Abstract: We have successfully sequenced and cloned the gene expressing the Major Surface Protein 5 (MSP5) of A. phagocytophilum. The recombinant MSP5 (rMSP5) protein has been tested using sera from humans and dogs infected with A. phacytophilum. The polypeptide has been found to be immunogenic and useful as a diagnostic test antigen. The polypeptide antigen of the subject invention can provide the basis of a diagnostic assay that would allow the rapid, in-house, laboratory diagnosis of infection with A. phagocytophilum using a sample (e.g., serum, plasma, or whole blood) from an infected human or animal. Additionally, the subject invention provides methods of detecting the presence of A. phagocytophilum in biological or environmental samples utilizing antibodies provided by the subject invention.

Abstract: A bulb assembly, including a faceplate panel portion, and a mount assembly, comprising a stem and a multi-beam electron gun assembly, are positioned in axial alignment on respective central longitudinal axes. A reference plane which contains the central longitudinal axis of the bulb assembly and a major axis of the panel portion, is established. An orientation plane is then defined with reference to the structure of the electron gun assembly. The orientation plane is parallel to the central longitudinal axis of the mount assembly and includes two reference points on the structures of the electron gun assembly. The mount assembly is then rotated with respect to the bulb assembly on the coincident longitudinal axes until the orientation plane is at a prescribed angle with respect to the reference plane as optically indicated by the relative position of the two reference points with respect to each other.