Abstract: Disclosed is a complex formed by a hybridization reaction comprising four nucleic acid molecules; the complex comprising a target nucleic acid molecule and first, second and third nucleic acid probe molecules; wherein the first probe comprises a foot region which is complementary to a first portion of the target and is hybridized thereto, and an arm region which is substantially non-complementary to the target; the second probe comprises a foot region which is complementary to a second portion of the target, such that the foot region of the second probe is hybridized to the target adjacent or substantially adjacent to the foot region of the first probe, the second probe also comprising an arm region which is substantially non-complementary to the target but which is complementary and hybridized to the arm region of the first probe; the third probe being complementary, at least in part, to a portion of the arm region of the first probe, such that third probe is hybridized to the arm region of the first probe a

Abstract: Disclosed is a method of detecting the presence of a nucleic acid target sequence of interest, the method comprising the steps of:

Abstract: Disclosed is a method of detecting a nucleic acid sequence of interest in a sample, the method comprising contacting the sample with first and second probes; the first probe comprising a portion complementary to the sequence of interest and so capable of hybridising thereto, and a portion non-complementary to the sequence of interest; the second probe comprising a portion complementary to the sequence of interest and so capable of hybridising thereto, and a portion non-complementary to the sequence of interest but complementary to that portion of the first probe which is non-complementary to the sequence of interest, such that the first and second probes are capable of hybridising to the sequence of interest in an adjacent or substantially adjacent manner, so as to allow complementary portions of the first and second probes to hybridize to each other; causing extension of the first probe with a nucleic acid polymerase, using the second probe as a template; and detecting directly or indirectly the extension of

Abstract: Disclosed is a method of detecting the presence of a nucleic acid target sequence of interest, the method comprising the steps of: (a) adding first and second nucleic acid probes to a sample comprising the sequence of interest, so as to form a complex comprising three strands of nucleic acid, wherein the first probe comprises the full length sequence of a first strand of a double stranded promoter, the target sequence comprises an end part of a second strand of the double stranded promoter which is complementary to a part of the first strand, and the second probe comprises the rest of the second strand of the double stranded promoter which is complementary to a part of the first strand, such that a functional promoter is formed when the first probe is hybridized to both the target sequence and to the second probe; (b) adding a polymerase which recognizes the promoter, so as to cause the de novo synthesis of nucleic acid from the promoter present in the complex; and (c) detecting directly or indirectly the

Abstract: A method of determining a combination of coins to be dispensed as change comprises determining a plurality of different possible combinations and selecting the most favorable one. For combinations which in sum are equal to the same amount, the most favorable combination is the one determined to leave the greatest number of coins available for change according to a predetermined criterion which takes into account the number of currently-available coins of at least one denomination. If two combinations are evaluated to be the same so far as change availability is concerned, the combination which comprises the least number of coins is selected.

Abstract: A method of determining a combination of coins to be dispensed as change comprises determining a plurality of different possible combinations and selecting the most favorable one. For combinations which in sum are equal to the same amount, the most favorable combination is the one determined to leave the greatest number of coins available for change according to a predetermined criterion which takes into account the number of currently-available coins of at least one denomination. If two combinations are evaluated to be the same so far as change availability is concerned, the combination which comprises the least number of coins is selected.