PHA synthase activity in the host cell by varying the concentration of the inducer, isopropyl .beta.-D-thiogalactoside (IPTG), was found to effect the molecular weight of the polymer produced in the cell. Specifically, high concentrations of synthase activity were found to yield polymers of low molecular weight while low concentrations of synthase activity yielded polymers of higher molecular weight.Type: GrantFiled: July 26, 1996Date of Patent: September 22, 1998Assignee: Massachusetts Institute of TechnologyInventors: Kristi D. Snell, Scott A. Hogan, Sang Jun Sim, Anthony J. Sinskey, Chokyun Rha
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Patent number: 5798235Abstract: The present invention is a method for controlling biopolymer synthesis by determining the genetics and enzymology of polyhydroxybutyrate (PHB) biosynthesis at the molecular level. The purified enzymes and genes provide the means for developing new PHB-like biopolymers having polyester backbones. Specific aims are to 1) control the chain length of the polymers produced in fermentation processes through genetic manipulation, 2) incorporate different monomers into the polymers to produce copolymers with different physical properties, and 3) examine the physical/rheological properties of these new biopolymers in order to develop further design criteria at the molecular level.Type: GrantFiled: June 7, 1995Date of Patent: August 25, 1998Assignee: Massachusetts Institute of TechnologyInventors: Oliver P. Peoples, Anthony J. Sinskey
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Patent number: 5663063Abstract: A method for controlling and modifying biopolymer synthesis by manipulation of the genetics and enzymology of synthesis of polyhydroxybutyrate (PHB) and polyhydroxyalkanoate (PHA) polyesters at the molecular level in procaryotic and eukaryotic cells, especially plants. Examples demonstrate the isolation, characterization, and expression of the genes involved in the production of PHB and PHA polymers. Genes encoding the enzymes in the PHB and PHA synthetic pathway (beta-ketothiolase, acetoacetyl-CoA reductase and PHB polymerase or PHA polymerase) from Zoogloea ramigera strain I-16-M, Alcaligenes eutrophus, Nocardia salmonicolur, and Psuedomonas olevarans were identified or isolated and expressed in a non-PHB producing organism, E. coli. Specific modifications to the polymers include variation in the chain length of the polymers and incorporation of different monomers into the polymers to produce co-polymers with different physical properties.Type: GrantFiled: May 10, 1995Date of Patent: September 2, 1997Assignee: Massachusetts Institute of TechnologyInventors: Oliver P. Peoples, Anthony J. Sinskey