Patents by Inventor Arjan G. J. Tibbe

Arjan G. J. Tibbe has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20180208998
    Abstract: The invention relates generally to the field of the identification of DNA sequences, genes or chromosomes. Methods and composition to obtain Unique Sequence DNA probes are provided. Compositions comprised of and double stranded DNA containing Unique Sequences from which the repetitive sequences are eliminated according to the method described in this invention. The invention also relates to the preservation of cells that have been identified after immunomagnetic selection and fluorescent labeling in order to further interrogate the cells of interest. Furthermore the invention relates to genetic analysis of cells that have been identified after immunomagnetic selection and fluorescent labeling.
    Type: Application
    Filed: March 23, 2018
    Publication date: July 26, 2018
    Applicant: Menarini Silicon Biosystems, Inc.
    Inventors: John Verrant, Mark Carle CONNELLY, Brad FOULK, Michael T. KAGAN, Joost F. SWENNENHUIS, Leon W.M.M. TERSTAPPEN, Arjan G.J. TIBBE
  • Patent number: 9957571
    Abstract: The invention relates generally to the field of the identification of DNA sequences, genes or chromosomes. Methods and composition to obtain Unique Sequence DNA probes are provided. Compositions comprises of and double stranded DNA containing Unique Sequences from which the repetitive sequences are eliminated according to the method described in this invention. The invention also relates to the preservation of cells that have been identified after immunomagnetic selection and fluorescent labeling in order to further interrogate the cells of interest. Furthermore the invention relates to genetic analysis of cells that have been identified after immunomagnetic selection and fluorescent labeling.
    Type: Grant
    Filed: June 23, 2015
    Date of Patent: May 1, 2018
    Assignee: Menarini Silicon Biosystems, Inc.
    Inventors: John Verrant, Mark Carle Connelly, Brad Foulk, Michael T Kagan, Joost F Swennenhuis, Leon W. M. M. Terstappen, Arjan G. J. Tibbe
  • Publication number: 20160046998
    Abstract: The invention relates generally to the field of the identification of DNA sequences, genes or chromosomes. Methods and composition to obtain Unique Sequence DNA probes are provided. Compositions comprises of and double stranded DNA containing Unique Sequences from which the repetitive sequences are eliminated according to the method described in this invention. The invention also relates to the preservation of cells that have been identified after immunomagnetic selection and fluorescent labeling in order to further interrogate the cells of interest. Furthermore the invention relates to genetic analysis of cells that have been identified after immunomagnetic selection and fluorescent labeling.
    Type: Application
    Filed: June 23, 2015
    Publication date: February 18, 2016
    Inventors: John Verrant, Mark Carle Connelly, Brad Foulk, Michael T Kagan, Joost F Swennenhuis, Leon W.M.M. Terstappen, Arjan G.J. Tibbe
  • Patent number: 9127302
    Abstract: The invention relates generally to the field of identification of DNA sequences, genes or chromosomes. Methods and composition to obtain Unique Sequence DNA probes are provided. Composition comprises of any double stranded DNA containing Unique Sequences from which the repetitive sequences are eliminated according to the method described in this invention. The invention also relates to the preservation of cells that have been identified after immunomagnetic selection and fluorescent labeling in order to further interrogate the cells of interest. Furthermore the invention relates to genetic analysis of cells that have been identified after immunomagnetic selection and fluorescent labeling.
    Type: Grant
    Filed: September 20, 2006
    Date of Patent: September 8, 2015
    Assignee: Janssen Diagnostics, LLC
    Inventors: John A Verrant, Arjan G. J. Tibbe, Brad Foulk, Joost F. Swennenhuis, Leon W. M. M. Terstappen, Mark Carle Connelly, Michael T. Kagan
  • Patent number: 8569077
    Abstract: A method for removing excess unbound ferrofluid and imaging immunomagnetically enriched circulating tumor cells is provided. A vessels having a preformed grooves in the viewing surface is optimally designed for cell alignment and imaging. After separating the unbound particles by centrifugation, an externally-applied force is applied to transport magnetically responsive particle-CTC complex toward the transparent collection wall. The grooved inner surface of the viewing face of the chamber provide uniform distribution of the particles for easy imaging. The invention is also useful in conducting quantitative analysis and sample preparation in conjunction with automated cell enumeration techniques as in quantitative analysis of CTC in disease.
