Abstract: Aspects of the present disclosure include a buffer pack for dispensing buffer for sample preparation using a sample preparation cartridge. Also provided are a sealing plate configured for holding the buffer pack and a cap for actuating the buffer pack in the sample preparation cartridge. Aspects of the present disclosure include a sample preparation cartridge that includes the buffer pack and the sealing plate. Aspects of the present disclosure also include a sample preparation cartridge that includes the buffer pack, the sealing plate, and the cap. Methods for filling the buffer pack, assembling a cartridge for use in sample preparation, and using the cartridge for preparing a sample also provided.

Abstract: The methods for sample preparation disclosed herein utilize an air phase to reduce aqueous phase associated with magnetic particles bound to nucleic acids to decrease carryover of one or more contaminants, such as, cell debris, chaotropic agents, non-specifically attached molecules, and the like. This air-transfer step is improved by using a combination of a first population of magnetic particles and a second population of magnetic particles, where the first population of magnetic particles is capable of associating with nucleic acids, and the magnetic particles in the second population are at least two-times larger in size than the size of the magnetic particles in the first population. As discussed herein, the use of this second population of magnetic particles improves the transfer of the first population of magnetic particles by reducing loss of the first magnetic particles during the transfer. The sample preparation methods may be semi-automated or completely automated.

Abstract: Aspects of the present disclosure include a system for transporting a liquid from a chamber into one or more collection containers. The system may be semi-automatic or fully automatic. The system may be embodied in a sample preparation device, such as a cylindrical cartridge. The system includes a plunger chamber that creates negative pressure to fill the one or more collection containers. The plunger chamber is armed using a pivotable locking arm and a trigger coupled to the arm. Methods of using the system for transporting a liquid from a chamber into one or more collection containers is also provided.

Abstract: A microfluidic switching device that includes an upstream microfluidic channel configured to contain a liquid having particles therein and a plurality of outlet channels coupled to the upstream microfluidic channel at a junction. A dead-end side channel or LCAT is oriented generally perpendicular to the upstream microfluidic channel and coupled to the upstream microfluidic channel at the junction, the dead-end side channel having a gas contained therein. The device includes a transducer configured to apply an external source of acoustic energy. Actuation of the transducer effectuates symmetrical oscillation of a gas/liquid boundary at the junction. Preferably, the junction comprises a bifurcation with two outlets. Further, the LCAT has a leading edge and a trailing edge and wherein the trailing edge of the LCAT is substantially aligned with the bifurcation.

Abstract: A microfluidic switching device that includes an upstream microfluidic channel configured to contain a liquid having particles therein and a plurality of outlet channels coupled to the upstream microfluidic channel at a junction. A dead-end side channel or LCAT is oriented generally perpendicular to the upstream microfluidic channel and coupled to the upstream microfluidic channel at the junction, the dead-end side channel having a gas contained therein. The device includes a transducer configured to apply an external source of acoustic energy. Actuation of the transducer effectuates symmetrical oscillation of a gas/liquid boundary at the junction. Preferably, the junction comprises a bifurcation with two outlets. Further, the LCAT has a leading edge and a trailing edge and wherein the trailing edge of the LCAT is substantially aligned with the bifurcation.

Abstract: A system of metalization in an integrated polymer microsystem. A flexible polymer substrate is provided and conductive ink is applied to the substrate. In one embodiment the flexible polymer substrate is silicone. In another embodiment the flexible polymer substrate comprises poly(dimethylsiloxane).

Abstract: A stretchable electronic circuit or electronic device and a polymer-based process to produce a circuit or electronic device containing a stretchable conducting circuit. The stretchable electronic apparatus has a central longitudinal axis and the apparatus is stretchable in a longitudinal direction generally aligned with the central longitudinal axis. The apparatus comprises a stretchable polymer body and at least one circuit line operatively connected to the stretchable polymer body. The circuit line extends in the longitudinal direction and has a longitudinal component that extends in the longitudinal direction and has an offset component that is at an angle to the longitudinal direction. The longitudinal component and the offset component allow the apparatus to stretch in the longitudinal direction while maintaining the integrity of the circuit line.

Abstract: A method of metalization of an integrated microsystem. The method comprises providing a substrate and applying a conductive material to the substrate by taking up small aliquots of conductive material and releasing the conductive material onto the substrate to produce a circuit component.

Abstract: A system of metalization in an integrated polymer microsystem. A flexible polymer substrate is provided and conductive ink is applied to the substrate. In one embodiment the flexible polymer substrate is silicone. In another embodiment the flexible polymer substrate comprises poly(dimethylsiloxane).

Abstract: A stretchable electronic circuit or electronic device and a polymer-based process to produce a circuit or electronic device containing a stretchable conducting circuit. The stretchable electronic apparatus has a central longitudinal axis and the apparatus is stretchable in a longitudinal direction generally aligned with the central longitudinal axis. The apparatus comprises a stretchable polymer body and at least one circuit line operatively connected to the stretchable polymer body. The circuit line extends in the longitudinal direction and has a longitudinal component that extends in the longitudinal direction and has an offset component that is at an angle to the longitudinal direction. The longitudinal component and the offset component allow the apparatus to stretch in the longitudinal direction while maintaining the integrity of the circuit line.

Abstract: A system of metalization in an integrated polymer microsystem. A flexible polymer substrate is provided and conductive ink is applied to the substrate. In one embodiment the flexible polymer substrate is silicone. In another embodiment the flexible polymer substrate comprises poly(dimethylsiloxane).