Abstract: Provided is a multi-omics library construction method, a detection method and a related kit. The multi-omics library construction method includes: placing a sectioned sample on a chip having a PolyA capture function for protein capture and mRNA capture, and performing protein capture using a nucleic acid-conjugated antibody; performing reverse transcription on captured mRNA and complementary strand synthesis on a nucleic acid sequence of the nucleic acid-conjugated antibody to respectively obtain cDNA and a complementary strand of the nucleic acid sequence; and performing library construction on the cDNA and the complementary strand of the nucleic acid sequence, thereby obtaining a transcriptome library and a proteome library of the sectioned sample.

Abstract: The present invention provides a sequencing method based on a single fluorescent dye, in which a self-luminescence signal is used to distinguish the sequential incorporation of different nucleotides, thereby realizing the determination of the polynucleotide sequence.

Abstract: A superamphiphobic coating with durable wear resistance and corrosion resistance for an aluminum alloy surface and a preparation method therefor are provided. A superamphiphobic paint includes, in parts by weight: 10-15 parts of modified SiO2 particles, 10-15 parts of modified TiO2 particles, 5-10 parts of PTFE, and epoxy resin matrix. The preparation method for the superamphiphobic coating includes: pre-mixing raw materials, uniformly depositing components in a pre-mixed solution on the aluminum alloy surface by electrophoresis, followed by baking to obtain the superamphiphobic coating. A superamphiphobic paint is pollution-free during production and use, and the superamphiphobic coating has high cross-linking density, low curing shrinkage rate, high wear resistance, high hardness, superhydrophobicity, and oleophobiccity.

Abstract: The disclosure relates to a method for automatically controlling a biochip and an automatic control system, wherein the method comprises the operations of carrying a suction member through a pipette mechanism to suck a reagent, and carrying the suction member that has sucked the reagent through the pipette mechanism to add the reagent to the biochip, and also provides an automatic control system of a biochip using the method for automatically controlling a biochip. The method and system of a biochip may automatically schedule and control a plurality of biochips, so as to complete the labeling and capturing of biological information in tissues, and perform overall collection of the captured products, which meet the requirements of high utilization rate and high degree of automation for a biochip.

Abstract: Provided are a rolling circle amplification method, a method for preparing a sequencing library, and a DNA nanoball prepared therefrom. The rolling circle amplification method includes: sequentially denaturing and annealing a double-stranded DNA and a mediating sequence in a same system, to complementarily pair the mediating sequence with two ends of a denatured single-stranded DNA; simultaneously introducing a ligase and a polymerase into the system to connect the two ends of the single-stranded DNA under action of the ligase; and performing a rolling circle amplification reaction under action of the polymerase by using the mediating sequence as a primer and the single-stranded DNA as a template, to obtain DNA nanoball.

Abstract: Provided is the use of heteroaromatic ring compounds in nucleic acid detection. Particularly, provided are the use of heteroaromatic ring compounds as a nucleic acid protective agent in nucleic acid detection, a method for inhibiting degradation of nucleic acids during nucleic acid detection, a method for detecting a target nucleic acid molecule, and a method for determining a target single-stranded polynucleotide sequence. The present invention further relates to a reagent and a kit containing the heteroaromatic ring compounds.

Abstract: The present invention provides a sequencing method based on a single fluorescent dye, in which a self-luminescence signal is used to distinguish the sequential incorporation of different nucleotides, thereby realizing the determination of the polynucleotide sequence.

Abstract: Provided are a method for detecting spatial information of nucleic acids in a sample, as well as a nucleic acid array used in the method and a method for producing the nucleic acid array.

Abstract: Provided are a rolling circle amplification method, a method for preparing a sequencing library, and a DNA nanoball prepared therefrom. The rolling circle amplification method includes: sequentially denaturing and annealing a double-stranded DNA and a mediating sequence in a same system, to complementarily pair the mediating sequence with two ends of a denatured single-stranded DNA; simultaneously introducing a ligase and a polymerase into the system to connect the two ends of the single-stranded DNA under action of the ligase; and performing a rolling circle amplification reaction under action of the polymerase by using the mediating sequence as a primer and the single-stranded DNA as a template, to obtain DNA nanoball.

