Abstract: An agile nucleic acid sensor includes: a DNA switch; an analysis substrate in electrostatic communication with the DNA switch and that produces a biomarker electrical signal; a transduction member that receives the biomarker electrical signal and produces a transduction signal; a sensor counter electrode in electrical communication with and capacitively coupled to the analysis substrate and that receives a counter electrode voltage; a sensor reference electrode in electrical communication and capacitively coupled to the analysis substrate and that produces a feedback signal based on electrical interactions with a composition that is in fluid contact with the feedback signal and the analysis substrate; and a voltage follower in electrical communication with the sensor counter electrode and the sensor reference electrode and that receives the feedback signal from the sensor reference electrode and produces the counter electrode voltage for the sensor counter electrode based on the feedback signal.

Abstract: Embodiments of the present invention relate to a system and method for determining quantity of target nucleic acid sequence in a sample. During a PCR-based amplification reaction, fluorescence intensity signals are acquired that form an amplification profile from which an exponential amplification region is desirably identified. In determining the exponential region, embodiments of the present invention determine a fluorescence threshold by background subtraction, test the feasibility of matching a signal to a reference curve and, in the event the feasibility test is successful, determine the matching parameters that quantify the initial amplicon number, and signal detection that reduces systematic errors in the measurements and increase the sensitivity of the measurement by decreasing the apparent noise-floor.

Abstract: Embodiments of the present invention described herein provide a device that can measure a single particle in flow several times along a microchannel with integrated waveguides that carry optical signals (e.g. excitation, emission, transmission, and scattered light) to and from measurement regions. Embodiments of the present invention used to perform multiple measurements of particles, such as microspheres or cells, traveling in a sample fluid through a microfluidic channel achieve lower uncertainties, discriminate complex samples, and account for sources of uncertainty that might be related to the shape, deformability, stability, or activity of objects in a liquid sample.

Abstract: An optical flow meter includes a substrate; a microchannel with a fluid receiver; a fluid transmitter; a fluid member with an optical interaction region; a photo interaction region; an analytical light path, such that analytical light interacts with an analyte in a photo interaction region subsequent to an interaction of a pre-analyte with activation light in an optical interaction region to produce analyte; and a detection light path disposed in the substrate, arranged at an oblique angle or right angle to the fluid member proximate to the photo interaction region, and that: receives the photoanalyte light from the photo interaction region; and communicates the photoanalyte light from the microchannel to a photodetector, the optical flow meter determines a flow rate of the analyte.

Abstract: A multiplexed amplitude modulation photometer includes a microchannel; a first input light path that: receives a first modulated light at a first modulation frequency; and communicates the first modulated light to a first optical region that receives the first analyte that produces a first output light including the first modulation frequency communicated to a first detection light path; the first optical region; the first detection light path that receives the first output light; a second input light path that: receives a second modulated light with a second modulation frequency; and communicates second modulated light to a second optical region that receives the second analyte that produces a second output light with the second modulation frequency communicated to a second detection light path; the second optical region; and the second detection light path that receives the second output light from the second optical region.

Abstract: An optical flow meter includes a substrate; a microchannel with a fluid receiver; a fluid transmitter; a fluid member with an optical interaction region; a photo interaction region; an analytical light path, such that analytical light interacts with an analyte in a photo interaction region subsequent to an interaction of a pre-analyte with activation light in an optical interaction region to produce analyte; and a detection light path disposed in the substrate, arranged at an oblique angle or right angle to the fluid member proximate to the photo interaction region, and that: receives the photoanalyte light from the photo interaction region; and communicates the photoanalyte light from the microchannel to a photodetector, the optical flow meter determines a flow rate of the analyte.