Patents by Inventor Antonius Jeroen Adriaan van Maris

Antonius Jeroen Adriaan van Maris has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 11326172
    Abstract: The invention relates to a variant of a parent polypeptide which is preferably a hexose transporter, wherein the variant comprises an amino acid sequence which, when aligned with the amino acid sequence of SEQ ID NO: 1, comprises a substitution of amino acids N376 and T89, the positions of said amino acids being defined with reference to the amino acid sequence of SEQ ID NO: 1. Said polypeptide is suitable in the production of ethanol from arabinose-rich biomass such as corn stover and corn starch.
    Type: Grant
    Filed: November 12, 2018
    Date of Patent: May 10, 2022
    Assignee: DSM IP ASSETS B.V.
    Inventors: Antonius Jeroen Adriaan Van Maris, Jacobus Thomas Pronk, Maarten Dirk Verhoeven
  • Patent number: 11326175
    Abstract: The present invention relates to a yeast cell, in particular a recombinant yeast cell, the cell lacking enzymatic activity needed for the NADH-dependent glycerol synthesis or the cell having a reduced enzymatic activity with respect to the NADH-dependent glycerol synthesis compared to its corresponding wild-type yeast cell, the cell comprising one or more heterologous nucleic acid sequences encoding an NAD+-dependent acetylating acetaldehyde dehydrogenase (EC 1.2.1.10) activity. The invention further relates to the use of a cell according to the invention in the preparation of ethanol.
    Type: Grant
    Filed: February 15, 2021
    Date of Patent: May 10, 2022
    Assignee: DSM IP ASSETS B.V.
    Inventors: Jacobus Thomas Pronk, Antonius Jeroen Adriaan Van Maris, Victor Gabriel Guadalupe Medina
  • Publication number: 20220135989
    Abstract: The present invention relates to a yeast cell, in particular a recombinant yeast cell, the cell lacking enzymatic activity needed for the NADH-dependent glycerol synthesis or the cell having a reduced enzymatic activity with respect to the NADH-dependent glycerol synthesis compared to its corresponding wild-type yeast cell, the cell comprising one or more heterologous nucleic acid sequences encoding an NAD+-dependent acetylating acetaldehyde dehydrogenase (EC 1.2.1.10) activity. The invention further relates to the use of a cell according to the invention in the preparation of ethanol.
    Type: Application
    Filed: November 19, 2021
    Publication date: May 5, 2022
    Inventors: Jacobus Thomas PRONK, Antonius Jeroen Adriaan VAN MARIS, Victor Gabriel GUADALUPE MEDINA
  • Patent number: 11214810
    Abstract: The present invention relates to a yeast cell, in particular a recombinant yeast cell, the cell lacking enzymatic activity needed for the NADH-dependent glycerol synthesis or the cell having a reduced enzymatic activity with respect to the NADH-dependent glycerol synthesis compared to its corresponding wild-type yeast cell, the cell comprising one or more heterologous nucleic acid sequences encoding an NAD+-dependent acetylating acetaldehyde dehydrogenase (EC 1.2.1.10) activity. The invention further relates to the use of a cell according to the invention in the preparation of ethanol.
    Type: Grant
    Filed: March 27, 2020
    Date of Patent: January 4, 2022
    Assignee: DSM IP ASSETS B.V.
    Inventors: Jacobus Thomas Pronk, Antonius Jeroen Adriaan Van Maris, Victor Gabriel Guadalupe Medina
  • Patent number: 11198847
    Abstract: The invention relates to a method for preparing a yeast which is capable of simultaneously fermenting a pentose and a hexose sugar, the method comprising providing a yeast which comprises: one or more heterologous genes encoding an enzyme of a pentose metabolic pathway, a disruption of a gene encoding a ribulose-phosphate 3-epimerase and a disruption of a gene encoding a glucose-6-phosphate isomerase, and one or more overexpressed endogenous genes encoding an enzyme of the pentose phosphate pathway; and subjecting the yeast to evolutionary engineering on a medium comprising a hexose sugar and at least one pentose sugar, selecting for a yeast with improved growth rate when grown on a media comprising a hexose and at least one pentose sugar, so as to obtain an evolved yeast.
    Type: Grant
    Filed: January 31, 2019
    Date of Patent: December 14, 2021
    Assignee: DSM IP ASSETS B.V.
