Abstract: A capture construct for capturing a plurality of analytes of a biological sample includes a nanostructure backbone, at least a first orientation indicator and a second orientation indicator, and at least a first plurality of capture regions on the nanostructure backbone. Each capture region includes at least one affinity capture reagent configured to capture one of the plurality of analytes.

Abstract: A laser scanning microscope includes a light source configured to emit an illumination light beam. The illumination light beam has a transverse light intensity profile comprising an intensity minimum. The laser scanning microscope further includes a scanning device configured to scan the illumination light beam along a closed trajectory in a target area of a specimen, and a detector configured to detect fluorescence light emitted by a fluorophore within the target area of the specimen. The fluorophore is excited by the illumination light beam. The laser scanning microscope further includes a processor configured to determine an intensity distribution of the fluorescence light as a function of time and to determine a position of the fluorophore within the target area based on the intensity distribution of the fluorescence light.

Abstract: An optical arrangement has an optical beam path for illuminating a sample space with a sequence of laser light pulses generated in a laser cycle, the optical arrangement. At least one laser light source is configured to generate the sequence of laser light pulses along the optical beam path. A wavelength-selective pulse picker is situated in the optical beam path and has, in a predefined illumination clock timing synchronizable with the laser light pulses, an open state in which the pulse picker is light-transparent to at least one laser light pulse towards the sample space. The open state has at least two different transmission states which differ with regard to their respective transmission spectrums, and wherein the two transmission states are switchable on and/or off independently of one another.

Abstract: The invention relates to a microscope having an objective that focuses illuminating light to an illuminating light focus, and having a light-guiding fiber which transports the illuminating light and at whose end is arranged a fiber coupler that couples the illuminating light out of the light-guiding fiber and generates a preferably collimated illuminating light bundle. An element for modifying the shape of the illuminating light focus, which is prealigned relative to the illuminating light bundle to be coupled out, is arranged in or on the fiber coupler.

Abstract: The aim of the invention is an optical device for a scanning microscope, said device enabling the focusing of a light beam largely independent of wavelengths; and thus high-resolution microscopy, in particular STED microscopy, in a wider wavelength spectrum is facilitated. At least two phase filters lie on a support. Advantageously, the support is a filter wheel or a filter slider which can be introduced into the beam path of the light beam, said beam path preferably being the beam path of the stimulating light beam in an STED microscope. Several phase filters preferablylie on the support in the shape of a matrix. The support is designed as a glass substrate on which each phase filter is applied. To achieve said aim, another position is additionally found on the support for adjustment purposes, wherein the wavefront of the light is not influenced when it passes through said position, that is, the position is an empty position on which no phase filter is found.