Patents by Inventor Arisa Murakami

Arisa Murakami has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Method for identifying polyubiquitinated substrate
    Patent number: 10634684
    Abstract: An object of the present invention is to provide a method for efficiently identifying a polyubiquitinated substrate which is generally not easily identified. The method for identifying a polyubiquitinated substrate includes (1) a step of expressing a trypsin-resistant polyubiquitin chain-binding protein and a ubiquitin ligase in a cell, (2) a step of isolating a complex that contains the trypsin-resistant polyubiquitin chain-binding protein from the cell having undergone the step (1), (3) a step of subjecting the complex isolated by the step (2) to trypsin digestion, and (4) a step of identifying a peptide that has a ubiquitination site from a digested material obtained by the step (3).
    Type: Grant
    Filed: November 13, 2014
    Date of Patent: April 28, 2020
    Assignee: TOKYO METROPOLITAN INSTITUTE OF MEDICAL SCIENCE
    Inventors: Yukiko Yoshida, Yasushi Saeki, Hikaru Tsuchiya, Arisa Murakami, Keiji Tanaka
  • Method for Identifying Polyubiquitinated Substrate
    Publication number: 20180267052
    Abstract: An object of the present invention is to provide a method for efficiently identifying a polyubiquitinated substrate which is generally not easily identified. The method for identifying a polyubiquitinated substrate includes (1) a step of expressing a trypsin-resistant polyubiquitin chain-binding protein and a ubiquitin ligase in a cell, (2) a step of isolating a complex that contains the trypsin-resistant polyubiquitin chain-binding protein from the cell having undergone the step (1), (3) a step of subjecting the complex isolated by the step (2) to trypsin digestion, and (4) a step of identifying a peptide that has a ubiquitination site from a digested material obtained by the step (3).
    Type: Application
    Filed: November 13, 2014
    Publication date: September 20, 2018
    Inventors: Yukiko Yoshida, Yasushi Saeki, Hikaru Tsuchiya, Arisa Murakami, Keiji Tanaka