Abstract: Microfluidic devices and methods for perfusing a cell with perfusion fluid are provided herein, wherein the gravitational forces acting on the cell to keep the cell at or near a retainer or a retaining position exceed the hydrodynamic forces acting on the cell to move it toward an outlet.

Abstract: Microfluidic devices are provided for trapping, isolating, and processing single cells. The microfluidic devices include a cell capture chamber having a cell funnel positioned within the cell capture chamber to direct a cell passing through the cell capture chamber towards one or more a cell traps positioned downstream of the funnel to receive a cell flowing. The devices may further include auxiliary chambers integrated with the cell capture chamber for subsequent processing and assaying of the contents of a captured cell. Methods for cell capture and preparation are also provided that include flowing cells through a chamber, funneling the cells towards a cell trap, capturing a predefined number of the cells within the trap, interrupting the flow of cells, flowing a wash solution through the chamber to remove contaminants from the chamber, and sealing the predefined number of cells in the chamber.

Abstract: Microfluidic devices and methods for perfusing a cell with perfusion fluid are provided herein, wherein the gravitational forces acting on the cell to keep the cell at or near a retainer or a retaining position exceed the hydrodynamic forces acting on the cell to move it toward an outlet. Also provided, are methods for assaying cell products within the microfluidic device.

Abstract: Microfluidic devices are provided for trapping, isolating, and processing single cells. The microfluidic devices include a cell capture chamber having a cell funnel positioned within the cell capture chamber to direct a cell passing through the cell capture chamber towards one or more a cell traps positioned downstream of the funnel to receive a cell flowing. The devices may further include auxiliary chambers integrated with the cell capture chamber for subsequent processing and assaying of the contents of a captured cell. Methods for cell capture and preparation are also provided that include flowing cells through a chamber, funneling the cells towards a cell trap, capturing a predefined number of the cells within the trap, interrupting the flow of cells, flowing a wash solution through the chamber to remove contaminants from the chamber, and sealing the predefined number of cells in the chamber.

Abstract: Methods and devices are provided herein for identifying a cell population comprising an effector cell that exerts an extracellular effect. In one embodiment the method comprises retaining in a microreactor a cell population comprising one or more effector cells, wherein the contents of the microreactor further comprise a readout particle population comprising one or more readout particles, incubating the cell population and the readout particle population within the microreactor, assaying the cell population for the presence of the extracellular effect, wherein the readout particle population or subpopulation thereof provides a direct or indirect readout of the extracellular effect, and determining, based on the results of the assaying step, whether one or more effector cells within the cell population exerts the extracellular effect on the readout particle. If an extracellular effect is measured, the cell population is recovered for further analysis to determine the cell or cells responsible for the effect.

Abstract: There are provided methods, and devices for assaying for a binding interaction between a protein, such as a monoclonal antibody, produced by a cell, and a biomolecule. The method may include retaining the cell within a chamber having an aperture; exposing the protein produced by the cell to a capture substrate, wherein the capture substrate is in fluid communication with the protein produced by the cell and wherein the capture substrate is operable to bind the protein produced by the cell; flowing a fluid volume comprising the biomolecule through the chamber via said aperture, wherein the fluid volume is in fluid communication with the capture substrate; and determining a binding interaction between the protein produced by the cell and the biomolecule.

Abstract: There are provided methods, and devices for assaying for a binding interaction between a protein, such as a monoclonal antibody, produced by a cell, and a biomolecule. The method may include retaining the cell within a chamber having an aperture; exposing the protein produced by the cell to a capture substrate, wherein the capture substrate is in fluid communication with the protein produced by the cell and wherein the capture substrate is operable to bind the protein produced by the cell; flowing a fluid volume comprising the biomolecule through the chamber via said aperture, wherein the fluid volume is in fluid communication with the capture substrate; and determining a binding interaction between the protein produced by the cell and the biomolecule.

