Patents by Inventor Antonio Bernad

Antonio Bernad has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Use of beta recombinase in eukaryotic cells, especially for transgenic work
    Patent number: 6780644
    Abstract: The invention relates to the use of prokaryotic beta recombinase in eukaryotic cells, especially for transgenic work in eukaryotic cells. It also relates to the use of prokaryotic beta recombinase for site-specific intramolecular recombination between two six sites in eukaryotic cells. The use of the gene coding for beta recombinase for promoting the deletion of DNA sequences located between directly oriented six sites in mammalian cells and for catalysing site-specific resolution of DNA sequences in an extrachromosomal target introduced into an eukaryotic cell are also disclosed.
    Type: Grant
    Filed: September 22, 1998
    Date of Patent: August 24, 2004
    Assignees: Pharmacia Spain S.A., Consejo Superior de Investigaciones Cientificas
    Inventors: Vincente Días, Fernando Rojo, Carlos Martinez Alonso, Juan C. Alonso, Antonio Bernad
  • Use of beta recombinase in eukaryotic cells, especially for transgenic work
    Publication number: 20040157332
    Abstract: The invention relates to the use of prokaryotic beta recombinase in eukaryotic cells, especially for transgenic work in eukaryotic cells.
    Type: Application
    Filed: March 24, 2004
    Publication date: August 12, 2004
    Inventors: Vincente Dias, Fernando Rojo, Carlos Martinez Alonso, Juan C. Alonso, Antonio Bernad
  • .phi.29 DNA polymerase
    Patent number: 5576204
    Abstract: An improved method for determining the nucleotide base sequence of a DNA molecule employs a .phi.-29 type DNA polymerase modified to have reduced or no exonuclease activity. The method includes annealing the DNA molecule with a primer molecule able to hybridize to the DNA molecule; incubating the annealed mixture in a vessel containing four different deoxynucleoside triphosphates, a DNA polymerase, and one or more DNA synthesis terminating agents which terminate DNA synthesis at a specific nucleotide base, wherein each the agent terminates DNA synthesis at a different nucleotide base; and separating the DNA products of the incubating reaction according to size, whereby at least a part of the nucleotide base sequence of the DNA can be determined. The improvement is provision of a DNA polymerase which is a .phi.29-type DNA polymerase.
    Type: Grant
    Filed: February 11, 1993
    Date of Patent: November 19, 1996
    Assignee: Consejo Superior Investigaciones Cientificas
    Inventors: Luis Blanco, Antonio Bernad, Margarita Salas
  • PHI29 DNA polymerase
    Patent number: 5198543
    Abstract: An improved method for determining the nucleotide base sequence of a DNA molecule. The method includes annealing the DNA molecule with a primer molecule able to hybridize to the DNA molecule; incubating the annealed mixture in a vessel containing four different deoxynucleoside triphosphates, a DNA polymerase, and one or more DNA synthesis terminating agents which terminate DNA synthesis at a specific nucleotide base, wherein each the agent terminates DNA synthesis at a different nucleotide base; and separating the DNA products of the incubating reaction according to size, whereby at least a part of the nucleotide base sequence of the DNA can be determined. The improvement is provision of a DNA polymerase which is a .phi.29-type DNA polymerase.
    Type: Grant
    Filed: March 13, 1991
    Date of Patent: March 30, 1993
    Assignee: Consejo Superior Investigaciones Cientificas
    Inventors: Luis Blanco, Antonio Bernad, Margarita Salas
  • PH.phi.29 DNA polymerase
    Patent number: 5001050
    Abstract: An improved method for determining the nucleotide base sequence of a DNA molecule. The method includes annealing the DNA molecule with a primer molecule able to hybridize to the DNA molecule; incubating the annealed mixture in a vessel containing four different deoxynucleoside triphosphates, a DNA polymerase, and one or more DNA synthesis terminating agents which terminate DNA synthesis at a specific nucleotide base, wherein each the agent terminates DNA synthesis at a different nucleotide base; and separating the DNA products of the incubating reaction according to size, whereby at least a part of the nucleotide base sequence of the DNA can be determined. The improvement is provision of a DNA-polymerase which is a .phi.29-type DNA polymerase.
    Type: Grant
    Filed: March 24, 1989
    Date of Patent: March 19, 1991
    Assignee: Consejo Superior Investigaciones Cientificas
    Inventors: Luis Blanco, Antonio Bernad, Margarita Salas