Abstract: The present disclosure provides improved systems and methods for automated cell identification/classification. More particularly, the present disclosure provides advantageous systems and methods for automated cell identification/classification using shearing interferometry with a digital holographic microscope. The present disclosure provides for a compact, low-cost, and field-portable 3D printed system for automatic cell identification/classification using a common path shearing interferometry with digital holographic microscopy. This system has demonstrated good results for sickle cell disease identification with human blood cells. The present disclosure provides that a robust, low cost cell identification/classification system based on shearing interferometry can be used for accurate cell identification.

Abstract: Portable common path shearing interferometry-based holographic microscopy systems. The system includes a light source, a sample holder, a microscope objective lens, a shear plate and an imaging device positioned in a common path shearing interferometry configuration. A housing is configured to receive and hold the shear plate and maintain a position of the shear plate relative to the microscope objective lens.

Abstract: Portable common path shearing interferometry-based holographic microscopy systems are disclosed herein. In one embodiment, a system is configured for positioning a laser light source, a sample holder, a microscope objective lens, a shear plate and the imaging device in a common path shearing interferometry configuration. In some embodiments, the systems are relatively small and lightweight and exhibit good temporal stability. In one embodiment, a system for automatic cell identification and visualization using digital holographic microscopy using an augmented reality display device can include an imaging device mounted to an augmented reality display device, configured to capture one or more images of the hologram of the sample disposed on the sample holder illuminated by a beam. The augmented reality display device can include a display and can be configured to render a pseudo-colored 3D visualization of the sample and information associated with the sample, on the display.

Abstract: The present disclosure provides improved systems and methods for automated cell identification/classification. More particularly, the present disclosure provides advantageous systems and methods for automated cell identification/classification using shearing interferometry with a digital holographic microscope. The present disclosure provides for a compact, low-cost, and field-portable 3D printed system for automatic cell identification/classification using a common path shearing interferometry with digital holographic microscopy. This system has demonstrated good results for sickle cell disease identification with human blood cells. The present disclosure provides that a robust, low cost cell identification/classification system based on shearing interferometry can be used for accurate cell identification.