Abstract: Methods and systems are provided for auto-optimization of gamification mechanics. A gamification platform, hosted on any suitable interface, may collect action data from one or more employee. Gamification mechanics are applied to the actions to compute an effectiveness value for each action. An optimization layer of the system may receive performance metrics for the employee from internal systems, or via third party systems. The performance metrics may be employed to update the gamification mechanics by optimizing weights for each action in a fitness function. Effectiveness predictions for future actions are generated using these optimized weights. The updated mechanics and predictions may be provided back to the gamification platform to iteratively repeat the process.

Abstract: Microfluidic devices are provided for trapping, isolating, and processing single cells. The microfluidic devices include a cell capture chamber having a cell funnel positioned within the cell capture chamber to direct a cell passing through the cell capture chamber towards one or more a cell traps positioned downstream of the funnel to receive a cell flowing. The devices may further include auxiliary chambers integrated with the cell capture chamber for subsequent processing and assaying of the contents of a captured cell. Methods for cell capture and preparation are also provided that include flowing cells through a chamber, funneling the cells towards a cell trap, capturing a predefined number of the cells within the trap, interrupting the flow of cells, flowing a wash solution through the chamber to remove contaminants from the chamber, and sealing the predefined number of cells in the chamber.

Abstract: Systems and methods may provide automated compliance and operations management services. User tracking information may be received and stored. The tracking information may be analyzed to generate information for compliance or operations management, which may be provided to a recipient. Systems and methods may automate the process of monitoring, collecting, storing, analyzing, and utilizing data and information. Alerts may be generated. Reports may be compiled and delivered to a reporting entity, and may be used for compliance, tracking, monitoring or operations management. Some data may be fed directly into other existing systems and databases including, but not limited to, payroll processing systems, tax enterprise resource planning systems, human resource (HR) systems, time-keeping systems and billing systems. Proposed systems and methods may be used by individuals, enterprises, or other entities that gather data for regulatory or other compliance or operations management.

Abstract: There are provided methods, and devices for assaying for a binding interaction between a protein, such as a monoclonal antibody, produced by a cell, and a biomolecule. The method may include retaining the cell within a chamber having an aperture; exposing the protein produced by the cell to a capture substrate, wherein the capture substrate is in fluid communication with the protein produced by the cell and wherein the capture substrate is operable to bind the protein produced by the cell; flowing a fluid volume comprising the biomolecule through the chamber via said aperture, wherein the fluid volume is in fluid communication with the capture substrate; and determining a binding interaction between the protein produced by the cell and the biomolecule.

Abstract: The A method and system for server synchronization with a computing device via a companion device is disclosed. In one embodiment, the method comprises synchronizing a device and an enterprise server via a companion device, which comprises retrieving a record extraction sequence from the server; providing the record extraction sequence to the handheld device from the companion device; and extracting records stored on a database according to the record extraction sequence, wherein the extracted records are not already stored on the computing device.

Abstract: A method and system for direct server synchronization with a computing device is disclosed. In one embodiment, the method comprises synchronizing directly a handheld device and an enterprise server, which comprises retrieving a record extraction sequence from the server; and extracting records stored on a database according to the record extraction sequence, wherein the extracted records are not already stored on the computing device.

Abstract: The A method and system for server synchronization with a computing device via a companion device is disclosed. In one embodiment, the method comprises synchronizing a device and an enterprise server via a companion device, which comprises retrieving a record extraction sequence from the server; providing the record extraction sequence to the handheld device from the companion device; and extracting records stored on a database according to the record extraction sequence, wherein the extracted records are not already stored on the computing device.

Abstract: The A method and system for server synchronization with a computing device via a companion device is disclosed. In one embodiment, the method comprises synchronizing a device and an enterprise server via a companion device, which comprises retrieving a record extraction sequence from the server; providing the record extraction sequence to the handheld device from the companion device; and extracting records stored on a database according to the record extraction sequence, wherein the extracted records are not already stored on the computing device.

Abstract: A method and system for direct server synchronization with a computing device is disclosed. In one embodiment, the method comprises synchronizing directly a handheld device and an enterprise server, which comprises retrieving a record extraction sequence from the server; and extracting records stored on a database according to the record extraction sequence, wherein the extracted records are not already stored on the computing device.