Patents by Inventor Anthony Scozzari

Anthony Scozzari has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Oligonucleotide synthesis with alternative solvents
    Patent number: 7276599
    Abstract: The invention provides for methods of manufacturing an oligonucleotide comprising a pentavalent phosphate triester. In particular, the method comprises providing a 5? blocked-nucleoside, deblocking the 5? blocked-nucleoside to form a 5? OH-nucleoside, coupling the 5? OH-nucleoside with a phosphoramidite to form and oligonucleotide comprising a trivalent phosphite triester; and oxidizing the oligonucleotide comprising a trivalent phosphite triester to the oligonucleotide comprising a pentavalent phosphate triester. In some embodiments, the wash between any of the steps above is with at least one solvent wash comprising a toluene.
    Type: Grant
    Filed: June 2, 2004
    Date of Patent: October 2, 2007
    Assignee: Isis Pharmaceuticals, Inc.
    Inventors: Max N. Moore, Mark Andrade, Recaldo Carty, Anthony Scozzari, Achim Krotz
  • Oligonucleotide synthesis with alternative solvents
    Publication number: 20050026192
    Abstract: The invention provides for methods of manufacturing an oligonucleotide comprising a pentavalent phosphate triester. In particular, the method comprises providing a 5? blocked-nucleoside, deblocking the 5? blocked-nucleoside to form a 5? OH-nucleoside, coupling the 5? OH-nucleoside with a phosphoramidite to form and oligonucleotide comprising a trivalent phosphite triester; and oxidizing the oligonucleotide comprising a trivalent phosphite triester to the oligonucleotide comprising a pentavalent phosphate triester. In some embodiments, the wash between any of the steps above is with at least one solvent wash comprising a toluene.
    Type: Application
    Filed: June 2, 2004
    Publication date: February 3, 2005
    Applicant: ISIS Pharmaceuticals, Inc.
    Inventors: Max Moore, Mark Andrade, Recaldo Carty, Anthony Scozzari, Achim Krotz
  • Process for oligonucleotide synthesis
    Patent number: 6069243
    Abstract: The present invention describes methods for linking phosphoramidite nucleosides to a solid support-bound nucleoside. This invention presents novel processes for synthesizing oligonucleotides. Acetonitrile having a water content between 30 ppm and about 1250 ppm is used as the solvent for the washing steps in solid phase oligonucleotide synthesis, or is used as a solvent for the capping reagent or oxidizing reagent. The processes of the present invention are amenable to large-scale, economic oligonucleotide synthesis using the phosphoramidite method.
    Type: Grant
    Filed: October 6, 1998
    Date of Patent: May 30, 2000
    Assignee: Isis Pharmaceuticals, Inc.
    Inventor: Anthony Scozzari