Patents by Inventor Ariffeen Bongso
Ariffeen Bongso has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 10413574Abstract: The invention is directed to a method of treating a wound (e.g., to suppress scar formation) in an individual in need thereof comprising contacting the wound with an effective amount of a composition comprising (i) Wharton's jelly stem cells (WJSCs), (ii) a cell culture medium that has been conditioned with WJSCs, (iii) a lysate of WJSCs, (iv) a cell culture medium that has been conditioned with WJSCs exposed to apoptotic skin cells and keloid cells, or (v) a combination thereof. The invention is directed to a medical dressing (e.g., pharmaceutical compositions) comprising Wharton's jelly stem cells (WJSCs), a cell culture medium that has been conditioned with WJSCs, a lysate of WJSCs, a cell culture medium that has been conditioned with WJSCs exposed to apoptotic skin cells and keloid cells, or a combination thereof.Type: GrantFiled: August 15, 2013Date of Patent: September 17, 2019Assignee: National University of SingaporeInventors: Chui Yee Fong, Mahesh Choolani, Arijit Biswas, Tuan Ariffeen Bongso, Seeram Ramakrishna
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Patent number: 9910032Abstract: There is provided a method of identifying at least one foetal erythroblast in a sample, the method comprising analyzing the morphology of at least one cell in the sample; wherein at least one analyzed cell that is nucleated, is CD45 negative and comprises a relatively high cytoplasmic to nuclear ratio is identified as the foetal erythroblast.Type: GrantFiled: April 11, 2012Date of Patent: March 6, 2018Assignee: National University of SingaporeInventors: Chui Yee Fong, Ariffeen Bongso, Mahesh Choolani, Zhouwei Huang, Gauthaman Kalamegam, Sukumar Ponnusamy
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Patent number: 9402388Abstract: The invention is directed to a method of freezing stem cells comprising introducing the HSCs into a cell culture medium that has been conditioned with Wharton's Jelly mesenchymal stem cells (WJSCs), thereby producing a stem cell culture, and slowly freezing the stem cell culture. In other aspects, the invention is directed to compositions comprising stem cells produced by the methods provided herein. In yet other aspects, the invention is directed to pharmaceutical compositions comprising the stem cells produced by the methods provided herein.Type: GrantFiled: November 1, 2013Date of Patent: August 2, 2016Assignee: National University of SingaporeInventors: Chui Yee Fong, Tuan Ariffeen Bongso, Daniel Hao Lin
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Patent number: 9315776Abstract: In one aspect, the invention is directed to methods of expanding hematopoietic stem cells (HSCs) comprising culturing the HSCs with Wharton's Jelly mesenchymal stem cells (WJSCs), a cell culture medium that has been conditioned with WJSCs, or a combination thereof, thereby producing a HSC culture; and maintaining the HSC culture under conditions in which the HSCs expand in the culture, thereby expanding the HSCs. In another aspect, the invention is directed to a method of transplanting the expanded HSCs in an individual in need thereof. In yet another aspect, the invention is directed to compositions comprising HSCs and Wharton's Jelly mesenchymal stem cells (WJSCs). The composition can further comprise a cell culture medium that has been conditioned with WJSCs.Type: GrantFiled: November 2, 2012Date of Patent: April 19, 2016Assignee: National University of SingaporeInventors: Chui Yee Fong, Tuan Ariffeen Bongso, Arijit Biswas
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Publication number: 20150352157Abstract: The invention is directed to a method of treating a wound (e.g., to suppress scar formation) in an individual in need thereof comprising contacting the wound with an effective amount of a composition comprising (i) Wharton's jelly stem cells (WJSCs). (ii) a cell culture medium that has been conditioned with WJSCs. (iii) a lysate of WJSCs. (iv) a cell culture medium that has been conditioned with WJSCs exposed to apoptotic skin cells and keloid cells, or (v) a combination thereof. The invention is directed to a medical dressing (e.g., pharmaceutical compositions) comprising Wharton's jelly stem cells (WJSCs), a cell culture medium that has been conditioned with WJSCs, a lysate of WJSCs, a cell culture medium that has been conditioned with WJSCs exposed to apoptotic skin cells and keloid cells, or a combination thereof.Type: ApplicationFiled: August 15, 2013Publication date: December 10, 2015Inventors: Chui Yee Fong, Mahesh Choolani, Arijit Biswas, Tuan Ariffeen Bongso, Seeram Ramakrishna
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Publication number: 20140120615Abstract: The invention is directed to a method of freezing stem cells comprising introducing the HSCs into a cell culture medium that has been conditioned with Wharton's Jelly mesenchymal stem cells (WJSCs), thereby producing a stem cell culture, and slowly freezing the stem cell culture. In other aspects, the invention is directed to compositions comprising stem cells produced by the methods provided herein. In yet other aspects, the invention is directed to pharmaceutical compositions comprising the stem cells produced by the methods provided herein.Type: ApplicationFiled: November 1, 2013Publication date: May 1, 2014Applicant: NATIONAL UNIVERSITY OF SINGAPOREInventors: Chui Yee Fong, Tuan Ariffeen Bongso, Daniel Hao Lin
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Publication number: 20140030724Abstract: There is provided a method of identifying at least one foetal erythroblast in a sample, the method comprising analysing the morphology of at least one cell in the sample; wherein at least one analysed cell that is nucleated, is CD45 negative and comprises a relatively high cytoplasmic to nuclear ratio is identified as the foetal erythroblast.Type: ApplicationFiled: April 11, 2012Publication date: January 30, 2014Applicant: NATIONAL UNIVERSITY OF SINGAPOREInventors: Chui Yee Fong, Ariffeen Bongso, Mahesh Choolani, Zhouwei Huang, Gauthaman Kalamegam, Sukumar Ponnusamy
