Patents by Inventor Arvydas Janulaitis
Arvydas Janulaitis has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11326196Abstract: Disclosed is a system and method for production of DNA particles and use thereof. The DNA particles can be produced by amplification of nucleic acid molecule(s). Alternatively, DNA particles can be prepared by condensing multiple DNA molecules. The DNA condensation into a particle is mainly triggered by pyrophosphate and positively charged cations (e.g. magnesium). DNA particles can be applied for numerous biological applications but not limited to directed evolution, proteomics, drug delivery and imaging. DNA particles can be used to synthesize proteins using in vitro transcription/translation reaction.Type: GrantFiled: January 10, 2017Date of Patent: May 10, 2022Assignees: VILNIUS UNIVERSITY, ETH 7LIRICHInventors: Linas Mazutis, Greta Stonyte, Vaidotas Kiseliovas, Rapolas Zilionis, Arvydas Janulaitis, Robertas Galinis, Sabine Studer, Donald Hilvert
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Patent number: 10435675Abstract: A composition having nucleic acid polymerase activity, which comprises an active nucleic acid polymerase and an excess amount of a non-functional mutant nucleic acid polymerase protein, wherein the non-functional mutant nucleic acid polymerase protein stabilizes the active nucleic acid polymerase against loss of polymerase activity.Type: GrantFiled: February 1, 2017Date of Patent: October 8, 2019Assignee: Thermo Fisher Scientific Baltics UABInventors: Arunas Lagunavicius, Arvydas Janulaitis
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Patent number: 10358670Abstract: A method for the production of nucleic acid encoding a target protein. The method comprises (a) providing an array of RNA or DNA molecules including one or more encoding the target protein; (b) generating a target protein from the array to form RNA-protein or DNA-protein complexes in which the RNA or DNA molecule is non-covalently or covalently bound to the complex; (c) separating the complexes into compartments wherein most or all of the compartments contain no more than one complex; (d) subjecting the complexes to reaction conditions which allow target protein activity; and (e) selecting nucleic acid encoding the target protein on the basis of the activity associated therewith, wherein when the complex is a DNA-protein complex in which the DNA is non-covalently bound, step b) is performed in the absence of separate compartments for each complex.Type: GrantFiled: April 20, 2018Date of Patent: July 23, 2019Assignee: THERMO FISHER SCIENTIFIC BALTICS UABInventors: Arvydas Janulaitis, Remigijus Skirgaila, Dangira Siksniene
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Patent number: 10287614Abstract: A method for the production of nucleic acid encoding a target protein. The method comprises (a) providing an array of RNA or DNA molecules including one or more encoding the target protein; (b) generating a target protein from the array to form RNA-protein or DNA-protein complexes in which the RNA or DNA molecule is non-covalently or covalently bound to the complex; (c) separating the complexes into compartments wherein most or all of the compartments contain no more than one complex; (d) subjecting the complexes to reaction conditions which allow target protein activity; and (e) selecting nucleic acid encoding the target protein on the basis of the activity associated therewith, wherein when the complex is a DNA-protein complex in which the DNA is non-covalently bound, step b) is performed in the absence of separate compartments for each complex.Type: GrantFiled: May 2, 2017Date of Patent: May 14, 2019Assignee: THERMO FISHER SCIENTIFIC BALTICS UABInventors: Arvydas Janulaitis, Remigijus Skirgaila, Dangira Siksniene
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Publication number: 20190002943Abstract: Disclosed is a system and method for production of DNA particles and use thereof. The DNA particles can be produced by amplification of nucleic acid molecule(s). Alternatively, DNA particles can be prepared by condensing multiple DNA molecules. The DNA condensation into a particle is mainly triggered by pyrophosphate and positively charged cations (e.g. magnesium). DNA particles can be applied for numerous biological applications but not limited to directed evolution, proteomics, drug delivery and imaging. DNA particles can be used to synthesize proteins using in vitro transcription/translation reaction.Type: ApplicationFiled: January 10, 2017Publication date: January 3, 2019Inventors: Linas MAZUTIS, Greta STONYTE, Vaidotas KISELIOVAS, Rapolas ZILIONIS, Arvydas JANULAITIS, Robertas GALINIS, Sabine STUDER, Donald HILVERT
