Abstract: The present invention relates to a laser scanning microscope capable of acquiring suitable fluorescent images. A fluorescence detection unit 26 measures a plurality of intensities of fluorescent light of different wavelengths that is excited by irradiation of laser light and emitted from a sample 12.

Abstract: The present invention relates to a laser scanning microscope capable of acquiring suitable fluorescent images. A fluorescence detection unit 26 measures a plurality of intensities of fluorescent light of different wavelengths that is excited by irradiation of laser light and emitted from a sample 12.

Abstract: A fluorescence detection unit measures light quality of fluorescence from a sample excited by irradiation of a laser beam, and a transmitted light detection unit measures light quantity of the laser beam transmitted through the sample. A CLEM processing unit stops irradiation of the laser beam at every one pixel correspondence range scanning time, in one of a case where light quality of fluorescence at a lower limit value determination time is less than a lower limit threshold, and a case where light quantity of fluorescence is an upper limit threshold or more during the one pixel correspondence range scanning time. The CLEM processing unit extrapolates light quantity of the transmitted light that is supposed to be measured if exposure is performed for the one pixel correspondence range scanning time, and an image obtaining unit obtains a transmitted light image based on the extrapolated light quantity.