Patents by Inventor Anup Sood

Anup Sood has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 10294454
    Abstract: Provided are methods and kits for activating T cells, the method comprising providing a population of T cells, adding a plurality of first agents, where the first agent comprises a T-cell activator and a first binder moiety, and adding a second agent comprising a plurality of capture oligomers, where at least a segment of at least one of the plurality of capture oligomers is capable of associating with the first binder moiety. The method further comprises incubating the population of T cells, whereby at least a portion of the population of T cells is activated.
    Type: Grant
    Filed: August 24, 2016
    Date of Patent: May 21, 2019
    Assignee: General Electric Company
    Inventors: Ernest William Kovacs, Anup Sood, Reginald Donovan Smith, Evelina Roxana Loghin, Padmaparna Chadhuri, Vandana Keskar, Chrystal Mae Chadwick, Martin James Brown
  • Publication number: 20190080146
    Abstract: The subject matter of the present disclosure generally relates to techniques for image analysis. In certain embodiments, various morphological or intensity-based features as well as different thresholding approaches may be used to segment the subpopulation of interest and classify object in the images.
    Type: Application
    Filed: September 13, 2017
    Publication date: March 14, 2019
    Inventors: Alberto Santamaria-Pang, Qing Li, Yunxia Sui, Dmitry Vladimirovich Dylov, Christopher James Sevinsky, Michael E. Marino, Michael J. Gerdes, Daniel Eugene Meyer, Fiona Ginty, Anup Sood
  • Publication number: 20190060347
    Abstract: The present invention relates to a microbubble complex comprising a microbubble having an outer shell comprising a mixture of native and denatured albumin encapsulating a perfluorocarbon gas, a therapeutic agent, a bifunctional linker having one end attached to the therapeutic agent and the other attached to a ligand and wherein the ligand is bound to the other shell of the microbubble through hydrophobic interactions. Also included are methods for delivering the aforementioned microbubble complex to a tissue target.
    Type: Application
    Filed: June 25, 2018
    Publication date: February 28, 2019
    Inventors: Anup Sood, Lisa Anne Lowery, Praveena Mohan, James Edward Rothman, John Donald Burczak, Hae Won Lim
  • Patent number: 10175174
    Abstract: Methods comprising the use of photoactivated chemical bleaching for detecting multiple targets in a biological sample are provided. The methods include the steps of providing a biological sample including multiple targets, binding at least one probe to one or more target present in the sample, and detecting a signal from the probe. The method further includes the steps of contacting the sample comprising the bound probe with an electron transfer reagent, as well as an optional additive which prevents target modification during photoactivated chemical bleaching, and irradiating the sample, thereby initiating a photoreaction that substantially inactivates the probe by photoactivated chemical bleaching. The method further includes the steps of binding at least one probe to one or more target present in the sample, and detecting a signal from the probe. The process of binding, defecting and bleaching may be iteratively repeated.
    Type: Grant
    Filed: December 11, 2013
    Date of Patent: January 8, 2019
    Assignee: GE HEALTHCARE BIO-SCIENCES CORP.
    Inventors: Arunkumar Natarajan, Robert John Filkins, Anup Sood, Lakshmi Sireesha Kaanumalle, Kashan Ali Shaikh, Christina Lowes
  • Patent number: 10101322
    Abstract: Methods comprising the use of photoactivated chemical bleaching for detecting multiple targets in a biological sample are provided. The methods include the steps of providing a biological sample containing multiple targets, binding at least one probe to one or more target present in the sample, and observing a signal from the probe. The method further includes the steps of contacting the sample comprising the bound probe with a cationic or zwitterionic borate compound and irradiating the sample, thereby initiating a photoreaction that substantially inactivates the probe by photoactivated chemical bleaching. The method further includes the steps of binding at least one probe to one or more target present in the sample, and observing a signal from the probe. The process of binding, observing and bleaching may be iteratively repeated.
