Patents by Inventor Arthur Keith Turner
Arthur Keith Turner has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20220403373Abstract: Described is a process for producing a mutant bacterium which exhibits improved survival and/or growth under a selected growth condition, the process comprising the steps of: (a) generating a pool of mutant bacteria by transposon mutagenesis with an activating transposon (TnA), wherein the TnA comprises a promoter capable of increasing transcription of a gene at or near its insertion site; (b) growing bacteria from the mutant pool under the selected growth condition and under one or more reference conditions to produce two or more test cultures; and (c) comparing the distribution of TnA insertions between test cultures to identify a first class of genes which are disadvantageous for growth and/or survival under the selected growth condition and a second class of genes which are advantageous for growth and/or survival under the selected growth condition.Type: ApplicationFiled: February 24, 2022Publication date: December 22, 2022Inventors: David Hugh Williams, Arthur Keith Turner, John Richard Wain
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Publication number: 20220186211Abstract: Disclosed are methods related to identifying an essential gene which serves as an antibiotic target in a bacterium.Type: ApplicationFiled: July 20, 2017Publication date: June 16, 2022Applicant: DISCUVA LIMITEDInventors: David Hugh Williams, Arthur Keith Turner
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Patent number: 11359195Abstract: Disclosed are methods related to identifying an essential gene which serves as an antibiotic target in a bacterium.Type: GrantFiled: July 20, 2017Date of Patent: June 14, 2022Assignee: DISCUVA LIMITEDInventors: David Hugh Williams, Arthur Keith Turner
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Publication number: 20180135041Abstract: Described is a process for producing a mutant bacterium which exhibits improved survival and/or growth under a selected growth condition, the process comprising the steps of: (a) generating a pool of mutant bacteria by transposon mutagenesis with an activating transposon (TnA), wherein the TnA comprises a promoter capable of increasing transcription of a gene at or near its insertion site; (b) growing bacteria from the mutant pool under the selected growth condition and under one or more reference conditions to produce two or more test cultures; and (c) comparing the distribution of TnA insertions between test cultures to identify a first class of genes which are disadvantageous for growth and/or survival under the selected growth condition and a second class of genes which are advantageous for growth and/or survival under the selected growth condition.Type: ApplicationFiled: November 20, 2017Publication date: May 17, 2018Inventors: David Hugh Williams, Arthur Keith Turner, John Richard Wain
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Publication number: 20180010122Abstract: Disclosed are methods related to identifying an essential gene which serves as an antibiotic target in a bacterium.Type: ApplicationFiled: July 20, 2017Publication date: January 11, 2018Applicant: DISCUVA LIMITEDInventors: David Hugh Williams, Arthur Keith Turner
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Patent number: 9745572Abstract: Provided are methods for identifying differentially represented genes, the method comprising the steps of generating a pool of mutant bacteria by transposon mutagenesis with an activating transposon (TnA), wherein the TnA comprises a promoter such that transposon insertion into bacterial DNA increases the transcription of a gene at or near the insertion site; growing bacteria from the mutant pool in the presence of different amounts of said antibiotic to produce two or more test cultures; and comparing the distribution of TnA insertions between test cultures.Type: GrantFiled: November 4, 2013Date of Patent: August 29, 2017Assignee: DISCUVA LIMITEDInventors: David Hugh Williams, Arthur Keith Turner
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Patent number: 9493770Abstract: Provided herein are methods, and related compositions for identifying a putative essential gene which serves as an antibiotic target in a bacterium, the method, in some embodiments, comprising (a) generating an antibiotic resistant mutant of the bacterium by a method comprising the step of selecting for growth in the presence of an antibiotic to produce an antibiotic resistant mutant clone (AbR mutant); (b) transforming the AbR mutant with one or more essential genes of the bacterium and a transposon which insertionally inactivates bacterial DNA to produce a pool of transposon mutants which are merodiploid for the one or more essential genes; (c) growing bacteria from the merodiploid pool in the presence of different amounts of the antibiotic to produce two or more test cultures; and (d) comparing the distribution of transposon insertions between the test cultures to identify a putative essential gene serving as a target of the antibiotic in the bacterium.Type: GrantFiled: November 4, 2013Date of Patent: November 15, 2016Assignee: DISCUVA LIMITEDInventors: David Hugh Williams, Arthur Keith Turner
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Publication number: 20150307873Abstract: Described is a process for producing a mutant bacterium which exhibits improved survival and/or growth under a selected growth condition, the process comprising the steps of: (a) generating a pool of mutant bacteria by transposon mutagenesis with an activating transposon (TnA), wherein the TnA comprises a promoter capable of increasing transcription of a gene at or near its insertion site; (b) growing bacteria from the mutant pool under the selected growth condition and under one or more reference conditions to produce two or more test cultures; and (c) comparing the distribution of TnA insertions between test cultures to identify a first class of genes which are disadvantageous for growth and/or survival under the selected growth condition and a second class of genes which are advantageous for growth and/or survival under the selected growth condition.Type: ApplicationFiled: May 6, 2015Publication date: October 29, 2015Applicant: Bactevo Ltd.Inventors: David Hugh Williams, Arthur Keith Turner, John Richard Wain
