Abstract: In accordance with some embodiments of the disclosed subject matter, provided herein are mechanisms (which can, for example, include systems, methods, and media) for automatically interpreting and classifying images of Gram-stained biological samples.

Abstract: Disclosed are mutated genes for green fluorescence proteins and enhanced inserted YFPs expressed therefrom. The mutant proteins not only maintain their fluorescence even at 37° C., but also exhibit about 20 times stronger fluorescence intensities in comparison to the conventional fluorescence proteins. Accordingly, the mutant fluorescence proteins of the present invention can be used as biosensors for detecting and analyzing the bioactivities of desired materials.