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Publication number: 20130302285Abstract: In one aspect, the invention is directed to methods of expanding hematopoietic stem cells (HSCs) comprising culturing the HSCs with Wharton's Jelly mesenchymal stem cells (WJSCs), a cell culture medium that has been conditioned with WJSCs, or a combination thereof, thereby producing a HSC culture; and maintaining the HSC culture under conditions in which the HSCs expand in the culture, thereby expanding the HSCs. In another aspect, the invention is directed to a method of transplanting the expanded HSCs in an individual in need thereof. In yet another aspect, the invention is directed to compositions comprising HSCs and Wharton's Jelly mesenchymal stem cells (WJSCs). The composition can further comprise a cell culture medium that has been conditioned with WJSCs.Type: ApplicationFiled: November 2, 2012Publication date: November 14, 2013Applicant: NATIONAL UNIVERSITY OF SINGAPOREInventors: Chui Yee Fong, Tuan Ariffeen Bongso, Arijit Biswas
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Publication number: 20050164383Abstract: The present invention relates to undifferentiated human embryonic stem cells, methods of cultivation and propagation, production of differentiated cells and in particular the production of human embryonic stem cells capable of yielding somatic differentiated cells in vitro, as well as committed progenitor cells capable of giving rise to mature somatic cells and uses thereof. The present invention also provides a purified preparation of undifferentiated human embryonic stem cells capable of proliferation in vitro. Furthermore, the present invention provides a somatic cell differentiated in vitro from an undifferentiated embryonic stem cell. There is also provided a committed progenitor cell capable of giving rise to mature somatic cells.Type: ApplicationFiled: February 1, 2005Publication date: July 28, 2005Inventors: Benjamin Reubinoff, Martin Pera, Chui-Yee Fong, Alan Trounson, Ariffeen Bongso
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Patent number: 6875607Abstract: The present invention relates to undifferentiated human embryonic stem cells, methods of cultivation and propagation, production of differentiated cells and in particular the production of human embryonic stem cells capable of yielding somatic differentiated cells in vitro, as well as committed progenitor cells capable of giving rise to mature somatic cells and uses thereof. The present invention also provides a purified preparation of undifferentiated human embryonic stem cells capable of proliferation in vitro. Furthermore, the present invention provides a somatic cell differentiated in vitro from an undifferentiated embryonic stem cell. There is also provided a committed progenitor cell capable of giving rise to mature somatic cells.Type: GrantFiled: November 9, 1999Date of Patent: April 5, 2005Assignee: ES Cell International Pte LtdInventors: Benjamin Eithan Reubinoff, Martin Frederick Pera, Chui-Yee Fong, Alan Osborne Trounson, Ariffeen Bongso
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Publication number: 20040253721Abstract: The present invention relates to the field of stem cell culture, in particular undifferentiated stem cell culture and to methods for derivation and propagation of such cells. More particularly, the invention relates to derivation and propagation of undifferentiated HES cells on human feeder layers and/or in the absence of a feeder layer. The human feeder layers may be selected from the group including human fetal muscle (HFM), human fetal skin (HFS), human adult fallopian tube (HAFT) fibroblasts and human adult skin cells. They may be cultured in the presence of a suitable medium selected from the group including Human Embryonic Stem Cell (HES), Knockout (KO), or Human Feeder (HF) medium supplemented with or without human serum.Type: ApplicationFiled: March 29, 2004Publication date: December 16, 2004Inventors: Ariffeen Bongso, Mark Richards, Fong Chui-Yee, Chan Woon-Khiong
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Publication number: 20030143736Abstract: The present invention relates to the field of stem cell culture, in particular undifferentiated stem cell culture and to methods for derivation and propagation of such cells. More particularly, the invention relates to derivation and propagation of undifferentiated HES cells on human feeder layers and/or in the absence of a feeder layer.Type: ApplicationFiled: September 30, 2002Publication date: July 31, 2003Inventors: Ariffeen Bongso, Mark Richards, Fong Chui-Yee, Chan Woon-Khiong
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Publication number: 20020160509Abstract: The present invention relates to undifferentiated human embryonic stem cells, methods of cultivation and propagation, production of differentiated cells and in particular the production of human embryonic stem cells capable of yielding somatic differentiated cells in vitro, as well as committed progenitor cells capable of giving rise to mature somatic cells and uses thereof. The present invention also provides a purified preparation of undifferentiated human embryonic stem cells capable of proliferation in vitro. Furthermore, the present invention provides a somatic cell differentiated in vitro from an undifferentiated embryonic stem cell. There is also provided a committed progenitor cell capable of giving rise to mature somatic cells.Type: ApplicationFiled: March 5, 2002Publication date: October 31, 2002Inventors: Benjamin Eithan Reubinoff, Martin Frederick Pera, Chui-Yee Fong, Alan Osborne Trounson, Ariffeen Bongso