Patents by Inventor Arren Bar-Even
Arren Bar-Even has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11976310Abstract: The present disclosure relates to a method for the incorporation of formaldehyde into biomass comprising the following enzymatically catalyzed steps: (1) condensation of pyruvate with formaldehyde into 4-hydroxy-2-oxobutanoic acid (HOB); (2) amination of the thus produced 4-hydroxy-2-oxobutanoic acid (HOB) to produce homoserine; (3) conversion of thus produced homoserine to threonine; (4) conversion of the thus produced threonine into glycine and acetaldehyde or acetyl-CoA; (5) condensation of the thus produced glycine with formaldehyde to produce serine; and (6) conversion of the thus produced serine to produce pyruvate, wherein said pyruvate can then be used as a substrate in step (1). The disclosure also relates to enzymes for catalyzing the corresponding enzymatic reactions and recombinant microorganisms which express the enzymes for catalyzing the corresponding enzymatic reactions.Type: GrantFiled: February 16, 2021Date of Patent: May 7, 2024Inventors: Arren Bar-Even, Hai He, Philippe Marliere
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Publication number: 20240117386Abstract: A recombinant bacteria which is genetically modified to express formate dehydrogenase (FDH), phosphoribulokinase (prk) and Ribulose-Bisphosphate Carboxylase/oxygenase (RuBisCo) is disclosed. The bacteria may be modified to be autotrophic.Type: ApplicationFiled: October 22, 2020Publication date: April 11, 2024Applicant: Yeda Research and Development Co. Ltd.Inventors: Ron MILO, Shmuel GLEIZER, Niv ANTONOVSKY, Elad NOOR, Arren BAR-EVEN, Yehudit ZOHAR, Roee BEN NISSAN, Elad HERZ, Yinon Moise BAR-ON
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Publication number: 20230212622Abstract: Described is a method for the incorporation of formaldehyde into biomass comprising the following enzymatically catalyzed steps (1) condensation of pyruvate with formaldehyde into 4-hydroxy-2-oxobutanoic acid (HOB); (2) amination of the thus produced 4-hydroxy-2-oxobutanoic acid (HOB) to produce homoserine; (3) conversion of thus produced homoserine to threonine; (4) conversion of the thus produced threonine into glycine and acetaldehyde or acetyl-CoA; (5) condensation of the thus produced glycine with formaldehyde to produce serine; and (6) conversion of the thus produced serine to produce pyruvate, wherein said pyruvate can then be used as a substrate in step (1).Type: ApplicationFiled: February 16, 2021Publication date: July 6, 2023Inventors: Arren BAR-EVEN, Hai HE, Philippe MARLIÈRE
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Publication number: 20220348935Abstract: The present invention relates to a genetically engineered microorganism expressing (i) formate tetrahydrofolate (THF) ligase, methenyi-THF cyclohydrolase and methylene-THF dehydrogenase, (ii) the enzymes of the glycine cleavage system (GCS), (iii) serine deaminase and serine hydroxymethyltransferase (SHMT), (iv) an enzyme increasing the availability of NADPH, and (v) optionally formate dehydrogenase (FDH), and wherein the genetically engineered microorganism has been genetically engineered to express at least one of the enzymes of (i) to (v), wheren said enzyme is not expressed by the corresponding microorganism that has been used to prepare the genetically engineered microorganism, and wherein the enzymes of (i) to (v) are genomically expressed.Type: ApplicationFiled: December 10, 2020Publication date: November 3, 2022Inventors: Arren BAR-EVEN (deceased), Oren YISHAI, Seohyoung KIM
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Patent number: 10781456Abstract: The present invention relates to an organism, a tissue, a cell or an organelle expressing enzymes which allow the conversion of 2-phosphoglycolate (2-PG; also known as glycolate 2-phosphate,) into an intermediate compound of the Calvin-Benson-Bassham Cycle (CBBC) without releasing CO2. The organism, tissue, cell or organelle of the invention may be genetically engineered, transgenic and/or transplastomic so as to express at least one enzyme which is involved in this conversion. The present invention further relates to an organism, tissue, cell or organelle which comprises/expresses at least one enzyme which is involved in this conversion. The present invention further relates to a method for producing an organism, tissue, cell or organelle of the invention. The present invention further relates to a method of enzymatically converting 2-PG into an intermediate compound of the CBBC without releasing CO2.Type: GrantFiled: June 22, 2016Date of Patent: September 22, 2020Inventors: Arren Bar-Even, Tobias Erb, Steffen Lindner, Philippe Marliere, Dan S. Tawfik
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Patent number: 10377994Abstract: An isolated microorganism that expresses enzymes of the reductive glycine pathway is disclosed. The microorganism is capable of converting formate to pyruvate or glycerate via the formation of glycine and serine. Methods of generating same are further described.Type: GrantFiled: October 17, 2018Date of Patent: August 13, 2019Assignee: Yeda Research and Developmetn Co. Ltd.Inventors: Arren Bar-Even, Ron Milo, Elad Noor, Oren Yishai
