Patents by Inventor Aren Ewing
Aren Ewing has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20230112730Abstract: Provided are methods and compositions for preparing a library of target nucleic acid sequences that are useful for assessing gene mutations for oncology biomarker profiling of samples. In particular, a target-specific primer panel is provided that allows for selective amplification of oncology biomarker target sequences in a sample. In one aspect, the invention relates to target-specific primers useful for selective amplification of one or more target sequences associated with oncology biomarkers from two or more sample types. In some aspects, amplified target sequences obtained using the disclosed methods, and compositions can be used in various processes including nucleic acid sequencing and used to detect the presence of genetic variants of one or more targeted sequences associated with oncology.Type: ApplicationFiled: August 15, 2022Publication date: April 13, 2023Applicant: Life Technologies CorporationInventors: David Chi, Aren Ewing, Na Li, Zunping Luo, Amir Marcovitz
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Patent number: 11447832Abstract: Provided are methods and compositions for preparing a library of target nucleic acid sequences that are useful for assessing gene mutations for oncology biomarker profiling of samples. In particular, a target-specific primer panel is provided that allows for selective amplification of oncology biomarker target sequences in a sample. In one aspect, the invention relates to target-specific primers useful for selective amplification of one or more target sequences associated with oncology biomarkers from two or more sample types. In some aspects, amplified target sequences obtained using the disclosed methods, and compositions can be used in various processes including nucleic acid sequencing and used to detect the presence of genetic variants of one or more targeted sequences associated with oncology.Type: GrantFiled: August 28, 2020Date of Patent: September 20, 2022Assignee: Life Technologies CorporationInventors: David Chi, Aren Ewing, Na Li, Zunping Luo, Amir Marcovitz
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Publication number: 20220251669Abstract: The present disclosure provides compositions and methods, as well as combinations, kits, and systems that include the compositions and methods, for amplification, detection, characterization, assessment, profiling and/or measurement of nucleic acids in samples, particularly biological samples. Compositions and methods provided herein include combinations of microbial species target-specific nucleic acid primers for selective amplification and/or combinations of primers for amplification of nucleic acids from a large group of taxonomically related microorganisms. In one aspect, amplified nucleic acids obtained using the compositions and methods can be used in various processes including nucleic acid sequencing and used to detect the presence of microbial species and assess microbial populations in a variety of samples.Type: ApplicationFiled: April 8, 2022Publication date: August 11, 2022Inventors: Shrutii SARDA, Anna MCGEACHY, Rajesh GOTTIMUKKALA, David MERRILL, Heesun SHIN, Aren EWING, Wing LEE, Birgit DREWS, Fiona HYLAND, Janice AU-YOUNG
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Publication number: 20210062271Abstract: Provided are methods and compositions for preparing a library of target nucleic acid sequences that are useful for assessing gene mutations for oncology biomarker profiling of samples. In particular, a target-specific primer panel is provided that allows for selective amplification of oncology biomarker target sequences in a sample. In one aspect, the invention relates to target-specific primers useful for selective amplification of one or more target sequences associated with oncology biomarkers from two or more sample types. In some aspects, amplified target sequences obtained using the disclosed methods, and compositions can be used in various processes including nucleic acid sequencing and used to detect the presence of genetic variants of one or more targeted sequences associated with oncology.Type: ApplicationFiled: August 28, 2020Publication date: March 4, 2021Inventors: David CHI, Aren EWING, Na LI, Zunping LUO, Amir MARCOVITZ
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Publication number: 20200392470Abstract: Disclosed are microalgal cells having an ablated or downregulated fatty acyl-ACP thioesterase (FATA) gene, wherein the cell is modified to express a heterologous lysophosphatidic acid acyltransferase (LPAAT) comprising an amino acid sequence that has at least 80% identity to an acyltransferase encoded by SEQ ID NO: 90, 89, 92, 93 or 95 and wherein the modified microalgal cell produces an oil with an elevated ratio of saturated-unsaturated-saturated triglycerides over trisaturated triglycerides as compared to a corresponding unmodified cell. Also disclosed are microalgal oils comprising at least 60% stearate-oleate-stearate (SOS) triglycerides, less than 5% trisaturates and wherein the fatty acid profile of the oil comprises at least 50% C18:0. Related methods of producing an oil are also disclosed.Type: ApplicationFiled: August 20, 2020Publication date: December 17, 2020Inventors: Jeffrey Leo Moseley, Jason Casolari, Xinhua Zhao, Aren Ewing, Aravind Somanchi, Scott Franklin, David Davis
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Publication number: 20190345463Abstract: The present invention relates to beta-ketoacyl ACP synthase genes of the KASI/KASIV type and proteins encoded by these genes. The genes can be included in nucleic acid constructs, vectors or host cells. Expression of the gene products can alter the fatty acid profile of host cells. The KAS genes can be combined with a FATA or FATB thioesterase gene to create a cell that produces an increased amount of C8-C16 fatty acids. Suitable host cells include plastidic cells of plants or microalgae. Oleaginous microalga host cells with the new genes are disclosed.Type: ApplicationFiled: April 18, 2019Publication date: November 14, 2019Inventors: David Davis, George Rudenko, Aravind Somanchi, Jason Casolari, Scott Franklin, Aren Ewing
