Abstract: A microscope slide assembly and a method of preparing such which includes an optically clear glass slide having a controlled thickness fluorocarbon layer coated on one planar surface. The layer is provided with a multiplicity of openings to form wells on the glass slide. An agar solution may be incorporated within the wells to provide an adhesive layer. In addition, a particular antigen may be layered on top of the agar composition to provide a completed assembly wherein only a patient's serum is needed to process a microscopic examination. For use in fluorescent microscopy, a film body grade Teflon containing an adhesive is used to provide the uniform fluorocarbon layer. The microscopic assembly may be enclosed within an environment isolation container to prevent contamination of the assembly from the ambient surroundings when the antigen is combined with the agar solution.