Abstract: A method for operating a microscopy system includes irradiating a region segment of a first region by a light source with light at a first wavelength ?1 and a first luminous intensity L1, determining a substance-specific parameter within the region segment as a response to being irradiated by the light source, and repeating the steps for all region segments within the first region. In addition, the disclosure relates to a microscopy system, and a calibration method for a microscopy system.

Abstract: A microscopy method is for producing an electronic image of an object, wherein the object is imaged with an adjustable optical imaging scale on an image detector. The method includes: selecting a parameter for the electronic image, wherein the parameter can be influenced by the optical imaging scale and differs from the image field dimensions, and setting a setpoint value range for the parameter, setting a total imaging scale for the electronic image, wherein adjusting or controlling the parameter of the electronic image is implemented such that, at the same time, the parameter of the electronic image lies in the specified setpoint value range with a tolerance and the set total imaging scale is obtained, wherein the optical imaging scale forms a basis for a manipulated variable of the adjustment or closed-loop control and a digital image magnification is carried out on the basis of the set total imaging scale.

Abstract: An ophthalmic surgical microscope includes a main objective lens, through which an observation beam path passes, and a confocal optical system configured as a refractometer to determine the refraction of an eye. The optical system includes a measurement light source to generate a measurement light beam, a light detector to measure an intensity of measurement light and an optical unit to direct the measurement light beam onto the retina of the eye and to return measurement light reflected back at the retina to the light detector. The optical system includes an adaptive optical module (AOM) to modify a wavefront of the measurement beam path such that an intensity of the back-reflected measurement light changes. A spherical equivalent (SE) of the ametropia of the eye is determined based on a setting of the AOM, at which the measured intensity of the back-reflected measurement light has a maximum.

Abstract: A microscopy method for imaging an object includes: imaging the object into an optical image onto an image detector, generating electronic image data from the optical image using the image detector and generating an electronic image from the electronic image data, defining a region of interest in the electronic image, determining a depth distribution in the region of interest and/or in a region of the object corresponding to the region of interest, determining a desired depth region in the depth distribution, selecting at least one imaging parameter with which a size of a depth-of-field region can be changed, and setting the depth-of-field region of the electronic image by changing the at least one selected imaging parameter such that the depth-of-field region covers the desired depth region or covers a specific portion thereof.

Abstract: A microscopy method is for producing an electronic image of an object, wherein the object is imaged with an adjustable optical imaging scale on an image detector. The method includes: selecting a parameter for the electronic image, wherein the parameter can be influenced by the optical imaging scale and differs from the image field dimensions, and setting a setpoint value range for the parameter, setting a total imaging scale for the electronic image, wherein adjusting or controlling the parameter of the electronic image is implemented such that, at the same time, the parameter of the electronic image lies in the specified setpoint value range with a tolerance and the set total imaging scale is obtained, wherein the optical imaging scale forms a basis for a manipulated variable of the adjustment or closed-loop control and a digital image magnification is carried out on the basis of the set total imaging scale.

Abstract: An eye surgery system 1 comprises microscopy optics 3 and an OCT device 5 to generate a light-optical image and an OCT image of an eye fundus 11, a controller 29 and a visualization system 13, 41, 83. The controller comprises a data interface 97 for receiving a preoperative OCT image and may control the visualization system to display a representation of the received preoperative OCT image. The controller may control the OCT device 5 to record an intraoperative OCT image and may control the visualization system to display a representation of the recorded intraoperative OCT image. The controller may adjust a magnification of the representation of the intraoperative OCT image and/or a magnification of the representation of the preoperative OCT image so that the magnifications of the representation of the intraoperative OCT image and the magnification of the representation of the preoperative OCT image are equal.

Abstract: An operating microscope (2, 48) for observing an eye (77) is provided. The operating microscope (2, 48) comprises a main objective (5) and a fundus imaging system (71) that is positionable in the beam path (7) between the eye (77) and the main objective (5), said fundus imaging system having an ophthalmoscopy magnifier (73). The main objective (5) of the operating microscope (2, 48) has a focal length in the range between 90 mm and 160 mm. The fundus imaging system (71) also can comprise an optics group (81), the dispersion properties of which are matched to the dispersion properties of the ophthalmoscopy magnifier (73) in such a way that the optics group (81) compensates a chromatic aberration of the ophthalmoscopy magnifier (73).

Abstract: An ophthalmic surgical microscope includes a main objective lens, through which an observation beam path passes, and a confocal optical system configured as a refractometer to determine the refraction of an eye. The optical system includes a measurement light source to generate a measurement light beam, a light detector to measure an intensity of measurement light and an optical unit to direct the measurement light beam onto the retina of the eye and to return measurement light reflected back at the retina to the light detector. The optical system includes an adaptive optical module (AOM) to modify a wavefront of the measurement beam path such that an intensity of the back-reflected measurement light changes. A spherical equivalent (SE) of the ametropia of the eye is determined based on a setting of the AOM, at which the measured intensity of the back-reflected measurement light has a maximum.

Abstract: A microscope having an observation beam path including a main objective, an OCT device including a first detection beam path, a wavefront measuring device including a second detection beam path, a first, a second and a third optics group is provided, wherein the first detection beam path contains the main objective and the first to third optics group, and the first to third optics group forms an afocal imaging optical unit of the first detection beam path and the second detection beam path contains the main objective, the first optics group and the second optics group, and the main objective and the first and second optics group form an afocal imaging optical unit of the second detection beam path.

