Abstract: A method for detecting at least one microorganism that may be present in a sample, comprising the steps of: a) bringing into contact, in a container: a culture medium that enables the growth and/or detection of microorganisms, said sample and a sensitized solid support; b) subjecting the whole to a temperature that promotes the growth and/or detection of microorganisms; and c) observing, in real time, the appearance of an agglutination indicating the presence of the microorganism(s) or confirming said presence when said microorganisms are detected in said culture medium, when step b) has been completed, and a method for detecting and identifying at least one target microorganism that may be present in a sample.

Abstract: A process of detecting at least one microorganism present in a sample placed in a closed container. The process includes: a) placing said sample in contact in the container with a culture medium, a revealing system capable of allowing the detection, and a support capable of capturing the microorganism(s) to be detected, the support comprising magnetic particles, b) closing the container, c) placing the container under conditions capable of allowing growth of the microorganism(s), d) applying a magnetic field to the magnetic particles for bringing together the magnetic particles, and e) detecting in real-time inside the closed container, using a detection means, the presence of the microorganism(s) fixed onto the capture support.

Abstract: The present invention generally relates to the field of analysis for example biological analysis. More specifically, the present invention relates to a process of detecting at least one microorganism present in a sample placed in a closed container, said method comprising essentially the following steps: a) Place said sample in contact in the container with at least one culture medium and a support capable of capturing the microorganism(s) to be detected, b) Close the container, c) Place the container under conditions capable of allowing the growth of the microorganism(s), d) Detect, inside said closed container, using detection means, the presence of the microorganism(s) fixed onto the capture support.

Abstract: A method of processing at least one biological sample capable of containing at least one target microorganism, the method being carried out within a container and including: (a) performing at least one step of homogenizing the biological sample, during which contents of the container are displaced from a level n, corresponding to a level of the contents at rest, to a homogenization level nh, distinct from the level n, and vice versa; and (b) generating a displacement of the contents to a level n+1, which is different from levels n and nh, such that the contents come into contact with at least one culture, at least one analysis device, or a combination thereof, positioned in a chamber of the container, between level n+1 inclusive and level nh exclusive, wherein step b) takes place before, after or during all or part of the homogenization step a).

Abstract: A method for detecting at least one microorganism present in a sample, that includes: a) in a first container, bringing the sample into contact with at least one culture medium, b) placing the first container in suitable conditions to permit growth of the microorganism or microorganisms, c) bringing some or all of the mixture being made of the sample and the culture medium into contact with a reaction mixture and a substrate for capturing the microorganism(s) in the first container or in a second container, the reaction mixture having a device for detecting the microorganism(s); d) detecting, within the first or second container, the presence of the microorganism or microorganisms detected by the detecting device and fixed on the capture substrate.

Abstract: A method of processing at least one biological sample capable of containing at least one target microorganism, corresponding analysis and enrichment methods, device enabling the implementation of said methods and the use of said device.

Abstract: One embodiment provides a device for processing at least one biological sample capable of containing at least one target microorganism within at least one container. The device having at least one displacement device for generating the displacement of the contents of the at least one container and at least one site for receiving the at least one container. Additionally, the at least one container can receive the at least one biological sample within the at least one container, the container being delimited by a wall fixed on a base. Further, the at least one displacement device may be movable with respect to the base, and the at least one container may include a flexible material which allows the at least one container to be compressed against said wall.

Abstract: The present invention relates to the field of microbiological testing of food. It relates to a kit comprising a reaction medium containing at least one inhibitor of Gram-negative bacteria and a fluorescent substrate specific for PC-PLC. It further relates to a diagnostic kit for identifying bacteria of the Bacillus cereus group comprising a container of a selective or nonselective reaction medium with a pH between 6.8 and 8.0, the medium comprising at least one inhibitor of Gram-negative bacteria and a fluorescent phosphatidylcholine phospholipase C (PC-PLC) substrate.

Abstract: The present invention relates to a method for detecting at least one target microorganism that may be present in a sample, comprising the steps of: a) bringing into contact, in a container: said sample, a medium that enables the growth of the target microorganism(s), and a cell population capable of being lysed by the target microorganism(s); b) subjecting the whole to a temperature that promotes the growth of the target microorganism(s); and c) observing, in real time, lysis of the cell population indicating the presence of the target microorganism(s). The present invention also relates to a method for detecting and identifying at least one target microorganism that may be present in a sample.

Abstract: A method of processing at least one biological sample capable of containing at least one target microorganism, corresponding analysis and enrichment methods, device enabling the implementation of said methods and the use of said device.

