Patents by Inventor Anuradha Sekher
Anuradha Sekher has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11268116Abstract: Disclosed are methods and kits for endonuclease-assisted DNA amplification reaction using decontaminated primer solutions that are pre-treated with a nuclease. Nucleic acid amplification assays that employ nuclease-resistant, inosine-containing primers, endonuclease V enzymes to introduce a nick into a target DNA comprising at least one inosine, and a DNA polymerase to generate amplicons of a target DNA are also disclosed.Type: GrantFiled: March 30, 2018Date of Patent: March 8, 2022Assignee: GLOBAL LIFE SCIENCES SOLUTIONS OPERATIONS UK LTDInventors: Robert Scott Duthie, John Richard Nelson, Anuradha Sekher
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Patent number: 10704042Abstract: Methods of amplification, purification and detection of nucleic acid sequences especially RNA are described. One aspect of the method involves the hybridisation and subsequent ligation of a nucleic acid structure to the nucleic acid sequence desired to be manipulated. The methods require that the nucleic acid structure comprises a double stranded region and a single stranded region. The single stranded region is complementary to the RNA sequence of interest. The double stranded region may also contain additional functionalities which are then used subsequently in the method.Type: GrantFiled: April 30, 2018Date of Patent: July 7, 2020Assignee: GE HEALTHCARE BIO-SCIENCES CORP.Inventors: John R. Nelson, R. Scott Duthie, Rohini Dhulipala, Gregory A. Grossman, Anuradha Sekher
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Publication number: 20190062795Abstract: Disclosed are methods and kits for endonuclease-assisted DNA amplification reaction using decontaminated primer solutions that are pre-treated with a nuclease. Nucleic acid amplification assays that employ nuclease-resistant, inosine-containing primers, endonuclease V enzymes to introduce a nick into a target DNA comprising at least one inosine, and a DNA polymerase to generate amplicons of a target DNA are also disclosed.Type: ApplicationFiled: March 30, 2018Publication date: February 28, 2019Inventors: Robert Scott Duthie, John Richard Nelson, Anuradha Sekher
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Publication number: 20180320172Abstract: Methods of amplification, purification and detection of nucleic acid sequences especially RNA are described. One aspect of the method involves the hybridisation and subsequent ligation of a nucleic acid structure to the nucleic acid sequence desired to be manipulated. The methods require that the nucleic acid structure comprises a double stranded region and a single stranded region. The single stranded region is complementary to the RNA sequence of interest. The double stranded region may also contain additional functionalities which are then used subsequently in the method.Type: ApplicationFiled: April 30, 2018Publication date: November 8, 2018Inventors: John R. Nelson, R. Scott Duthie, Rohini Dhulipala, Gregory A. Grossman, Anuradha Sekher
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Patent number: 10100292Abstract: Provided herein are mutant endonuclease V enzymes that are capable of nicking an inosine-containing DNA sequence. Nucleic acid assays and agents that employ such mutant endonuclease V enzymes to introduce a nick into a target DNA including one or more inosine, and uses a DNA polymerase to generate amplicons of a target DNA are also described.Type: GrantFiled: February 19, 2016Date of Patent: October 16, 2018Assignee: General Electric CompanyInventors: John Richard Nelson, Robert Scott Duthie, Gregory Andrew Grossman, Anuradha Sekher
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Patent number: 9951379Abstract: Provided herein are nucleic acid synthesis methods and agents that employ an endonuclease for example, endonuclease V, to introduce a nick into a target DNA including one or more inosine, and uses a DNA polymerase to generate amplicons of the target DNA.Type: GrantFiled: December 20, 2011Date of Patent: April 24, 2018Assignee: GENERAL ELECTRIC COMPANYInventors: John Richard Nelson, Robert Scott Duthie, Carl Williams Fuller, Gregory Andrew Grossmann, Anuradha Sekher
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Publication number: 20160160198Abstract: Provided herein are mutant endonuclease V enzymes that are capable of nicking an inosine-containing DNA sequence. Nucleic acid assays and agents that employ such mutant endonuclease V enzymes to introduce a nick into a target DNA including one or more inosine, and uses a DNA polymerase to generate amplicons of a target DNA are also described.Type: ApplicationFiled: February 19, 2016Publication date: June 9, 2016Inventors: John Richard Nelson, Robert Scott Duthie, Gregory Andrew Grossman, Anuradha Sekher
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Patent number: 9279150Abstract: Provided herein are mutant endonuclease V enzymes that are capable of nicking an inosine-containing DNA sequence. Nucleic acid assays and agents that employ such mutant endonuclease V enzymes to introduce a nick into a target DNA including one or more inosine, and uses a DNA polymerase to generate amplicons of a target DNA are also described.Type: GrantFiled: March 15, 2013Date of Patent: March 8, 2016Assignee: GENERAL ELECTRIC COMPANYInventors: John Richard Nelson, Robert Scott Duthie, Gregory Andrew Grossman, Anuradha Sekher
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Publication number: 20130323795Abstract: Disclosed are methods and kits for endonuclease-assisted DNA amplification reaction using decontaminated primer solutions that are pre-treated with a nuclease. Nucleic acid amplification assays that employ nuclease-resistant, inosine-containing primers, endonuclease V enzymes to introduce a nick into a target DNA comprising at least one inosine, and a DNA polymerase to generate amplicons of a target DNA are also disclosed.Type: ApplicationFiled: August 13, 2013Publication date: December 5, 2013Applicant: General Electric CompanyInventors: Robert Scott Duthie, John Richard Nelson, Anuradha Sekher