    Inventors: Antonius Jeroen Adriaan Van Maris, Jacobus Thomas Pronk, Maarten Dirk Verhoeven, Ioannis Papapetridis
  • Patent number: 11186850
    Abstract: The present invention describes a recombinant yeast cell functionally expressing one or more heterologous nucleic acid sequences encoding for ribulose-1,5-phosphate carboxylase/oxygenase (EC4.1.1.39; Rubisco), and optionally one or more molecular chaperones for Rubisco, and one or more phosphoribulokinase (EC2.7.1.19; PRK), wherein the phosphoribulokinase is under control of a promoter (the “PRK promoter”) that enables higher expression under anaerobic conditions than under aerobic conditions.
    Type: Grant
    Filed: April 24, 2020
    Date of Patent: November 30, 2021
    Assignee: DSM IP ASSETS B.V.
    Inventors: Ioannis Papapetridis, Antonius Jeroen Adriaan Van Maris, Jacobus Thomas Pronk, Paulus Petrus De Waal, Niels Lauret, Maarten Dirk Verhoeven, Jozef Petrus Johannes Schmitz
  • Patent number: 11174489
    Abstract: The present invention relates to a yeast cell, in particular a recombinant yeast cell, the cell lacking enzymatic activity needed for the NADH-dependent glycerol synthesis or the cell having a reduced enzymatic activity with respect to the NADH-dependent glycerol synthesis compared to its corresponding wild-type yeast cell, the cell comprising one or more heterologous nucleic acid sequences encoding an NAD+-dependent acetylating acetaldehyde dehydrogenase (EC 1.2.1.10) activity. The invention further relates to the use of a cell according to the invention in the preparation of ethanol.
    Type: Grant
    Filed: February 15, 2021
    Date of Patent: November 16, 2021
    Assignee: DSM IP ASSETS B.V.
    Inventors: Jacobus Thomas Pronk, Antonius Jeroen Adriaan Van Maris, Victor Gabriel Guadalupe Medina
  • Patent number: 11136600
    Abstract: The present invention relates to a eukaryotic cell that is genetically modified comprising one or more heterologous gene encoding: a) D-glucose-6-phosphate dehydrogenase and/or b) 6-phosphogluconate dehydrogenase; and/or c) glucose dehydrogenase, gluconolactonase and gluconate kinase, wherein a), b) and glucose dehydrogenase in c) are NAD+ dependent.
    Type: Grant
    Filed: October 3, 2016
    Date of Patent: October 5, 2021
    Assignee: DSM IP Assets B.V.
    Inventors: Ioannis Papapetridis, Jacobus Thomas Pronk, Antonius Jeroen Adriaan Van Maris, Paul Klaassen
  • Publication number: 20210230613
    Abstract: The present invention relates to a yeast cell, in particular a recombinant yeast cell, the cell lacking enzymatic activity needed for the NADH-dependent glycerol synthesis or the cell having a reduced enzymatic activity with respect to the NADH-dependent glycerol synthesis compared to its corresponding wild-type yeast cell, the cell comprising one or more heterologous nucleic acid sequences encoding an NAD+-dependent acetylating acetaldehyde dehydrogenase (EC 1.2.1.10) activity. The invention further relates to the use of a cell according to the invention in the preparation of ethanol.
    Type: Application
    Filed: February 15, 2021
    Publication date: July 29, 2021
    Inventors: Jacobus Thomas PRONK, Antonius Jeroen Adriaan VAN MARIS, Victor Gabriel GUADALUPE MEDINA
  • Publication number: 20210230614
    Abstract: The present invention relates to a yeast cell, in particular a recombinant yeast cell, the cell lacking enzymatic activity needed for the NADH-dependent glycerol synthesis or the cell having a reduced enzymatic activity with respect to the NADH-dependent glycerol synthesis compared to its corresponding wild-type yeast cell, the cell comprising one or more heterologous nucleic acid sequences encoding an NAD+-dependent acetylating acetaldehyde dehydrogenase (EC 1.2.1.10) activity. The invention further relates to the use of a cell according to the invention in the preparation of ethanol.