Abstract: The present invention relates to the field of microbiological testing of food. It relates to a method for identifying bacteria of the Bacillus cereus group, comprising the steps consisting in: bringing a sample that may contain bacteria of the Bacillus cereus group, a reaction medium comprising at least one fluorescent phosphatidylcholine phospholipase C substrate and an inhibitor of Gram-negative bacteria into contact, in a container; incubating all of the above together; identifying the bacteria of the Bacillus cereus group by detecting the PC-PLC substrate hydrolysis reaction, in which the pH of the reaction medium and the time necessary for detecting the PC-PLC substrate hydrolysis reaction carried out are adapted in such a way that said hydrolysis reaction by the bacteria of the Bacillus cereus group is detected before the hydrolysis of the PC-PLC substrate by the Gram-positive bacteria other than those belonging to the Bacillus cereus group, and potentially present in the sample, is detectable.

Abstract: The present invention relates to the field of analysis, for example biological analysis. Specifically, the invention relates to a method for detecting at least one microorganism present in a sample, the method essentially including the following steps: a) in a first container, bringing the sample into contact with at least one culture medium, b) placing the first container in suitable conditions to permit growth of the microorganism or microorganisms, c) bringing some or all of the mixture being made of the sample and the culture medium into contact with a reaction mixture and a substrate for capturing the microorganism(s) in the first container or in a second container, the reaction mixture having a device for detecting the microorganism(s); d) detecting, within the first or second container, the presence of the microorganism or microorganisms detected by the detecting device and fixed on the capture substrate.

Abstract: The present invention relates to a method for identifying bacteria of the Bacillus cereus group, comprising the following steps: (a) providing a sample that may contain bacteria of the Bacillus cereus group, a reaction medium comprising at least one fluorescent phosphatidylcholine phospholipase C (PC-PLC) substrate and an inhibitor of Gram-negative bacteria; (b) inoculating the reaction medium with the sample; (c) incubating the inoculated reaction medium; and (d) identifying the bacteria of the Bacillus cereus group by detecting the PC-PLC substrate hydrolysis reaction, in which the pH of the reaction medium and the time necessary for detecting the PC-PLC substrate hydrolysis reaction are adapted such that said hydrolysis reaction by bacteria of the Bacillus cereus group is detected before hydrolysis of the PC-PLC substrate by any Gram-positive bacteria other than those belonging to the Bacillus cereus group, that may be present in the sample.

Abstract: The present invention generally relates to the field of analysis for example biological analysis. More specifically, the present invention relates to a process of detecting at least one microorganism present in a sample placed in a closed container, said method comprising essentially the following steps: a) Place said sample in contact in the container with at least one culture medium and a support capable of capturing the microorganism(s) to be detected, b) Close the container, c) Place the container under conditions capable of allowing the growth of the microorganism(s), d) Detect, inside said closed container, using detection means, the presence of the microorganism(s) fixed onto the capture support.

Abstract: The present invention relates to the field of microbiological testing of food. It relates to a method for identifying bacteria of the Bacillus cereus group, comprising the steps consisting in: bringing a sample that may contain bacteria of the Bacillus cereus group, a reaction medium comprising at least one fluorescent phosphatidylcholine phospholipase C substrate and an inhibitor of Gram-negative bacteria into contact, in a container; incubating all of the above together; identifying the bacteria of the Bacillus cereus group by detecting the PC-PLC substrate hydrolysis reaction, in which the pH of the reaction medium and the time necessary for detecting the PC-PLC substrate hydrolysis reaction carried out are adapted in such a way that said hydrolysis reaction by the bacteria of the Bacillus cereus group is detected before the hydrolysis of the PC-PLC substrate by the Gram-positive bacteria other than those belonging to the Bacillus cereus group, and potentially present in the sample, is detectable.

Abstract: A specific culture medium for growth, detection, identification and/or counting of Staphylococcus aureus bacteria, said medium includes at least one fluorogenic, chromogenic or luminescent substrate of phospholipase C. Said medium permits differentiation between Staphylococcus aureus and coagulase-negative staphylococci.

Abstract: A method for detecting at least one microorganism that may be present in a sample, comprising the steps of: a) bringing into contact, in a container: a culture medium that enables the growth and/or detection of microorganisms, said sample and a sensitized solid support; b) subjecting the whole to a temperature that promotes the growth and/or detection of microorganisms; and c) observing, in real time, the appearance of an agglutination indicating the presence of the microorganism(s) or confirming said presence when said microorganisms are detected in said culture medium, when step b) has been completed, and a method for detecting and identifying at least one target microorganism that may be present in a sample.

Abstract: The present invention relates to a method for detecting at least one target microorganism that may be present in a sample, comprising the steps of: a) bringing into contact, in a container: said sample, a medium that enables the growth of the target microorganism(s), and a cell population capable of being lysed by the target microorganism(s); b) subjecting the whole to a temperature that promotes the growth of the target microorganism(s); and c) observing, in real time, lysis of the cell population indicating the presence of the target microorganism(s). The present invention also relates to a method for detecting and identifying at least one target microorganism that may be present in a sample.