Abstract: Disclosed herein are methods of protein quantification and normalization using haloalkylated tryptophan fluorescence. Complex protein samples, i.e., samples that each contain 1,000 or more distinct proteins, from diverse sources that do not have common protein profiles are treated with a halo-substituted organic compound (i.e. haloalkane) that reacts with tryptophan residues to form fluorescent products. Irradiation of the samples with ultraviolet light and the detection and quantification of the resultant fluorescent emissions from all proteins in each sample are then used to obtain comparative values for total protein content among the various samples.

Abstract: Disclosed herein are methods of protein quantification and normalization using haloalkylated tryptophan fluorescence. Complex protein samples, i.e., samples that each contain 1,000 or more distinct proteins, from diverse sources that do not have common protein profiles are treated with a halo-substituted organic compound (i.e. haloalkane) that reacts with tryptophan residues to form fluorescent products. Irradiation of the samples with ultraviolet light and the detection and quantification of the resultant fluorescent emissions from all proteins in each sample are then used to obtain comparative values for total protein content among the various samples.

Abstract: Disclosed herein are methods of protein quantification and normalization using haloalkylated tryptophan fluorescence. Complex protein samples, i.e., samples that each contain 1,000 or more distinct proteins, from diverse sources that do not have common protein profiles are treated with a halo-substituted organic compound (i.e. haloalkane) that reacts with tryptophan residues to form fluorescent products. Irradiation of the samples with ultraviolet light and the detection and quantification of the resultant fluorescent emissions from all proteins in each sample are then used to obtain comparative values for total protein content among the various samples.

Abstract: Methods and devices for purifying, detecting, and collecting analytes fractionated based on pI, separating analytes via electrophoresis and pI, and purifying a target molecule using pI focusing and subsequent crystallization are provided.

Abstract: Apparatuses and methods for controlling ionic strength and/or pH of a solution are provided.

Abstract: Disclosed herein are methods of protein quantification and normalization using haloalkylated tryptophan fluorescence. Complex protein samples, i.e., samples that each contain 1,000 or more distinct proteins, from diverse sources that do not have common protein profiles are treated with a halo-substituted organic compound (i.e. haloalkane) that reacts with tryptophan residues to form fluorescent products. Irradiation of the samples with ultraviolet light and the detection and quantification of the resultant fluorescent emissions from all proteins in each sample are then used to obtain comparative values for total protein content among the various samples.

Abstract: Disclosed herein are methods of protein quantification and normalization using haloalkylated tryptophan fluorescence. Complex protein samples, i.e., samples that each contain 1,000 or more distinct proteins, from diverse sources that do not have common protein profiles are treated with a halo-substituted organic compound (i.e. haloalkane) that reacts with tryptophan residues to form fluorescent products. Irradiation of the samples with ultraviolet light and the detection and quantification of the resultant fluorescent emissions from all proteins in each sample are then used to obtain comparative values for total protein content among the various samples.

Abstract: Apparatuses and methods for controlling ionic strength and/or pH of a solution are provided.

Abstract: Methods and devices for purification of different cell components from the same sample are provided.

Abstract: Methods and devices for purifying, detecting, and collecting analytes fractionated based on pI, separating analytes via electrophoresis and pI, and purifying a target molecule using pI focusing and subsequent crystallization are provided.

Abstract: Disclosed herein are methods of protein quantification and normalization using haloalkylated tryptophan fluorescence. Complex protein samples, i.e., samples that each contain 1,000 or more distinct proteins, from diverse sources that do not have common protein profiles are treated with a halo-substituted organic compound (i.e. haloalkane) that reacts with tryptophan residues to form fluorescent products. Irradiation of the samples with ultraviolet light and the detection and quantification of the resultant fluorescent emissions from all proteins in each sample are then used to obtain comparative values for total protein content among the various samples.

Abstract: Apparatuses and methods for controlling ionic strength and/or pH of a solution are provided.

Abstract: A dielectrophoretic fractionation device having a channel defining a direction of flow therethrough for the fractionation of particles in a liquid particle suspension is disclosed. The device also has a deflector unit affixed to a wall of the channel and arranged to generate an electric field gradient in the channel to spatially separate the particles in the liquid particle suspension. The device also has an actuator mounted to the channel and positioned to deform the wall. Also provided is a method for inhibiting aggregation of particles in a liquid particle suspension of the dielectrophoretic fractionation device, the method including periodically deforming the wall with the actuator.

Abstract: A gel electrophoresis cassette (33) is disclosed which includes two plates (34, 35) and at least one seal (36) which separates these plates. The seal (36) is annular; it may be positioned essentially in the region of the outer edge of the plates (34, 35). For performing an electrophoresis in a second dimension following an isoelectric focusing in an first dimension, one of the plates (34, 35) includes a recess (37) for inserting a strip holder (1) having a base plate (4), which has a carrier surface (2) on which an IEF strip (3) is accommodated. The base plate (4) includes at least one stop (5) offset to a lower level in relation to the carrier surface (2) and a sealing surface (6). The strip holder (1) is insertable into this recess (37) in such a way that the stop (5) of the base plate (4) is applied to the outer surface of the plate (34, 35) and the sealing surface (6) presses tightly against the inner surface (38) of the recess (37).

Abstract: A strip holder (1) is disclosed for accommodating a gel strip (3) for separating molecules using gel electrophoresis. The strip holder (1) disclosed is distinguished by including a baseplate (4), at least one stop (5), which is offset to a lower level in relation to the carrier surface (2), and at least one sealing surface (6), this stop (5) being implemented to be applied to counter surfaces (7) of an electrophoresis chamber, through which offset to a lower level of the stop (5) the installation depth of the strip holder (1) carrying a gel strip (3) into this electrophoresis chamber is determined and the sealing surface (6) ensuring a sealing installation of the strip holder (1) carrying a gel strip (3) into this electrophoresis chamber. Such a chamber (15) for isoelectric focusing of molecules in gel strips (3) is distinguished by including such a strip holder (1), a frame (16), and a cover (20).

Abstract: A gel electrophoresis cassette (33) is disclosed which includes two plates (34, 35) and at least one seal (36) which separates these plates. The seal (36) is annular; it may be positioned essentially in the region of the outer edge of the plates (34, 35). For performing an electrophoresis in a second dimension following an isoelectric focusing in an first dimension, one of the plates (34, 35) includes a recess (37) for inserting a strip holder (1) having a base plate (4), which has a carrier surface (2) on which an IEF strip (3) is accommodated. The base plate (4) includes at least one stop (5) offset to a lower level in relation to the carrier surface (2) and a sealing surface (6). The strip holder (1) is insertable into this recess (37) in such a way that the stop (5) of the base plate (4) is applied to the outer surface of the plate (34, 35) and the sealing surface (6) presses tightly against the inner surface (38) of the recess (37).

Abstract: A strip holder (1) is disclosed for accommodating a gel strip (3) for separating molecules using gel electrophoresis. The strip holder (1) disclosed is distinguished by including a baseplate (4), at least one stop (5), which is offset to a lower level in relation to the carrier surface (2), and at least one sealing surface (6), this stop (5) being implemented to be applied to counter surfaces (7) of an electrophoresis chamber, through which offset to a lower level of the stop (5) the installation depth of the strip holder (1) carrying a gel strip (3) into this electrophoresis chamber is determined and the sealing surface (6) ensuring a sealing installation of the strip holder (1) carrying a gel strip (3) into this electrophoresis chamber. Such a chamber (15) for isoelectric focusing of molecules in gel strips (3) is distinguished by including such a strip holder (1), a frame (16), and a cover (20).