    Type: Grant
    Filed: February 13, 2015
    Date of Patent: October 16, 2018
    Assignee: GENERAL ELECTRIC COMPANY
    Inventors: Arunkumar Natarajan, Anup Sood, Lakshmi Sireesha Kaanumalle, Christina Lowes
  • Patent number: 10019796
    Abstract: Disclosed are novel computer-implemented methods for creating a blood vessel map of a biological tissue. The methods comprise the steps of, accessing image data corresponding to multi-channel multiplexed image of a fluorescently stained biological tissue manifesting expression levels of a primary marker and at least one auxiliary marker of blood vasculature, and extracting features of blood vessels using the primary marker as an input to create a single channel segmentation of the blood vessels. The method further comprises the steps of extracting features of blood vessels using the auxiliary marker to create auxiliary channels as a second input and apply multi-channel blood vessel enhancement. Blood vessel maps are created using the features and tracing the blood vasculature by iteratively extending the centerlines of the initial segmentation using statistical models and geometric rules.
    Type: Grant
    Filed: September 20, 2016
    Date of Patent: July 10, 2018
    Assignee: GENERAL ELECTRIC COMPANY
    Inventors: Yousef Al-Kofahi, Anup Sood, Fiona Ginty, Qing Li, Christopher James Sevinsky, Alberto Santamaria-Pang
  • Patent number: 10004760
    Abstract: The present invention relates to a microbubble complex comprising a microbubble having an outer shell comprising a mixture of native and denatured albumin encapsulating a perfluorocarbon gas, a therapeutic agent, a bifunctional linker having one end attached to the therapeutic agent and the other attached to a ligand and wherein the ligand is bound to the other shell of the microbubble through hydrophobic interactions. Also included are methods for delivering the aforementioned microbubble complex to a tissue target.
    Type: Grant
    Filed: July 26, 2016
    Date of Patent: June 26, 2018
    Assignee: GENERAL ELECTRIC COMPANY
    Inventors: Praveena Mohan, Anup Sood, James Edward Rothman, John Donald Burczak, Hae Won Lim, Lisa Anne Lowery
  • Patent number: 9984199
    Abstract: The disclosed embodiments are directed to a method for accurately counting and characterizing multiple cell phenotypes and sub-phenotypes within cell populations simultaneously by exploiting biomarker co-expression levels within cells of different phenotypes in the same tissue sample. The disclosed embodiments are also directed to a simple intuitive interface enabling medical staff (e.g., pathologists, biologists) to annotate and evaluate different cell phenotypes used in the algorithm and the presented through the interface.
    Type: Grant
    Filed: May 21, 2015
    Date of Patent: May 29, 2018
    Assignee: GE HEALTHCARE BIO-SCIENCES CORP.
    Inventors: Anup Sood, Fiona Ginty, Nicole Evelyn LaPlante, Christopher James Sevinsky, Qing Li, Alberto Santamaria-Pang, Raghav Krishna Padmanabhan
  • Patent number: 9915592
    Abstract: Methods comprising probing multiple targets in an H&E stained biological sample are provided. The methods include the steps of providing a hematoxylin and eosin stained biological sample containing multiple targets, optionally detecting H&E staining of the sample, removing the hematoxylin and eosin signals, and detecting additional features or targets in the biological sample. The detecting step may include performing the steps of binding at least one probe to one or more targets to the sample, detecting a signal from the probe and contacting the sample with a bleaching agent. The process of binding, detecting and bleaching may be iteratively repeated.
    Type: Grant
    Filed: March 5, 2014
    Date of Patent: March 13, 2018
    Assignee: General Electric Company
    Inventors: Anup Sood, Kevin Bernard Kenny, Arunkumar Natarajan, Lakshmi Sireesha Kaanumalle, Elizabeth Mary McDonough
  • Publication number: 20180057791
    Abstract: Provided are methods and kits for activating T cells, the method comprising providing a population of T cells, adding a plurality of first agents, where the first agent comprises a T-cell activator and a first binder moiety, and adding a second agent comprising a plurality of capture oligomers, where at least a segment of at least one of the plurality of capture oligomers is capable of associating with the first binder moiety. The method further comprises incubating the population of T cells, whereby at least a portion of the population of T cells is activated.