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Publication number: 20140141979Abstract: Disclosed is a method for identifying an essential gene which serves as an antibiotic target in a bacterium, the method comprising the steps of: (a) generating a pool of mutant bacteria by transposon mutagenesis with an activating transposon (TnA), wherein the TnA comprises a promoter such that transposon insertion into bacterial DNA increases the transcription of a gene at or near the insertion site; (b) growing bacteria from the mutant pool in the presence of different amounts of said antibiotic to produce two or more test cultures; and (c) comparing the distribution of TnA insertions between test cultures to identify a putative essential gene serving as a target of said antibiotic in said bacterium.Type: ApplicationFiled: November 4, 2013Publication date: May 22, 2014Applicant: DISCUVA LIMITEDInventors: DAVID HUGH WILLIAMS, Arthur Keith Turner
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Publication number: 20140135234Abstract: Disclosed is a method for identifying an essential gene which serves as an antibiotic target in a bacterium, the method comprising the steps of: (a) generating an antibiotic resistant mutant of said bacterium by a method comprising the step of selecting for growth in the presence of said antibiotic to produce an antibiotic resistant mutant clone (AbR mutant); (b) transforming the AbR mutant with: (i) one or more essential genes of said bacterium; and (ii) a transposon which insertionally inactivates bacterial DNA, to produce a pool of transposon mutants which are merodiploid for said one or more essential genes; (c) growing bacteria from the merodiploid pool in the presence of different amounts of said antibiotic to produce two or more test cultures; and (d) comparing the distribution of transposon insertions between test cultures to identify a putative essential gene serving as a target of said antibiotic in said bacterium.Type: ApplicationFiled: November 4, 2013Publication date: May 15, 2014Applicant: DISCUVA LIMITEDInventors: DAVID HUGH WILLIAMS, Arthur Keith Turner
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Patent number: 8318148Abstract: The invention provides strains of bacteria, especially enterotoxigenic E. coli, attenuated by mutations in the genes encoding enterotoxins (LT, ST, EAST1) and optionally further attenuated by deletion of additional chromosomal genes. In addition the invention provides strains of attenuated bacteria expressing immunogenic but non-toxic variants of one or more of these enterotoxins. These bacteria are useful as a vaccine against diarrhoeal disease.Type: GrantFiled: March 28, 2011Date of Patent: November 27, 2012Assignee: Acambis Research LimitedInventors: Arthur Keith Turner, Judith Greenwood, Jonathan Clive Stephens, Juliet Claire Beavis, Michael James Darsley
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Publication number: 20110200638Abstract: A bacterial cell which expresses three or more coli surface (CS) antigens and methods of making such a cell. The cell is useful in making vaccines against diarrhea.Type: ApplicationFiled: April 7, 2011Publication date: August 18, 2011Applicant: ACAMBIS RESEARCH LIMITEDInventors: Arthur Keith Turner, Judith Greenwood, Jonathan Clive Stephens, Juliet Claire Beavis, Michael James Darsley
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Publication number: 20110189225Abstract: The invention provides strains of bacteria, especially enterotoxigenic E. coli, attenuated by mutations in the genes encoding enterotoxins (LT, ST, EAST1) and optionally further attenuated by deletion of additional chromosomal genes. In addition the invention provides strains of attenuated bacteria expressing immunogenic but non-toxic variants of one or more of these enterotoxins. These bacteria are useful as a vaccine against diarrhoeal disease.Type: ApplicationFiled: March 28, 2011Publication date: August 4, 2011Applicant: ACAMBIS RESEARCH LIMITEDInventors: ARTHUR KEITH TURNER, JUDITH GREENWOOD, JONATHAN CLIVE STEPHENS, JULIET CLAIRE BEAVIS, MICHAEL JAMES DARSLEY
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Patent number: 7951361Abstract: A bacterial cell which expresses three or more coli surface (CS) antigens and methods of making such a cell. The cell is useful in making vaccines against diarrhea.Type: GrantFiled: September 11, 2002Date of Patent: May 31, 2011Assignee: Acambis Research LimitedInventors: Arthur Keith Turner, Judith Greenwood, Jonathan Clive Stephens, Juliet Claire Beavis, Michael James Darsley
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Patent number: 7943122Abstract: The invention provides strains of bacteria, especially enterotoxigenic E. coli, attenuated by mutations in the genes encoding enterotoxins (LT, ST, EAST1) and optionally further attenuated by deletion of additional chromosomal genes. In addition the invention provides strains of attenuated bacteria expressing immunogenic but non-toxic variants of one or more of these enterotoxins. These bacteria are useful as a vaccine against diarrhoeal disease.Type: GrantFiled: September 11, 2002Date of Patent: May 17, 2011Assignee: Acambis Research LimitedInventors: Arthur Keith Turner, Judith Greenwood, Jonathan Clive Stephens, Juliet Claire Beavis, Michael James Darsley
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Publication number: 20040253710Abstract: A bacterial cell which expresses three or more coli surface (CS) antigens and methods of making such a cell. The cell is useful in making vaccines against diarrhoea.Type: ApplicationFiled: August 5, 2004Publication date: December 16, 2004Inventors: Arthur Keith Turner, Judith Greenwood, Jonathan Clive Stephens, Juliet Claire Beavis, Michael James Darsley
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Publication number: 20030170264Abstract: The invention relates to a vaccine comprising a pharmaceutically acceptable carrier or diluent and a bacterium, wherein the bacterium contains (i) a DNA sequence encoding a site-specific recombinase, and (ii) a plasmid comprising a recognition element for the recombinase and a DNA sequence encoding a heterologous polypeptide. The recombinase stabilises inheritance of the plasmid and thereby prevents the bacterium from losing the ability to express the heterologous polypeptide.Type: ApplicationFiled: April 3, 2003Publication date: September 11, 2003Inventors: Arthur Keith Turner, Jonathan Clive Stephens