Abstract: An apparatus for controlling assay steps using active flow components, the apparatus includes a microfluidic device containing a reservoir configured to contain a fluid and microfluidic channels connected to the reservoir, wherein the microfluidic channels are configured to create a microfluidic environment for an assay, active flow components, a sensor device configured to detect a sensed property of the fluid, and an external device connected to the microfluidic device including actuators, wherein the actuators are configured to connect the active flow components using a mechanical interface and initiate active flow processes corresponds to assay steps of the assay based on the active flow components, and a reading device configured to read the sensed property of the fluid from the sensor device, wherein the active flow components are configured to flow the fluid bi-directionally through the sensor device within the microfluidic environment based on active flow processes.

Abstract: An apparatus for performing microfluidic-based biochemical assays, the apparatus includes a microfluidic device, wherein the microfluidic device comprises at least a microfluidic feature comprising at least a reservoir configured to contain at least a fluid, and at least an alignment feature for positioning and attaching a sensor device, wherein the at least an alignment feature is not contacting the at least a microfluidic feature, at least a sensor device configured to be in sensed communication with the at least a fluid and detect at least a sensed property, and at least a flow component fluidically connected to the at least a microfluidic feature configured to flow the at least a fluid through the at least a sensor device.