Abstract: A vortexing chamber, including: a chamber housing having a hollow channel, a first end and a second end; and one or more structural impediment objects having a substantially spherical, cubic, rectangular, cylindrical, polyhedron, tetrahedron, or irregular shape; where the objects are housed within the hollow channel, configured to mix a liquid and gas (for example, oxygen or nitrogen) when a liquid and gas pass through the vortexing chamber. The structural impediment objects can provide turbulence and dispersion when a liquid and gas are passed through the vortexing chamber at a high velocity, resulting in micro-bubbles or nano-bubbles suspended in a liquid and gas mixture.

Abstract: The present invention relates generally to improved methods for tissue engineering including tissue transplantation, augmentation and regeneration. More particularly, the present invention provides a method for the generation of donor vascularized tissue suitable for use in tissue transplantation, augmentation and/or repair. The present invention further enables the use of a support matrix in the generation of an anatomical construct comprising the donor vascular tissue. The support matrix may be devised such that it has dimensions of a size and shape adapted to simulate those of tissue to be transplanted, augmented and/or repaired. In addition to its use in tissue repair, the methods and support matrix of the present invention may also find application as a means for delivering a desirable gene product to a subject. The method and support matrix of the present invention is conveniently be made available in the form of a kit, for use generally in the field of tissue engineering.

Abstract: The present invention relates generally to improved methods for tissue engineering including tissue transplantation, augmentation and regeneration. More particularly, the present invention provides a method for the generation of donor vascularized tissue suitable for use in tissue transplantation, augmentation and/or repair. The present invention further enables the use of a support matrix in the generation of an anatomical construct comprising the donor vascular tissue. The support matrix may be devised such that it has dimensions of a size and shape adapted to simulate those of tissue to be transplanted, augmented and/or repaired. In addition to its use in tissue repair, the methods and support matrix of the present invention may also find application as a means for delivering a desirable gene product to a subject. The method and support matrix of the present invention is conveniently be made available in the form of a kit, for use generally in the field of tissue engineering.