    Type: Application
    Filed: August 24, 2016
    Publication date: March 1, 2018
    Inventors: Ernest William Kovacs, Anup Sood, Reginald Donovan Smith, Evelina Roxana Loghin, Padmaparna Chadhuri, Vandana Keskar, Chrystal Mae Chadwick, Martin James Brown
  • Patent number: 9708349
    Abstract: A borate compound for use as photoinduced chemical bleaching reagent is disclosed having improved bleaching performance under certain conditions, compared to neutral borates. The compounds are comprised of Formula I; R1R2R3R4BXa?? I where each R1, R2, and R3 and R4 is, independently, an alkyl, alkylaryl, or arylalkyl, with the proviso that at least one of R1, R2, and R3 and R4 is an alkyl group and at least two of R1, R2, R3 and R4 group is positively charged; X is an anion; and a equals 1, 2, or 3.
    Type: Grant
    Filed: February 13, 2015
    Date of Patent: July 18, 2017
    Assignee: GENERAL ELECTRIC COMPANY
    Inventors: Arunkumar Natarajan, Anup Sood, Lakshmi Sireesha Kaanumalle
  • Patent number: 9677125
    Abstract: Methods for detecting a plurality of targets in a biological sample are provided. The method comprises contacting the biological sample with a plurality of target-binding probes to form a plurality of target-bound probes, covalently attaching at least one of the target-bound probes to the biological sample, and observing the signals from the target-bound probes sequentially. An associated kit and device for detection of the plurality of targets are also provided.
    Type: Grant
    Filed: October 21, 2009
    Date of Patent: June 13, 2017
    Assignee: General Electric Company
    Inventors: Anup Sood, John Richard Nelson, Michael John Gerdes
  • Publication number: 20170122872
    Abstract: The invention discloses a method for reduction of autofluorescence in biological samples, comprising the steps of: a) providing a biological microscopy sample; b) irradiating the sample with visible light, wherein the visible light has a spectrum such that at least 50% of the light intensity emanates from a narrow wavelength interval within the visible range. The invention also discloses a method for autofluorescence reduction with triplet sensitizers irradiated with visible light.
    Type: Application
    Filed: October 20, 2016
    Publication date: May 4, 2017
    Inventors: Anup Sood, ARUNKUMAR NATARAJAN, Dmitry Dylov, Lakshmi Sireesha Kaanumalle, Elizabeth Mary McDonough
  • Publication number: 20170109880
    Abstract: Disclosed are novel computer-implemented methods for creating a blood vessel map of a biological tissue. The methods comprise the steps of, accessing image data corresponding to multi-channel multiplexed image of a fluorescently stained biological tissue manifesting expression levels of a primary marker and at least one auxiliary marker of blood vasculature, and extracting features of blood vessels using the primary marker as an input to create a single channel segmentation of the blood vessels. The method further comprises the steps of extracting features of blood vessels using the auxiliary marker to create auxiliary channels as a second input and apply multi-channel blood vessel enhancement.
    Type: Application
    Filed: September 20, 2016
    Publication date: April 20, 2017
    Inventors: Yousef Al-Kofahi, Anup Sood, Fiona Ginty, Qing Li, Christopher James Sevinsky, Alberto Santamaria-Pang
  • Patent number: 9607375
    Abstract: Dynamic linking of pathway maps and cell maps is disclosed in certain embodiments. In such embodiments, the pathway maps are linked to spatially-localized regional nucleic acid data (e.g., sequence data), as opposed to non-spatially selected nucleic acid data. The pathway map and cell map data may be linked so that interactions results in changes or updates to the linked map, such as the selection or highlighting of cells exhibiting pathway map characteristics specified by a user of updating of node values or states to correspond to that of a cell or cells selected by the user.