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Publication number: 20120196330Abstract: Provided herein are nucleic acid synthesis methods and agents that employ an endonuclease for example, endonuclease V, to introduce a nick into a target DNA including one or more inosine, and uses a DNA polymerase to generate amplicons of the target DNA.Type: ApplicationFiled: December 20, 2011Publication date: August 2, 2012Applicant: GENERAL ELECTRIC COMPANYInventors: John Richard Nelson, Robert Scott Duthie, Carl Williams Fuller, Gregory Andrew Grossmann, Anuradha Sekher
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Patent number: 8202972Abstract: Provided herein are nucleic acid synthesis methods and agents that employ an endonuclease for example, endonuclease V, to introduce a nick into a target DNA including one or more inosine, and uses a DNA polymerase to generate amplicons of the target DNA.Type: GrantFiled: January 10, 2007Date of Patent: June 19, 2012Assignee: General Electric CompanyInventors: John Richard Nelson, Robert Scott Duthie, Carl Williams Fuller, Gregory Andrew Grossmann, Anuradha Sekher
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Patent number: 7892797Abstract: The present invention provides methods, formulation and kits for the synthesis of long nucleic acid fragments. An improved PCR method is provided for amplifying long DNA fragments. In particular, a single thermostable DNA polymerase is used for the rapid amplification of over 10 kb long DNA fragments. Also provided is a method for extending long complementary DNA strands using this single enzyme formulation.Type: GrantFiled: May 5, 2008Date of Patent: February 22, 2011Assignee: GE Healthcare Bio-Sciences Corp.Inventors: Paul Mitsis, Anuradha Sekher, Gyanendra Kumar
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Publication number: 20090170167Abstract: The present invention provides methods, formulation and kits for the synthesis of long nucleic acid fragments. An improved PCR method is provided for amplifying long DNA fragments. In particular, a single thermostable DNA polymerase is used for the rapid amplification of over 10 kb long DNA fragments. Also provided is a method for extending long complementary DNA strands using this single enzyme formulation.Type: ApplicationFiled: May 5, 2008Publication date: July 2, 2009Applicant: GE HEALTHCARE BIO-SCIENCES CORP.Inventors: PAUL MITSIS, ANURADHA SEKHER, GYANENDRA KUMAR
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Publication number: 20090011472Abstract: Provided herein are nucleic acid synthesis methods and agents that employ an endonuclease for example, endonuclease V, to introduce a nick into a target DNA including one or more inosine, and uses a DNA polymerase to generate amplicons of the target DNA.Type: ApplicationFiled: January 10, 2007Publication date: January 8, 2009Applicant: GENERAL ELECTRIC COMPANYInventors: JOHN RICHARD NELSON, ROBERT SCOTT DUTHIE, CARL WILLIAMS FULLER, GREGORY ANDREW GROSSMANN, ANURADHA SEKHER
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Publication number: 20080003602Abstract: Methods of amplification, purification and detection of nucleic acid sequences especially RNA are described. One aspect of the method involves the hybridisation and subsequent ligation of a nucleic acid structure to the nucleic acid sequence desired to be manipulated. The methods require that the nucleic acid structure comprises a double stranded region and a single stranded region. The single stranded region is complementary to the RNA sequence of interest. The double stranded region may also contain additional functionalities which are then used subsequently in the method.Type: ApplicationFiled: December 22, 2005Publication date: January 3, 2008Applicant: GE HEALTHCARE BIO-SCIENCES CORP.Inventors: John Nelson, R. Duthie, Rohini Dhulipala, Gregory Grossmann, Anuradha Sekher
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Patent number: 7125671Abstract: The present invention relates generally to the use of terminal-phosphate-labeled nucleotides having three or more phosphates as substrates for nucleic acid polymerases and their use in DNA amplification. The labels employed are chemiluminescent, fluorescent, electrochemical and chromogenic moieties as well as mass tags and include those that are directly detectable, detectable after enzyme activation or feed into other processes to generate a different signal. The signal generated from the attached dyes may also be used to quantify the amount of amplification. Further provided are stabilizers that enhance the stability of terminal-phosphate labeled nucleoside polyphosphates in aqueous solutions and are useful for reducing non-enzymatic hydrolysis of these nucleotides, hence decrease background.Type: GrantFiled: August 29, 2003Date of Patent: October 24, 2006Assignee: GE Healthcare Bio-Sciences Corp.Inventors: Anup Sood, Shiv Kumar, John Nelson, Carl Fuller, Anuradha Sekher
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Publication number: 20040152104Abstract: The present invention relates generally to the use of terminal-phosphate-labeled nucleotides having three or more phosphates as substrates for nucleic acid polymerases and their use in DNA amplification. The labels employed are chemiluminescent, fluorescent, electrochemical and chromogenic moieties as well as mass tags and include those that are directly detectable, detectable after enzyme activation or feed into other processes to generate a different signal. The signal generated from the attached dyes may also be used to quantify the amount of amplification. Further provided are stabilizers that enhance the stability of terminal-phosphate labeled nucleoside polyphosphates in aqueous solutions and are useful for reducing non-enzymatic hydrolysis of these nucleotides, hence decrease background.Type: ApplicationFiled: August 29, 2003Publication date: August 5, 2004Inventors: Anup Sood, Shiv Kumar, John Nelson, Carl Fuller, Anuradha Sekher