Abstract: Provided is a method for sequencing a long-fragment nucleic acid. The nucleic acid molecules each containing a long insert, a first sequencing adapter, and a second sequencing adapter, is used to construct a sequencing library, and the sequencing is performed in segments to sequence the nucleic acids having the long inserts.

Abstract: The present invention provides a method for sequencing polynucleotides. Sequential incorporation of different nucleotides is detected by using the same light emitting signal, thereby achieving the determination of polynucleotide sequences.

Abstract: Provided are a dual-luciferase reporter gene detection system and use thereof. The detection system includes Gaussia luciferase, Pleuromamma xiphias luciferase, and a substrate. The substrate is coelenterazine or a derivative thereof. Compared with other dual-luciferase reporter gene detection systems, the present dual-luciferase reporter gene detection system produces stronger signals and requires simple reaction conditions.

Abstract: Provided are a method for performing position labeling of nucleic acid molecules, a method for constructing a nucleic acid molecule library for transcriptome sequencing, and a kit for implementing the method.

Abstract: A method for sequencing polynucleotides by using nanopores. The present invention relates to, in particular, a method for sequencing polynucleotides by means of covalently binding a polynucleotide binding protein and a nanopore.

Abstract: An intumescent fire-retardant and anti-corrosive coating modified by polyaniline-silicon carbide and a preparation method thereof are provided, the coating includes a compound A and a compound B. The compound A includes a waterborne epoxy resin emulsion, polyaniline-silicon carbide composite material, fire-retardant filler, titanium dioxide, aluminum hydroxide, talc powder, a wetting dispersant, a defoaming agent, a flow agent and water, the compound B includes an epoxy hardener. The polyaniline-silicon carbide composite material is prepared from silicon carbide, phenylamine, protonic acid and an oxidizing agent through an ice bath. The polyaniline-silicon carbide composite material can enhance dispersion in the emulsion through the organic-inorganic composite, and silicon carbide has high thermal stability and good density, while polyaniline serves as a material with unique fire-retardant properties.

Abstract: The present application relates to transcriptome sequencing and biomolecule space information detection. Specifically, the present application relates to a method for positioning and labeling a nucleic acid molecule, a method for constructing a nucleic acid molecule library for transcriptome sequencing, and a kit for implementing the method.

Abstract: Provided are a method for positioning and labeling a nucleic acid molecule, and a method for constructing a nucleic acid molecule library for single-cell transcriptome sequencing, which relate to the technical fields of single-cell transcriptome sequencing and biomolecular space information detection. Further provided are a nucleic acid molecule library constructed by using the method, and a kit for implementing the method.

Abstract: A DNA library sequence for increasing the number of chip probes and an application thereof. The DNA library sequence comprises two or more position information labeling sequences and three or more fixed sequences, wherein the position information labeling sequences and the fixed sequences are arranged at intervals. The DNA library sequence increases the number of probes of a chip used for spatiotemporal omics, and then a capture number of transcript genes is increased. The present invention has a very high application prospect.

Abstract: Provided are a detection structure and method, a detection chip, and a sensing device. The detection structure (200) includes a sensing device (100) at least including a detection chip (000), a fluid tank (101), and a carrier plate (102), and a detector (201) configured to capture and analyze a signal generated in the sensing device (100). The fluid tank (101) is disposed at the carrier plate (102) and forms a cavity (103) with the carrier plate (102). The detection chip (000) is located in the cavity (103) and at least includes a substrate (001), a first electrode (002) and a first circuit (003) that are disposed at the substrate. The first electrode (002) is connected to a second electrode (004) through the first circuit (003) to form an electrical circuit.

Abstract: Provided is a method for obtaining a double-stranded sequence by single-stranded rolling circle amplification, comprising: 1) performing rolling circle amplification reaction on single-stranded circular DNA by means of a first primer to obtain an amplified sequence, the first primer being complementary to a partial region of the single-stranded circular DNA, and the single-stranded circular DNA having a break mechanism that can cause the single-stranded circular DNA to ring-open; 2) ring-opening the single-stranded circular DNA by means of the break mechanism to obtain single-stranded linear DNA; and 3) using the single-stranded linear DNA as a second primer and using the amplified sequence obtained in step 1) as a template to perform amplification reaction to obtain an amplified double-stranded sequence.