    Type: Grant
    Filed: May 18, 2009
    Date of Patent: October 29, 2013
    Assignee: Veridex, LLC
    Inventors: Tyco M. Scholtens, Frederik Schreuder, Jan Greve, Arjan G. J. Tibbe, Leon W. M. M. Terstappen
  • Patent number: 8189899
    Abstract: The enumeration of cells in fluids by flow cytometry is widely used across many disciplines such as assessment of leukocyte subsets in different bodily fluids or of bacterial contamination in environmental samples, food products and bodily fluids. For many applications the cost, size and complexity of the instruments prevents wider use, for example, CD4 analysis in HIV monitoring in resource-poor countries. The novel device, methods and algorithms disclosed herein largely overcome these limitations. Briefly, all cells in a biological sample are fluorescently labeled, but only the target cells are also magnetically labeled. In addition, non-magnetically labeled cells are imaged for viability in a modified slide configuration. The labeled sample, in a chamber or cuvet, is placed between two wedge-shaped magnets to selectively move the magnetically labeled cells to the observation surface of the cuvet.
    Type: Grant
    Filed: February 17, 2010
    Date of Patent: May 29, 2012
    Assignee: Veridex, LLC
    Inventors: Frank A. W. Coumans, Jan Greve, Frank P. Modica, Leon W. M. M. Terstappen, Arjan G. J. Tibbe, John A. Verrant
  • Publication number: 20120055854
    Abstract: A filtering system for separating unbound ferrofluid from bound ferrofluid in the enrichment of a target entity in a biological sample. The filtering device of the present invention has application in the isolation target cells from unbound ferrofluid during separation with a permanent magnet (floater) mechanism. This process reduces interference of unbound ferrofluid during subsequent image analysis or enumeration of cells by image cytometry. The system has application in the assessment of target populations such as leukocyte subsets in different bodily fluids or bacterial contamination in environmental samples, food products and bodily fluids. Briefly, fluorescently labeled target cells are linked to magnetic particles or beads. The linkage process results in a mixture having a population of contaminating unbound magnetic particles. In one embodiment for separation, a small, permanent magnet is inserted directly into the chamber containing the labeled cells.
    Type: Application
    Filed: February 5, 2010
    Publication date: March 8, 2012
    Inventor: Arjan G.J. Tibbe
  • Patent number: 8128890
    Abstract: The enumeration of cells in fluids by flow cytometry is widely used across many disciplines such as assessment of leukocyte subsets in different bodily fluids or of bacterial contamination in environmental samples, food products and bodily fluids. For many applications the cost, size and complexity of the instruments prevents wider use, for example, CD4 analysis in HIV monitoring in resource-poor countries. The novel device, methods and algorithms disclosed herein largely overcome these limitations. Briefly, all cells in a biological sample are fluorescently labeled, but only the target cells are also magnetically labeled. The labeled sample, in a chamber or cuvet, is placed between two wedge-shaped magnets to selectively move the magnetically labeled cells to the observation surface of the cuvet. An LED illuminates the cells and a CCD camera captures the images of the fluorescent light emitted by the target cells.
    Type: Grant
    Filed: November 1, 2010
    Date of Patent: March 6, 2012
    Assignee: Veridex, LLC
    Inventors: Erik Droog, Dhanesh Gohel, Arjan G. J. Tibbe, Jan Greve, Leon W. M. M. Terstappen
  • Patent number: 8110101
    Abstract: A system for enumeration of cells in fluids by image cytometry is described for assessment of target populations such as leukocyte subsets in different bodily fluids or bacterial contamination in environmental samples, food products and bodily fluids. Briefly, all cells in a biological sample are fluorescently labeled, but only the target cells are also magnetically labeled. A small, permanent magnet is inserted directly into the chamber containing the labeled sample. The magnets are coated with PDMS silicone rubber to provide a smooth and even surface which allows imaging on a single focal plane. The cells are illuminated and the images of the fluorescent light emitted by the target cells are captured by a CCD camera. Image analysis performed with a novel algorithm provides a count of the cells on the surface that can be related to the target cell concentration of the original sample.
    Type: Grant
    Filed: August 30, 2007
    Date of Patent: February 7, 2012
    Assignee: Veridex, LLC
    Inventors: Arjan G. J. Tibbe, Leon W. M. M. Terstappen
  • Publication number: 20110052037
    Abstract: The enumeration of cells in fluids by flow cytometry is widely used across many disciplines such as assessment of leukocyte subsets in different bodily fluids or of bacterial contamination in environmental samples, food products and bodily fluids. For many applications the cost, size and complexity of the instruments prevents wider use, for example, CD4 analysis in HIV monitoring in resource-poor countries. The novel device, methods and algorithms disclosed herein largely overcome these limitations. Briefly, all cells in a biological sample are fluorescently labeled, but only the target cells are also magnetically labeled. In addition, non-magnetically labeled cells are imaged for viability in a modified slide configuration. The labeled sample, in a chamber or cuvet, is placed between two wedge-shaped magnets to selectively move the magnetically labeled cells to the observation surface of the cuvet.
