Abstract: The present disclosure relates to methods for expressing human insulin derivative in which the motif Leucine-Proline-X-Threonine appears at end of B-chain, where X is any amino acid residue. Said human insulin derivative is expressed using insulin derivative peptide and sortase. The method is to translate a nucleotide sequence encoding a single chain peptide into a precursor of human insulin derivative and combine the single chain peptide with sortase to convert a human insulin derivative precursor into a fully folded, biologically active human insulin derivative.

Abstract: The present invention relates to insulin derivatives which, in comparison to insulin glulisine and similar derivatives, has a simplified process for synthesis. In particular, the present invention relates to insulin derivatives or physiologically tolerable salts thereof in which the motif Leucine-Proline-X-Threonine appears at the end of the B-chain, where X is any amino acid residue. Due to the nature of the process, the A-chain and B-chain must begin with a Glycine amino acid residue.

Abstract: The present invention relates to insulin derivatives which, in comparison to insulin glulisine and similar derivatives, has a simplified process for synthesis. In particular, the present invention relates to insulin derivatives or physiologically tolerable salts thereof in which the motif Leucine-Proline-X-Threonine appears at the end of the B-chain, where X is any amino acid residue. Due to the nature of the process, the A-chain and B-chain must begin with a Glycine amino acid residue.

Abstract: The present invention relates to insulin derivatives which, in comparison to insulin glulisine and similar derivatives, has a simplified process for synthesis. In particular, the present invention relates to insulin derivatives or physiologically tolerable salts thereof in which the motif Leucine-Proline-X-Threonine appears at the end of the B-chain, where X is any amino acid residue. Due to the nature of the process, the A-chain and B-chain must begin with a Glycine amino acid residue.

Abstract: Described herein are various principles related to collecting and analyzing fertility data for female humans. The underlying concept is that a woman's hormones fluctuate throughout the menstrual cycle, affecting optimal exercise routines and general health practices. A dedicated sensor may be used to collect fertility data, or an estimate may be derived from the individual's menstrual history. Once collected or estimated, the fertility data is factored with other variables to determine the optimal exercise routine or general health habits for the woman. The recommendations are communicated to the woman, who may provide feedback to further improve future recommendations.