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Publication number: 20190211342Abstract: A microorganism which is genetically modified so that it produces a first essential biomass precursor by metabolizing CO2 using a recombinant carbon fixation enzyme is disclosed. The microorganism produces a second biomass precursor by metabolizing an organic carbon source and not by metabolizing CO2. The microorganism does not use the organic carbon source for producing the first essential biomass precursor.Type: ApplicationFiled: March 25, 2019Publication date: July 11, 2019Applicant: Yeda Research and Development Co. Ltd.Inventors: Ron MILO, Niv ANTONOVSKY, Elad NOOR, Arren BAR-EVEN, Yehudit ZOHAR, Lior ZELCBUCH, Shmuel GLEIZER, Shira AMRAM
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Patent number: 10294482Abstract: A microorganism which is genetically modified so that it produces a first essential biomass precursor by metabolizing CO2 using a recombinant carbon fixation enzyme is disclosed. The microorganism produces a second biomass precursor by metabolizing an organic carbon source and not by metabolizing CO2. The microorganism does not use the organic carbon source for producing the first essential biomass precursor.Type: GrantFiled: May 21, 2015Date of Patent: May 21, 2019Assignee: Yeda Research and Development Co. Ltd.Inventors: Ron Milo, Niv Antonovsky, Elad Noor, Arren Bar-Even, Yehudit Zohar, Lior Zelcbuch, Shmuel Gleizer, Shira Amram
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Publication number: 20190032027Abstract: An isolated microorganism that expresses enzymes of the reductive glycine pathway is disclosed. The microorganism is capable of converting formate to pyruvate or glycerate via the formation of glycine and serine. Methods of generating same are further described.Type: ApplicationFiled: October 17, 2018Publication date: January 31, 2019Applicant: Yeda Research and Development Co. Ltd.Inventors: Arren Bar-Even, Ron Milo, Elad Noor, Oren Yishai
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Patent number: 10155933Abstract: An isolated microorganism that expresses enzymes of the reductive glycine pathway is disclosed. The microorganism is capable of converting formate to pyruvate or glycerate via the formation of glycine and serine. Methods of generating same are further described.Type: GrantFiled: July 29, 2013Date of Patent: December 18, 2018Assignee: Yeda Research and Development Co., Ltd.Inventors: Arren Bar-Even, Ron Milo, Elad Noor, Oren Yishai
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Patent number: 10077437Abstract: A system for carbon fixation is provided. The system comprises enzymes which catalyze reactions of a carbon fixation pathway, wherein at least one of the reactions of the carbon fixation pathway is a carboxylation reaction, wherein products of the reactions of the carbon fixation pathway comprise oxaloacetate and malonyl-CoA, wherein an enzyme which performs the carboxylation reaction is selected from the group consisting of phophoenolpyruvate (PEP) carboxlase, pyruvate carboxylase and acetyl-CoA carboxylase and wherein an export product of the carbon fixation pathway is glyoxylate. Additional carbon fixation pathways are also provided and methods of generating same.Type: GrantFiled: June 22, 2016Date of Patent: September 18, 2018Assignee: Yeda Research and Development Co. Ltd.Inventors: Ron Milo, Arren Bar-Even, Elad Noor
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Patent number: 10053712Abstract: An isolated microorganism is disclosed being genetically modified to express pyruvate formate lyase (PFL) or 2-ketobutyrate formate lyase, wherein acetyl-CoA of the microorganism is converted to pyruvate in the presence of formate in a single step reaction, wherein the net flux of the reaction is in the direction of pyruvate synthesis. Uses of the microorganism and products comprising same are also disclosed.Type: GrantFiled: December 10, 2014Date of Patent: August 21, 2018Assignee: Yeda Research and Development Co. Ltd.Inventors: Arren Bar-Even, Ron Milo, Elad Noor, Lior Zelcbuch
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Publication number: 20180223302Abstract: The present invention relates to an organism, a tissue, a cell or an organelle expressing enzymes which allow the conversion of 2-phosphoglycolate (2-PG; also known as glycolate 2-phosphate) into an intermediate compound of the Calvin-Benson-Bassham Cycle (CBBC) without releasing CO2. The organism, tissue, cell or organelle of the invention may be genetically engineered, transgenic and/or transplastomic so as to express at least one enzyme which is involved in this conversion. The present invention further relates to an organism, tissue, cell or organelle which comprises/expresses at least one enzyme which is involved in this conversion. The present invention further relates to a method for producing an organism, tissue, cell or organelle of the invention. The present invention further relates to a method of enzymatically converting 2-PG into an intermediate compound of the CBBC without releasing CO2.Type: ApplicationFiled: June 22, 2016Publication date: August 9, 2018Applicants: Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V., Yeda Research and Development Co. Ltd., at the Weizmann Institute of ScienceInventors: Arren BAR-EVEN, Tobias ERB, Steffen LINDNER, Philippe MARLIERE, Dan S. TAWFIK