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Patent number: 10316299Abstract: The present invention relates to beta-ketoacyl ACP synthase genes of the KASI/KASIV type and proteins encoded by these genes. The genes can be included in nucleic acid constructs, vectors or host cells. Expression of the gene products can alter the fatty acid profile of host cells. The KAS genes can be combined with a FATA or FATB thioesterase gene to create a cell that produces an increased amount of C8-C16 fatty acids. Suitable host cells include plastidic cells of plants or microalgae. Oleaginous microalga host cells with the new genes are disclosed.Type: GrantFiled: April 10, 2018Date of Patent: June 11, 2019Assignee: CORBION BIOTECH, INC.Inventors: David Davis, George N. Rudenko, Aravind Somanchi, Jason Casolari, Scott Franklin, Aren Ewing
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Publication number: 20180230442Abstract: The present invention relates to beta-ketoacyl ACP synthase genes of the KASI/KASIV type and proteins encoded by these genes. The genes can be included in nucleic acid constructs, vectors or host cells. Expression of the gene products can alter the fatty acid profile of host cells. The KAS genes can be combined with a FATA or FATB thioesterase gene to create a cell that produces an increased amount of C8-C16 fatty acids. Suitable host cells include plastidic cells of plants or microalgae. Oleaginous microalga host cells with the new genes are disclosed.Type: ApplicationFiled: April 10, 2018Publication date: August 16, 2018Inventors: David Davis, George N. Rudenko, Aravind Somanchi, Jason Casolari, Scott Franklin, Aren Ewing
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Publication number: 20180142218Abstract: Recombinant nucleic acids and vector constructs encoding acyltransferases and variant thioesterases, and the acyltransferases and variant thioesterases encoded by the nucleic acids are provided. The acyltransferases and variant thioesterases are useful in fatty acid synthesis and triacylglycerol production. Host cells that express the recombinant nucleic acids as well as methods of cultivating the host cells, methods of producing oils from the host cells are provided. The recombinant host cells and the oils produced therefrom have altered fatty acid profiles and/or triacylglycerols with altered regiospecificity.Type: ApplicationFiled: October 4, 2017Publication date: May 24, 2018Inventors: Jeffrey Leo Moseley, Jason Casolari, Xinhua Zhao, Aren Ewing, Aravind Somanchi, Scott Franklin, David Davis
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Patent number: 9969990Abstract: The present invention relates to beta-ketoacyl ACP synthase genes of the KASI/KASIV type and proteins encoded by these genes. The genes can be included in nucleic acid constructs, vectors or host cells. Expression of the gene products can alter the fatty acid profile of host cells. The KAS genes can be combined with a FATA or FATB thioesterase gene to create a cell that produces an increased amount of C8-C16 fatty acids. Suitable host cells include plastidic cells of plants or microalgae. Oleaginous microalga host cells with the new genes are disclosed.Type: GrantFiled: July 10, 2015Date of Patent: May 15, 2018Assignee: Corbion Biotech, Inc.Inventors: David Davis, George Rudenko, Aravind Somanchi, Jason Casolari, Scott Franklin, Aren Ewing
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Patent number: 9518277Abstract: Genetically engineered microbial, e.g., Chlorella protothecoides, cells producing microbial oils are useful as a food additive and a source of renewable fuels and industrial chemicals. Lipid biosynthesis genes for Auxenochlorella protethecoides are disclosed that are useful for increasing lipid production and altering fatty acid and triacylglycerol profiles in recombinant microrganisms including cells of the genus Auxenochlorella or Prototheca.Type: GrantFiled: December 6, 2013Date of Patent: December 13, 2016Assignee: TerraVia Holdings, Inc.Inventors: Scott Franklin, Shane Brubaker, George N. Rudenko, Riyaz Bhat, Matthew Shoa-Azar, Aravind Somanchi, Aren Ewing
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Publication number: 20160010066Abstract: The present invention relates to beta-ketoacyl ACP synthase genes of the KASI/KASIV type and proteins encoded by these genes. The genes can be included in nucleic acid constructs, vectors or host cells. Expression of the gene products can alter the fatty acid profile of host cells. The KAS genes can be combined with a FATA or FATB thioesterase gene to create a cell that produces an increased amount of C8-C16 fatty acids. Suitable host cells include plastidic cells of plants or microalgae. Oleaginous microalga host cells with the new genes are disclosed.Type: ApplicationFiled: July 10, 2015Publication date: January 14, 2016Inventors: David Davis, George Rudenko, Aravind Somanchi, Jason Casolari, Scott Franklin, Aren Ewing
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Publication number: 20140178950Abstract: Genetically engineered microbial, e.g., Chlorella protothecoides, cells producing microbial oils are useful as a food additive and a source of renewable fuels and industrial chemicals. Lipid biosynthesis genes for Auxenochlorella protethecoides are disclosed that are useful for increasing lipid production and altering fatty acid and triacylglycerol profiles in recombinant microrganisms including cells of the genus Auxenochlorella or Prototheca.Type: ApplicationFiled: December 6, 2013Publication date: June 26, 2014Applicant: Solazyme, Inc.Inventors: Scott Franklin, Shane Brubaker, George N. Rudenko, Riyaz Bhat, Matthew Shoa-Azar, Aravind Somanchi, Aren Ewing