    Type: Application
    Filed: February 17, 2010
    Publication date: March 3, 2011
    Applicant: VERIDEX, LLC
    Inventors: Frank A.W. Coumans, Jan Greve, Frank P. Modica, Leon W.M.M. Terstappen, Arjan G.J. Tibbe, John A. Verrant
  • Publication number: 20110014686
    Abstract: A system for enumeration of cells in fluids by image cytometry is described for assessment of target populations such as leukocyte subsets in different bodily fluids or bacterial contamination in environmental samples, food products and bodily fluids. Briefly, fluorescently labeled target cells are linked to magnetic particles or beads. In one embodiment, a small, permanent magnet is inserted directly into the chamber containing the labeled cells. The magnets are coated with PDMS silicone rubber to provide a smooth and even surface which allows imaging on a single focal plane. The magnet is removed from the sample and illuminated with fluorescent light emitted by the target cells captured by a CCD camera. In another embodiment, a floater having a permanent magnet allows the target cells to line up along a single imaging plane within the sample solution.
    Type: Application
    Filed: September 23, 2010
    Publication date: January 20, 2011
    Inventors: Arjan G.J. Tibbe, Leon W.M.M. Terstappen
  • Patent number: 7828968
    Abstract: A system for enumeration of cells in fluids by image cytometry is described for assessment of target populations such as leukocyte subsets in different bodily fluids or bacterial contamination in environmental samples, food products and bodily fluids. Briefly, fluorescently labeled target cells are linked to magnetic particles or beads. In one embodiment, a small, permanent magnet is inserted directly into the chamber containing the labeled cells. The magnets are coated with PDMS silicone rubber to provide a smooth and even surface which allows imaging on a single focal plane. The magnet is removed from the sample and illuminated with fluorescent light emitted by the target cells captured by a CCD camera. In another embodiment, a floater having a permanent magnet allows the target cells to line up along a single imaging plane within the sample solution.
    Type: Grant
    Filed: February 15, 2008
    Date of Patent: November 9, 2010
    Assignee: Veridex, LLC
    Inventors: Arjan G. J. Tibbe, Leon W. M. M. Terstappen
  • Patent number: 7764821
    Abstract: The enumeration of cells in fluids by flow cytometry is widely used across many disciplines such as assessment of leukocyte subsets in different bodily fluids or of bacterial contamination in environmental samples, food products and bodily fluids. For many applications the cost, size and complexity of the instruments prevents wider use, for example, CD4 analysis in HIV monitoring in resource-poor countries. The novel device, methods and algorithms disclosed herein largely overcome these limitations. Briefly, all cells in a biological sample are fluorescently labeled, but only the target cells are also magnetically labeled. In addition, non-magnetically labeled cells are imaged for viability in a modified slide configuration. The labeled sample, in a chamber or cuvet, is placed between two wedge-shaped magnets to selectively move the magnetically labeled cells to the observation surface of the cuvet.
    Type: Grant
    Filed: May 12, 2006
    Date of Patent: July 27, 2010
    Assignee: Veridex, LLC
    Inventors: Frank A. W. Coumans, Jan Greve, Frank P. Modica, Leon W. M. M. Terstappen, Arjan G. J. Tibbe, John A. Verrant
  • Patent number: 7666308
    Abstract: Apparatuses and methods for separating, immobilizing, and quantifying biological substances from within a fluid medium. Biological substances are observed by employing a vessel (6) having a chamber therein, the vessel comprising a transparent collection wall (5). A high internal gradient magnetic capture structure may be on the transparent collection wall (5), magnets (3) create an externally-applied force for transporting magnetically responsive material toward the transparent collection wall (5). V-shaped grooves on the inner surface of the viewing face of the chamber provide uniform. The invention is also useful in conducting quantitative analysis and sample preparation in conjunction with automated cell enumeration techniques.
    Type: Grant
    Filed: June 6, 2006
    Date of Patent: February 23, 2010
    Assignee: Veridex, LLC.
    Inventors: Tycho M. Scholtens, Leon L. M. M. Terstappen, Arjan G. J. Tibbe
  • Publication number: 20090061477
    Abstract: A system for enumeration of cells in fluids by image cytometry is described for assessment of target populations such as leukocyte subsets in different bodily fluids or bacterial contamination in environmental samples, food products and bodily fluids. Briefly, fluorescently labeled target cells are linked to magnetic particles or beads. In one embodiment, a small, permanent magnet is inserted directly into the chamber containing the labeled cells. The magnets are coated with PDMS silicone rubber to provide a smooth and even surface which allows imaging on a single focal plane. The magnet is removed from the sample and illuminated with fluorescent light emitted by the target cells captured by a CCD camera. In another embodiment, a floater having a permanent magnet allows the target cells to line up along a single imaging plane within the sample solution.
    Type: Application
    Filed: February 15, 2008
    Publication date: March 5, 2009
    Inventors: Arjan G.J. Tibbe, Leon W.M.M. Terstappen