    Type: Application
    Filed: February 15, 2021
    Publication date: July 29, 2021
    Inventors: Jacobus Thomas PRONK, Antonius Jeroen Adriaan VAN MARIS, Victor Gabriel GUADALUPE MEDINA
  • Publication number: 20210147859
    Abstract: The invention relates to a recombinant yeast cell, in particular a transgenic yeast cell, functionally expressing one or more recombinant, in particular heterologous, nucleic acid sequences encoding ribulose-1,5-biphosphate carboxylase oxygenase (Rubisco) and phosphoribulokinase (PRK). The invention further relates to the use of carbon dioxide as an electron acceptor in a recombinant chemotrophic micro-organism, in particular a eukaryotic micro-organism.
    Type: Application
    Filed: December 1, 2020
    Publication date: May 20, 2021
    Inventors: Antonius Jeroen Adriaan VAN MARIS, Jacobus Thomas PRONK, Victor Gabriel GUADALUPE MEDINA, Hendrik Wouter WISSELINK
  • Patent number: 10913928
    Abstract: This invention relates to a recombinant cell, preferably a recombinant yeast cell comprising: a) a gene coding for an enzyme having glycerol-3-phosphate dehydrogenase activity, wherein said enzyme has a cofactor dependency for at least NADP+ and/or for NADPH; b) a gene encoding an enzyme having at least NAD+ dependent acetylating acetaldehyde dehydrogenase activity (EC 1.2.1.10); and c) a mutation or disruption in at least one gene selected from the group of GPD1 and GPD2. Said cell is suitable for ethanol production, has a reduced glycerol production at high ethanol yield.
    Type: Grant
    Filed: December 18, 2017
    Date of Patent: February 9, 2021
    Assignee: DSM IP ASSETS B.V.
    Inventors: Ioannis Papapetridis, Antonius Jeroen Adriaan Van Maris, Jacobus Thomas Pronk
  • Publication number: 20210002651
    Abstract: The invention relates to a variant of a parent polypeptide which is preferably a hexose transporter, wherein the variant comprises an amino acid sequence which, when aligned with the amino acid sequence of SEQ ID NO: 1, comprises a substitution of amino acids N376 and T89, the positions of said amino acids being defined with reference to the amino acid sequence of SEQ ID NO: 1. Said polypeptide is suitable in the production of ethanol from arabinose-rich biomass such as corn stover and corn starch.
    Type: Application
    Filed: November 12, 2018
    Publication date: January 7, 2021
    Inventors: Antonius Jeroen Adriaan VAN MARIS, Jacobus Thomas PRONK, Maarten Dirk VERHOEVEN
  • Patent number: 10883110
    Abstract: The present invention relates to a yeast cell, in particular a recombinant yeast cell, the cell lacking enzymatic activity needed for the NADH-dependent glycerol synthesis or the cell having a reduced enzymatic activity with respect to the NADH-dependent glycerol synthesis compared to its corresponding wild-type yeast cell, the cell comprising one or more heterologous nucleic acid sequences encoding an NAD+-dependent acetylating acetaldehyde dehydrogenase (EC 1.2.1.10) activity. The invention further relates to the use of a cell according to the invention in the preparation of ethanol.
    Type: Grant
    Filed: June 4, 2020
    Date of Patent: January 5, 2021
    Assignee: DSM IP ASSETS B.V.
    Inventors: Jacobus Thomas Pronk, Antonius Jeroen Adriaan Van Maris, Victor Gabriel Guadalupe Medina
  • Publication number: 20200377846
    Abstract: A method for preparing a yeast capable of simultaneously fermenting a pentose and a hexose sugar, comprising: (a) providing a yeast comprising: one or more heterologous genes encoding an enzyme of a pentose metabolic pathway; disruptions of a gene encoding a ribulose-phosphate 3-epimerase and of a gene encoding a glucose-6-phosphate isomerase; one or more overexpressed endogenous genes encoding an enzyme of the pentose phosphate pathway; and a disruption of one or more genes encoding an NADPH dependent 6-phosphogluconate dehydrogenase, (b) subjecting said yeast to evolutionary engineering on a medium comprising a hexose sugar and at least one pentose sugar, selecting for a yeast with improved growth rate obtain an evolved yeast; (d) restoring, in the evolved yeast, one or more of the disrupted genes, or: (d?) identifying genetic permutations in at least part of the genome of the evolved yeast by genome sequencing; (e) constructing an improved pentose and hexose-fermenting yeast comprising one or more said gen
    Type: Application
    Filed: January 31, 2019
    Publication date: December 3, 2020
    Inventors: Antonius Jeroen Adriaan VAN MARIS, Jacobus Thomas PRONK, Maarten Dirk VERHOEVEN, Ioannis PAPAPETRIDIS
  • Publication number: 20200299704
    Abstract: The present invention relates to a yeast cell, in particular a recombinant yeast cell, the cell lacking enzymatic activity needed for the NADH-dependent glycerol synthesis or the cell having a reduced enzymatic activity with respect to the NADH-dependent glycerol synthesis compared to its corresponding wild-type yeast cell, the cell comprising one or more heterologous nucleic acid sequences encoding an NAD+-dependent acetylating acetaldehyde dehydrogenase (EC 1.2.1.10) activity. The invention further relates to the use of a cell according to the invention in the preparation of ethanol.
