Abstract: The present invention is directed to the use of microfluidics in the preparation of cells and compositions for therapeutic uses.

Abstract: A method for obtaining a genetically engineered leukocyte composition is provided. The method includes: (a) enriching a population of large cells from a biological sample obtained from a subject, wherein the biological sample comprises leukocytes without performing density gradient centrifugation; (b) contacting the population of large cells with an activating agent; and (c) transducing the population of large cells with a viral vector comprising a polynucleotide. The enriching comprises an array-based separation comprising a microfluidic device configured for deterministic lateral displacement comprising an array of obstacles, and the obstacles are elongated so that their length perpendicular to bulk fluid flow (P1) is longer than their width parallel to bulk fluid flow (P2) by at least 10%.

Abstract: Described herein are microfluidic devices and methods that can separate and concentrate particles in a sample.

Abstract: Described herein is a method of enriching lymphocytes from a biological sample, the method comprising: (a) removing large cells and small cells from the biological sample by a size based selection method to obtain lymphocytes, wherein the large cells comprise granulocytes or monocytes, or a combination thereof, and the small cells comprise platelets, red blood cells, or a combination thereof; (b) contacting the lymphocytes with an activating agent to obtain activated lymphocytes; and (c) removing inactivated lymphocytes from the activated lymphocytes using a sized based selection method to obtain activated enriched lymphocytes, thereby obtaining enriched activated lymphocytes.

Abstract: Described herein are systems and methods for generating a product enriched in one or more target particles, comprising a cassette to which the one or more microfluidic cartridges are releasably coupled and supported, the cassette comprising (i) a plurality input containers releasably and fluidically coupled thereto, wherein at least one of the plurality of input containers comprises an incoming sample, (ii) a plurality of outlets having a plurality of output containers releasably and fluidically coupled thereto, (iii) one or more microfluidic cartridges for separating one or more target particles from the sample, and (iv) a plurality of fluidic channels extending between the plurality of inlets, the plurality of outlets, and the one or more microfluidic cartridges, wherein the cassette having the plurality of input containers, the plurality of output containers, and the one or more microfluidic cartridges coupled thereto, collectively provides a closed end-to-end sterile environment that enables inline contin

Abstract: The present invention is directed to improved methods of preparing cells and compositions for therapeutic uses. In particular, the invention is concerned with methods which inhibit platelet activation and/or cell differentiation during the processing of blood and blood-related compositions.

Abstract: Described herein is a microfluidic cartridge for purifying target particles or target cells of a predetermined size from contaminants in a sample, the cartridge comprising a first and a second planar support the first and second planar support each having a top surface and a bottom surface, wherein the top surface of the first and/or second planar support comprises at least one embedded channel extending from one or more inlets to one or more outlets; the at least one embedded channel comprising a plurality of obstacles, wherein the microfluidic cartridge comprises at least one void space configured to be deformed when assembling the first and second planar supports into the microfluidic cartridge.

Abstract: Described herein are methods of producing enriched target cell populations that are susceptible to genetic engineering.

Abstract: Provided are methods for processing a blood related sample comprising: (a) providing a blood related sample comprising one or more target cells, platelet cells, red blood cells; and (b) reducing a number of the platelet cells in the blood related sample while maintaining a ratio of the red blood cells to the one or more target cells above a critical threshold to produce a reduced platelet blood related sample comprising the one or more target cells. Also described herein are cell compositions produced by applying the methods described herein.

Abstract: Described herein are methods of producing enriched target cell populations that are susceptible to genetic engineering.

Abstract: Described herein are methods of producing enriched target cell populations that are susceptible to genetic engineering.

Abstract: Described herein are methods of producing enriched target cell populations that are susceptible to genetic engineering.

Abstract: The present invention is directed to improved methods of preparing cells and compositions for therapeutic uses.

Abstract: The present invention is directed to the use of microfluidics in the preparation of cells and compositions for therapeutic uses.

Abstract: The present invention is directed to the use of microfluidics in the preparation of cells and compositions for therapeutic uses.

Abstract: The present invention is directed to the use of microfluidics in the preparation of cells and compositions for therapeutic uses.

Abstract: The present invention is directed to the use of microfluidics in the preparation of genetically transformed cells and compositions for therapeutic uses.

Abstract: The present invention is directed to microfluidic procedures in which cells or particles are purified with recirculation of product.

Abstract: The present invention is directed to the use of microfluidics in the preparation of cells and compositions for therapeutic uses.

Abstract: Described herein are microfluidic devices and methods that can separate and concentrate particles in a sample.