Abstract: Isolated polypeptides having 5?-kinase and/or 3?-phosphatase activity and temperature optimum of at least 60° C. are described. The invention also relates to isolated nucleic acids encoding the polypeptides, nucleic acid constructs and host cells comprising the nucleic acid sequences as well as methods using the polypeptides and kits for practicing the methods.

Abstract: The invention provides novel strand displacement DNA polymerases which can be used in a rapid and efficient strand displacement amplification reactions. The polymerases are significantly more thermostable than prior art polymerase and retain high activity at elevated temperatures. Also disclosed are genes encoding the polymerases and vectors comprising the genes. Representative polymerases of the invention are obtainable from bacterial strains of the species Thermus antranikianii and Thermus brockianus and also from environmental samples with isolation of the source species.

Abstract: An RNA ligase derived from bacteriophage TS2126 which infects Thermus scotoductus, nucleic acids comprising nucleotide sequences of open reading frame (ORF) and polypeptides encoded by the nucleic acids, are described.

Abstract: An RNA ligase derived from bacteriophage TS2126 which infects Thermus scotoductus, nucleic acids comprising nucleotide sequences of open reading frame (ORF) and polypeptides encoded by the nucleic acids, are described.

Abstract: A novel bacteriophage RM 378 of Rhodothermus marinus, the nucleic acids of its genome, nucleic acids comprising nucleotide sequences of open reading frames (ORFs) of its genome, and polypeptides encoded by the nucleic acids, are described.

Abstract: The present invention provides a method that can facilitate structure determination of target proteins by x-ray crystallography. It is a method of rational crystallization of members of a target protein family obtained through specific amplification of corresponding genes from natural diversity. The method makes broad biodiversity accessible through sampling and ecological enrichment of diverse high-temperature ecosystems containing thermophilic microorganisms including uncultivable and previously unknown organisms. The method provides means to circumvent many potential problems and bottlenecks in crystal structure determination by selection of suitable proteins directly from nature. The invention combines methods of accessing and screening vast natural diversity and the inherent suitability of thermostable proteins for crystallization in order to maximize probability of successful structure determination.

Abstract: The activity and functional properties of novel thermostable RNA ligases isolated from thermophilic bacteriophages are described. Also described are methods of using these thermostable RNA ligases for various applications including nucleotide labeling, oligonucleotide synthesis, gene synthesis, gene amplification and amplification of mRNA and synthesis of cDNA.

Abstract: The crystal of a hyperthermostable cellulase from Rhodothermus marinus and the three-dimensional structure of the enzyme are provided. The invention further provides procedures for the identification of structural features that are important for thermostability of the enzyme. Methods based thereon to rationally modify proteins structurally related to R. marinus are disclosed, in particular, methods for increased thermostability are provided. Modified proteins are provided, including modified variants of cellulase from Trichoderma reesei.

Abstract: A novel bacteriophage RM 378 of Rhodothermus marinus, the nucleic acids of its genome, nucleic acids comprising nucleotide sequences of open reading frames (ORFs) of its genome, and polypeptides encoded by the nucleic acids, are described.

Abstract: A novel bacteriophage RM 378 of Rhodothermus marinus, the nucleic acids of its genome, nucleic acids comprising nucleotide sequences of open reading frames (ORFs) of its genome, and polypeptides encoded by the nucleic acids, are described.

Abstract: A novel bacteriophage RM 378 of Rhodothermus marinus, the nucleic acids of its genome, nucleic acids comprising nucleotide sequences of open reading frames (ORFs) of its genome, and polypeptides encoded by the nucleic acids, are described.

Abstract: A novel bacteriophage RM 378 of Rhodothermus marinus, the nucleic acids of its genome, nucleic acids comprising nucleotide sequences of open reading frames (ORFs) of its genome, and polypeptides encoded by the nucleic acids, are described.

Abstract: A novel bacteriophage RM 378 of Rhodothermus marinus, the nucleic acids of its genome, nucleic acids comprising nucleotide sequences of open reading frames (ORFs) of its genome, and polypeptides encoded by the nucleic acids, are described.

Abstract: A novel bacteriophage RM 378 of Rhodothermus marinus, the nucleic acids of its genome, nucleic acids comprising nucleotide sequences of open reading frames (ORFs) of its genome, and polypeptides encoded by the nucleic acids, are described.

Abstract: A novel bacteriophage RM 378 of Rhodothermus marinus, the nucleic acids of its genome, nucleic acids comprising nucleotide sequences of open reading frames (ORFs) of its genome, and polypeptides encoded by the nucleic acids, are described.