Abstract: Methods and compositions comprising an expression construct and a suppressor of posttranscriptional gene silencing construct are described. The expression construct and suppressor construct may comprise a viral amplicon. The expression construct may comprise fusing the target gene to the 3? and/or 5? end of a gene encoding a transit peptide sequence or a signaling peptide sequences. The transit or signal peptide sequence directs the target gene product to a subcellular location. Methods comprise the production of several heterologous proteins in a single plant. The invention comprises methods for plant production and protein harvest that will yield useful amounts of the desired protein(s) in as little as one to two weeks after the initiation of the production cycle. Methods for the inoculation of recipient plants by spraying with recombinant Agrobacterium suspensions containing the constructs of interest are taught.

Abstract: The invention provides a method for producing stably transformed dicotyledonous plants, via the introduction of a foreign DNA, containing an expression vector carrying a gene of interest, into dicotyledonous cells via one or more microparticle bombardments to produce transformed dicotyledonous cells; followed by the regeneration of a stably transformed fertile dicotyledonous plant from the transformed dicotyledonous cells.

Abstract: Fertile, stably transformed plants regenerated from culture, produced by one or more bombardments of target cells or tissues with microprojectiles coated with foreign DNA comprising one or more genes coding for a desired trait, and selection of transformed cells in culture, are provided.

Abstract: The invention provides methods for producing a fertile, stably transformed, Zea mays plant. The methods comprise the steps of (a) providing a foreign DNA comprising an expression vector carrying a gene encoding an agronomic trait; (b) providing a maize embryogenic callus, suspension culture, or immature embryo isolated from a plant; (c) introducing the foreign DNA into the embryogenic callus, suspension culture or immature embryo isolated from a plant by one or more microparticle bombardments; and (d) regenerating fertile transgenic Zea mays plant.

Abstract: Fertile, stably transformed Zea mays plants can be produced by introducing agronomic trait-encoding DNA, via microprojectile bombardment, into maize embryonic callus or suspension culture that has been broken into clumps having a maximum diameter of about 650 microns, where the bombardment is effected within six days of the formation of the clumps.