Abstract: A microscopy method for imaging an object includes: imaging the object into an optical image onto an image detector, generating electronic image data from the optical image using the image detector and generating an electronic image from the electronic image data, defining a region of interest in the electronic image, determining a depth distribution in the region of interest and/or in a region of the object corresponding to the region of interest, determining a desired depth region in the depth distribution, selecting at least one imaging parameter with which a size of a depth-of-field region can be changed, and setting the depth-of-field region of the electronic image by changing the at least one selected imaging parameter such that the depth-of-field region covers the desired depth region or covers a specific portion thereof.

Abstract: An optical system images an object region with an optical beam path in an image plane of an image acquisition system. The optical system includes a first optical assembly through which the optical beam path passes and a second optical assembly arranged in the optical beam path on the side of the first optical assembly facing away from the object region. The second optical assembly is a system at least partly compensating longitudinal chromatic aberrations of the first optical assembly occurring in the wavelength range 625 nm???850 nm of the light.

Abstract: A microscope having an observation beam path including a main objective, an OCT device inlcuding a first detection beam path, a wavefront measuring device including a second detection beam path, a first, a second and a third optics group is provided, wherein the first detection beam path contains the main objective and the first to third optics group, and the first to third optics group forms an afocal imaging optical unit of the first detection beam path and the second detection beam path contains the main objective, the first optics group and the second optics group, and the main objective and the first and second optics group form an afocal imaging optical unit of the second detection beam path.

Abstract: An optical observation device having a pupil and at least one adjustable low magnification and one adjustable high magnification is provided, wherein the low magnification is linked to a large pupil diameter (DPmax) and the high magnification is linked to a small pupil diameter (DPmin). A stop apparatus is arranged in a pupil plane or as close as possible to a pupil plane, said stop apparatus having a region diameter which delimits a central transmissive region and having a partly transmissive region that surrounds the central transmissive region outside of the region diameter. The region diameter is smaller than the small pupil diameter (DPmin).

Abstract: The invention relates to an optical system for examining an eye by means of optical coherence tomography. The OCT system is designed in such a way that at least a first and a second state of the optical system can be selectively set by controlling the variable optical unit. In the first state, the OCT measurement beam has a measurement focus at an object distance from the objective, wherein the object distance has a value between 50 millimeters and 400 millimeters. In the second state, the measurement beam has defocusing at the same object distance, wherein the defocusing corresponds to a distance of a virtual or real focus from a position of the object distance that is greater than 100 millimeters.

Abstract: An imaging system with an imaging lens system for imaging an object into an image plane is disclosed. The imaging lens system contains an optical component for a higher depth of field, of which the refractive power is alterable and the optical effect remains rotation-symmetrical.

Abstract: An operating microscope (2, 48) for observing an eye (77) is provided. The operating microscope (2, 48) comprises a main objective (5) and a fundus imaging system (71) that is positionable in the beam path (7) between the eye (77) and the main objective (5), said fundus imaging system having an ophthalmoscopy magnifier (73). The main objective (5) of the operating microscope (2, 48) has a focal length in the range between 90 mm and 160 mm. The fundus imaging system (71) also can comprise an optics group (81), the dispersion properties of which are matched to the dispersion properties of the ophthalmoscopy magnifier (73) in such a way that the optics group (81) compensates a chromatic aberration of the ophthalmoscopy magnifier (73).

Abstract: An eye surgery system 1 comprises microscopy optics 3 and an OCT device 5 to generate a light-optical image and an OCT image of an eye fundus 11, a controller 29 and a visualization system 13, 41, 83. The controller comprises a data interface 97 for receiving a preoperative OCT image and may control the visualization system to display a representation of the received preoperative OCT image. The controller may control the OCT device 5 to record an intraoperative OCT image and may control the visualization system to display a representation of the recorded intraoperative OCT image. The controller may adjust a magnification of the representation of the intraoperative OCT image and/or a magnification of the representation of the preoperative OCT image so that the magnifications of the representation of the intraoperative OCT image and the magnification of the representation of the preoperative OCT image are equal.

Abstract: A surgical microscope for imaging structures of an eye includes: a front optical unit, an illumination device which has an illumination-radiation-emitting illumination source and which illuminates the retina of the eye with an illumination spot via an illumination beam path which extends through the front optical unit, a camera and an adjustable camera optical unit disposed upstream thereof, an imaging beam path which extends through the front optical unit and the camera optical unit, and a control device which controls the camera optical unit and sets the latter in such a way that the retina of the eye in the region of the illumination spot is imaged on the camera. The control device varies a focusing state of the camera optical unit and, as a result thereof, records a plurality of images of the retina in the region of the illumination spot, the images being focused in different depth planes, and establishes a refractive value of the eye from these images.

Abstract: A varioscope optical unit has a positive member (9) with a positive refractive power and a negative member (11) with a negative refractive power arranged along an optical axis (OA) so that the negative member follows the positive member along an observation direction (B). At least one of the members is displaceable along the optical axis. Each member has a first termination lens surfaces (23, 27) counter to the observation direction (B) and second termination lens surface (25, 29) in the observation direction. The second termination lens surface of the positive member and the first termination lens surface of the negative member are concave when viewed in the observation direction (B) and have radii of curvature of at most 500 mm. The second termination lens surface of the negative member is convex when viewed in the observation direction (B) and has a radius of curvature of at most 70 mm.

Abstract: The invention relates to an optical system for examining an eye by means of optical coherence tomography. The OCT system is designed in such a way that at least a first and a second state of the optical system can be selectively set by controlling the variable optical unit. In the first state, the OCT measurement beam has a measurement focus at an object distance from the objective, wherein the object distance has a value between 50 millimeters and 400 millimeters. In the second state, the measurement beam has defocussing at the same object distance, wherein the defocussing corresponds to a distance of a virtual or real focus from a position of the object distance that is greater than 100 millimeters.