Abstract: The invention relates to a chromatographic column for separating antigen-antibody complexes from the free antigens not bonded to the antibodies and/or for complete bonding of all marked substances in the immunological determination of antigens or haptens by radio-immunological, luminescence-immunological, fluorescence-immunological or enzyme-immunological determination methods in which a crepe-like tightly rolled filter paper of high degree of purity, in particular of regenerate cellulose, is pressed into a water-proof and water-tight envelope as nonpolar column material. According to the invention the chromatographic upright column comprises an upper reaction portion chargeable from above and a lower separation and measuring portion which are separated from each other by a perforable bottom, the column being fastened from below on the perforable bottom of the column.