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Publication number: 20170183665Abstract: A microorganism which is genetically modified so that it produces a first essential biomass precursor by metabolizing CO2 using a recombinant carbon fixation enzyme is disclosed. The microorganism produces a second biomass precursor by metabolizing an organic carbon source and not by metabolizing CO2. The microorganism does not use the organic carbon source for producing the first essential biomass precursor.Type: ApplicationFiled: May 21, 2015Publication date: June 29, 2017Inventors: Ron MILO, Niv ANTONOVSKY, Elad NOOR, Arren BAR-EVEN, Yehudit ZOHAR, Lior ZELCBUCH, Shmuel GLEIZER, Shira AMRAM
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Publication number: 20170081682Abstract: An isolated microorganism is disclosed being genetically modified to express pyruvate formate lyase (PFL) or 2-ketobutyrate formate lyase, wherein acetyl-CoA of the microorganism is converted to pyruvate in the presence of formate in a single step reaction, wherein the net flux of the reaction is in the direction of pyruvate synthesis. Uses of the microorganism and products comprising same are also disclosed.Type: ApplicationFiled: December 10, 2014Publication date: March 23, 2017Applicant: Yeda Research and Development Co. Ltd.Inventors: Arren BAR-EVEN, Ron MILO, Elad NOOR, Lior ZELCBUCH
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Publication number: 20160298103Abstract: A system for carbon fixation is provided. The system comprises enzymes which catalyze reactions of a carbon fixation pathway, wherein at least one of the reactions of the carbon fixation pathway is a carboxylation reaction, wherein products of the reactions of the carbon fixation pathway comprise oxaloacetate and malonyl-CoA, wherein an enzyme which performs the carboxylation reaction is selected from the group consisting of phophoenolpyruvate (PEP) carboxlase, pyruvate carboxylase and acetyl-CoA carboxylase and wherein an export product of the carbon fixation pathway is glyoxylate. Additional carbon fixation pathways are also provided and methods of generating same.Type: ApplicationFiled: June 22, 2016Publication date: October 13, 2016Applicant: Yeda Research and Development Co. Ltd.Inventors: Ron MILO, Arren BAR-EVEN, Elad NOOR
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Patent number: 9410131Abstract: A system for carbon fixation is provided. The system comprises enzymes which catalyze reactions of a carbon fixation pathway, wherein at least one of the reactions of the carbon fixation pathway is a carboxylation reaction, wherein products of the reactions of the carbon fixation pathway comprise oxaloacetate and malonyl-CoA, wherein an enzyme which performs the carboxylation reaction is selected from the group consisting of phophoenolpyruvate (PEP) carboxlase, pyruvate carboxylase and acetyl-CoA carboxylase and wherein an export product of the carbon fixation pathway is glyoxylate. Additional carbon fixation pathways are also provided and methods of generating same.Type: GrantFiled: February 10, 2011Date of Patent: August 9, 2016Assignee: Yeda Research and Development Co. Ltd.Inventors: Ron Milo, Arren Bar-Even, Elad Noor
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Publication number: 20150218528Abstract: An isolated microorganism that expresses enzymes of the reductive glycine pathway is disclosed. The microorganism is capable of converting formate to pyruvate or glycerate via the formation of glycine and serine. Methods of generating same are further described.Type: ApplicationFiled: July 29, 2013Publication date: August 6, 2015Inventors: Arren Bar-Even, Ron Milo, Elad Noor, Oren Yishai
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Publication number: 20120301947Abstract: A system for carbon fixation is provided. The system comprises enzymes which catalyze reactions of a carbon fixation pathway, wherein at least one of the reactions of the carbon fixation pathway is a carboxylation reaction, wherein products of the reactions of the carbon fixation pathway comprise oxaloacetate and malonyl-CoA, wherein an enzyme which performs the carboxylation reaction is selected from the group consisting of phophoenolpyruvate (PEP) carboxlase, pyruvate carboxylase and acetyl-CoA carboxylase and wherein an export product of the carbon fixation pathway is glyoxylate. Additional carbon fixation pathways are also provided and methods of generating same.Type: ApplicationFiled: February 10, 2011Publication date: November 29, 2012Applicant: Yeda Research and Development Co. Ltd.Inventors: Ron Milo, Arren Bar-Even, Elad Noor