Abstract: A method for bioprocessing includes the steps of providing a bioprocessing system having a first bioreactor vessel and a second bioreactor vessel, activating a population of cells in the first bioreactor vessel, genetically modifying the population of cells to produce a population of genetically modified cells, and expanding the population of genetically modified cells within the first bioreactor vessel and the second bioreactor vessel.

Abstract: A kit for magnetic cell isolation includes first stopcock manifold having at least four stopcocks, a separation chamber configured for use with a centrifugal processing chamber of the cell processing device, the separation chamber in fluid communication with the first stopcock manifold, a mixing bag configured for use with a heating/cooling mixing chamber of a cell processing device, the mixing bag in fluid communication with the first stopcock manifold, a second stopcock manifold having at least four stopcocks, the second stopcock manifold in fluid communication with the first stopcock manifold, a magnetic cell isolation holder in fluid communication with the second stopcock manifold, the magnetic cell isolation holder configured for use with a magnetic field generator of a magnetic cell isolation device, and a plurality of cell processing bags in fluid communication with the first and/or second stopcock manifolds.

Abstract: Separating and singulating embryos employs a spray module configured to spray a plurality of embryos which are loaded on a porous substrate so as to separate and singulate the plurality of embryos, and a drying module configured to dry the plurality of separated and singulated embryos retained on the porous substrate while the porous substrate is moved across the drying module. A robotic arm operates to transfer the porous substrate from module to module, and a control device controls the operation of the system of separating and singulating embryos.

Abstract: A method of improving germination of plant embryos is provided. The method entails treating a culture of plant tissue with a liquid multiplication medium comprising a plant non-metabolizable sugar. Also provided is a multiplication medium for liquid cultures of plant cells comprising isomaltulose.

Abstract: A method of sinuglating embryos is provided. The method includes providing a plurality of embryos (40) within a system (20) and sensing (34) at least one of the plurality of embryos in a fluid. The method also includes dispensing (26) at least one of the plurality of embryos on a surface (28).

Abstract: Separating and singulating embryos employs a spray module configured to spray a plurality of embryos which are loaded on a porous substrate so as to separate and singulate the plurality of embryos, and a drying module configured to dry the plurality of separated and singulated embryos retained on the porous substrate while the porous substrate is moved across the drying module. A robotic arm operates to transfer the porous substrate from module to module, and a control device controls the operation of the system of separating and singulating embryos.

Abstract: The present invention is directed to a spray apparatus and methods for the separation of plant embryos.

Abstract: A system for harvesting totipotent plant tissue culture can include a sterile enclosure, a plurality of bioreactors in the sterile enclosure, at least one agitator in the sterile enclosure, and a culture harvest system. The at least one agitator can be configured to agitate culture within the plurality of bioreactors. The culture harvest system can include tubing configured to connect to a port of one of the plurality of bioreactors in the sterile enclosure, a culture harvest container, and a pump configured to pump culture from the one of the plurality of bioreactors into the culture harvest container via the tubing.

Abstract: A totipotent plant tissue multiplication system can include a sterile enclosure and a bioreactor in the sterile enclosure. The bioreactor can include a number of ports. A sensor can be inserted into a first port. A matter insertion system can input matter into the bioreactor via tubing configured to be connected to a second port. The sensor can be removed from the first port during operation of the matter insertion system. The tubing can be disconnected from the second port during operating of the sensor and the culture transfer system.

Abstract: A totipotent plant tissue multiplication system can include a plurality of bioreactors, an agitator to agitate culture in the bioreactors, and a gas source and control system. The gas source and control system can include a dissolved gas sensor in each of the bioreactors, gas sources, a manifold connected to each of the gas sources, and valves connected to the manifold. Each of the valves can be connected to one of the bioreactors. A controller can open the valves and control amounts of gas released from the gas sources into the manifold based on signals received from the dissolved gas sensors in each of the bioreactors.

Abstract: The present invention is directed to an automated system and methods for separating and singulating plant embryos.

Abstract: The present invention provides methods of multiplying plant embryogenic tissue in a bioreactor.

Abstract: A method of sinuglating embryos is provided. The method includes providing a plurality of embryos (40) within a system (20) and sensing (34) at least one of the plurality of embryos in a fluid. The method also includes dispensing (26) at least one of the plurality of embryos on a surface (28).

Abstract: A method of singulating embryos is provided. The method includes providing a plurality of embryos (40) within a system (20) and sensing (34) at least one of the plurality of embryos in a fluid. The method also includes dispensing (26) at least one of the plurality of embryos on a surface (28).

Abstract: The present invention provides methods of multiplying plant embryogenic tissue in a bioreactor.

Abstract: A method of singulating embryos is provided. The method includes providing a plurality of embryos (40) within a system (20) and sensing (34) at least one of the plurality of embryos in a fluid. The method also includes dispensing (26) at least one of the plurality of embryos on a surface (28).