Abstract: This invention relates to the amplification of multiple nucleic acid target sequences. Forward and reverse compound primers comprising a common amplification sequence and a target-specific primer sequence are immobilized at a site on a solid support. Target regions of single-stranded template DNA are primed and copied by the forward primer to produce a first extension product. The template DNA is removed and the first extension product is primed and copied by the reverse primer to produce a second extension product. The second extension product has common amplification sequences at each end and is bulk-amplified in solution by regular PCR employing primers that target the common amplification sequences. These methods allow highly multiplexed amplifications to be performed.

Abstract: This invention relates to the amplification of multiple nucleic acid target sequences. Forward and reverse compound primers comprising a common amplification sequence and a target-specific primer sequence are immobilised at a site on a solid support. Target regions of single-stranded template DNA are primed and copied by the forward primer to produce a first extension product. The template DNA is removed and the first extension product is primed and copied by the reverse primer to produce a second extension product. The second extension product has common amplification sequences at each end and is bulk-amplified in solution by regular PCR employing primers that target the common amplification sequences. These methods allow highly multiplexed amplifications to be performed.