    Type: Application
    Filed: June 4, 2020
    Publication date: September 24, 2020
    Inventors: Jacobus Thomas PRONK, Antonius Jeroen Adriaan VAN MARIS, Victor Gabriel GUADALUPE MEDINA
  • Publication number: 20200255871
    Abstract: The present invention describes a recombinant yeast cell functionally expressing one or more heterologous nucleic acid sequences encoding for ribulose-1,5-phosphate carboxylase/oxygenase (EC4.1.1.39; Rubisco), and optionally one or more molecular chaperones for Rubisco, and one or more phosphoribulokinase (EC2.7.1.19; PRK), wherein the phosphoribulokinase is under control of a promoter (the “PRK promoter”) that enables higher expression under anaerobic conditions than under aerobic conditions.
    Type: Application
    Filed: April 24, 2020
    Publication date: August 13, 2020
    Inventors: Ioannis PAPAPETRIDIS, Antonius Jeroen Adriaan VAN MARIS, Jacobus Thomas PRONK, Paulus Petrus DE WAAL, Niels LAURET, Maarten Dirk VERHOEVEN, Jozef Petrus Johannes SCHMITZ
  • Patent number: 10738317
    Abstract: The present invention relates to a yeast cell, in particular a recombinant yeast cell, the cell lacking enzymatic activity needed for the NADH-dependent glycerol synthesis or the cell having a reduced enzymatic activity with respect to the NADH-dependent glycerol synthesis compared to its corresponding wild-type yeast cell, the cell comprising one or more heterologous nucleic acid sequences encoding an NAD+-dependent acetylating acetaldehyde dehydrogenase (EC 1.2.1.10) activity. The invention further relates to the use of a cell according to the invention in the preparation of ethanol.
    Type: Grant
    Filed: May 3, 2018
    Date of Patent: August 11, 2020
    Assignee: DSM IP ASSETS B.V.
    Inventors: Jacobus Thomas Pronk, Antonius Jeroen Adriaan Van Maris, Victor Gabriel Guadalupe Medina
  • Publication number: 20200248194
    Abstract: The present invention relates to a yeast cell, in particular a recombinant yeast cell, the cell lacking enzymatic activity needed for the NADH-dependent glycerol synthesis or the cell having a reduced enzymatic activity with respect to the NADH-dependent glycerol synthesis compared to its corresponding wild-type yeast cell, the cell comprising one or more heterologous nucleic acid sequences encoding an NAD+-dependent acetylating acetaldehyde dehydrogenase (EC 1.2.1.10) activity. The invention further relates to the use of a cell according to the invention in the preparation of ethanol.
    Type: Application
    Filed: March 27, 2020
    Publication date: August 6, 2020
    Inventors: Jacobus Thomas PRONK, Antonius Jeroen Adriaan VAN MARIS, Victor Gabriel GUADALUPE MEDINA
  • Patent number: 10689670
    Abstract: The present invention describes a recombinant yeast cell functionally expressing one or more heterologous nucleic acid sequences encoding for ribulose-1,5-phosphate carboxylase/oxygenase (EC4.1.1.39; Rubisco), and optionally one or more molecular chaperones for Rubisco, and one or more phosphoribulokinase (EC2.7.1.19; PRK), wherein the phosphoribulokinase is under control of a promoter (the “PRK promoter”) that enables higher expression under anaerobic conditions than under aerobic conditions.
    Type: Grant
    Filed: June 13, 2017
    Date of Patent: June 23, 2020
    Assignee: DSM IP ASSETS B.V.
    Inventors: Ioannis Papapetridis, Antonius Jeroen Adriaan Van Maris, Jacobus Thomas Pronk, Paulus Petrus De Waal, Niels Lauret, Maarten Dirk Verhoeven, Jozef Petrus Johannes Schmitz