    Type: Grant
    Filed: June 3, 2015
    Date of Patent: March 28, 2017
    Inventors: Brion Daryl Sarachan, John Frederick Graf, Anup Sood, Vidya Pundalik Kamath
  • Publication number: 20170007636
    Abstract: The present invention relates to a microbubble complex comprising a microbubble having an outer shell comprising a mixture of native and denatured albumin encapsulating a perfluorocarbon gas, a therapeutic agent, a bifunctional linker having one end attached to the therapeutic agent and the other attached to a ligand and wherein the ligand is bound to the other shell of the microbubble through hydrophobic interactions. Also included are methods for delivering the aforementioned microbubble complex to a tissue target.
    Type: Application
    Filed: July 26, 2016
    Publication date: January 12, 2017
    Inventors: Praveena Mohan, Anup Sood, James Edward Rothman, John Donald Burczak, Hae Won Lim, Lisa Anne Lowery
  • Patent number: 9518982
    Abstract: Methods for detecting multiple targets in a biological sample are provided. The methods includes contacting the sample with a first probe; physically binding the first probe to a first target; observing a first signal from the first probe; applying a chemical agent to modify the first signal; contacting the sample with a second probe; physically binding the second probe to a second target; and observing a second signal from the second probe. The methods disclosed herein also provide for multiple iterations of binding, observing, signal modification for deriving information about multiple targets in a single sample. An associated kit and device are also provided.
    Type: Grant
    Filed: November 3, 2009
    Date of Patent: December 13, 2016
    Assignee: GENERAL ELECTRIC COMPANY
    Inventors: Anup Sood, Michael Christopher Montalto, Michael Gerdes, Maximilian Lewis Seel, Robert John Filkins
  • Publication number: 20160358326
    Abstract: Dynamic linking of pathway maps and cell maps is disclosed in certain embodiments. In such embodiments, the pathway maps are linked to spatially-localized regional nucleic acid data (e.g., sequence data), as opposed to non-spatially selected nucleic acid data. The pathway map and cell map data may be linked so that interactions results in changes or updates to the linked map, such as the selection or highlighting of cells exhibiting pathway map characteristics specified by a user of updating of node values or states to correspond to that of a cell or cells selected by the user.
    Type: Application
    Filed: June 3, 2015
    Publication date: December 8, 2016
    Inventors: Brion Daryl Sarachan, John Frederick Graf, Anup Sood, Vidya Pundalik Kamath
  • Publication number: 20160341731
    Abstract: The disclosed embodiments are directed to a method for accurately counting and characterizing multiple cell phenotypes and sub-phenotypes within cell populations simultaneously by exploiting biomarker co-expression levels within cells of different phenotypes in the same tissue sample. The disclosed embodiments are also directed to a simple intuitive interface enabling medical staff (e.g., pathologists, biologists) to annotate and evaluate different cell phenotypes used in the algorithm and the presented through the interface.
    Type: Application
    Filed: May 21, 2015
    Publication date: November 24, 2016
    Inventors: Anup Sood, Fiona Ginty, Nicole Evelyn LaPlante, Christopher James Sevinsky, Qing Li, Alberto Santamaria-Pang, Raghav Krishna Padmanabhan
  • Publication number: 20160238592
    Abstract: Methods comprising the use of photoactivated chemical bleaching for detecting multiple targets in a biological sample are provided. The methods include the steps of providing a biological sample containing multiple targets, binding at least one probe to one or more target present in the sample, and observing a signal from the probe. The method further includes the steps of contacting the sample comprising the bound probe with a cationic or zwitterionic borate compound and irradiating the sample, thereby initiating a photoreaction that substantially inactivates the probe by photoactivated chemical bleaching. The method further includes the steps of binding at least one probe to one or more target present in the sample, and observing a signal from the probe. The process of binding, observing and bleaching may be iteratively repeated.
    Type: Application
    Filed: February 13, 2015
    Publication date: August 18, 2016
    Inventors: Arunkumar Natarajan, Anup Sood, Lakshmi Sireesha Kaanumalle, Christina Lowes