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Publication number: 20180298414Abstract: A method for the production of nucleic acid encoding a target protein. The method comprises (a) providing an array of RNA or DNA molecules including one or more encoding the target protein; (b) generating a target protein from the array to form RNA-protein or DNA-protein complexes in which the RNA or DNA molecule is non-covalently or covalently bound to the complex; (c) separating the complexes into compartments wherein most or all of the compartments contain no more than one complex; (d) subjecting the complexes to reaction conditions which allow target protein activity; and (e) selecting nucleic acid encoding the target protein on the basis of the activity associated therewith, wherein when the complex is a DNA-protein complex in which the DNA is non-covalently bound, step b) is performed in the absence of separate compartments for each complex.Type: ApplicationFiled: April 20, 2018Publication date: October 18, 2018Applicant: Thermo Fisher Scientific Baltics UABInventors: Arvydas Janulaitis, Remigijus Skirgaila, Dangira Siksniene
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Publication number: 20170298403Abstract: A method for the production of nucleic acid encoding a target protein. The method comprises (a) providing an array of RNA or DNA molecules including one or more encoding the target protein; (b) generating a target protein from the array to form RNA-protein or DNA-protein complexes in which the RNA or DNA molecule is non-covalently or covalently bound to the complex; (c) separating the complexes into compartments wherein most or all of the compartments contain n o more than one complex; (d) subjecting the complexes to reaction conditions which allow target protein activity; and (e) selecting nucleic acid encoding the target protein on the basis of the activity associated therewith, wherein when the complex is a DNA-protein complex in which the DNA is non-covalently bound, step b) is performed in the absence of separate compartments for each complex.Type: ApplicationFiled: May 2, 2017Publication date: October 19, 2017Applicant: Thermo Fisher Scientific Baltics UABInventors: Arvydas Janulaitis, Remigijus Skirgaila, Dangira Siksniene
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Publication number: 20170267982Abstract: A composition having nucleic acid polymerase activity, which comprises an active nucleic acid polymerase and an excess amount of a non-functional mutant nucleic acid polymerase protein, wherein the non-functional mutant nucleic acid polymerase protein stabilizes the active nucleic acid polymerase against loss of polymerase activity.Type: ApplicationFiled: February 1, 2017Publication date: September 21, 2017Applicant: Thermo Fisher Scientific Baltics UABInventors: Arunas Lagunavicius, Arvydas Janulaitis
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Patent number: 9683251Abstract: A method for the production of nucleic acid encoding a target protein. The method comprises (a) providing an array of RNA or DNA molecules including one or more encoding the target protein; (b) generating a target protein from the array to form RNA-protein or DNA-protein complexes in which the RNA or DNA molecule is non-covalently or covalently bound to the complex; (c) separating the complexes into compartments wherein most or all of the compartments contain no more than one complex; (d) subjecting the complexes to reaction conditions which allow target protein activity; and (e) selecting nucleic acid encoding the target protein on the basis of the activity associated therewith, wherein when the complex is a DNA-protein complex in which the DNA is non-covalently bound, step b) is performed in the absence of separate compartments for each complex.Type: GrantFiled: June 19, 2013Date of Patent: June 20, 2017Assignee: Thermo Fisher Scientific Baltics UABInventors: Arvydas Janulaitis, Remigijus Skirgaila, Dangira Siksniene
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Patent number: 9598679Abstract: A composition having nucleic acid polymerase activity, which comprises an active nucleic acid polymerase and an excess amount of a non-functional mutant nucleic acid polymerase protein, wherein the non-functional mutant nucleic acid polymerase protein stabilizes the active nucleic acid polymerase against loss of polymerase activity.Type: GrantFiled: September 9, 2010Date of Patent: March 21, 2017Assignee: Thermo Fisher Scientific Baltics UABInventors: Arunas Lagunavicius, Arvydas Janulaitis
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Patent number: 8835148Abstract: The present invention provides a process for the production of nucleic acid encoding a target protein, which comprises: (a) providing an array of RNA or DNA molecules including one or more encoding the target protein; (b) generating a target protein from the array to form RNA-protein or DNA-protein complexes in which the RNA or DNA molecule is non-covalently or covalently bound to the complex; (c) separating the complexes into compartments wherein most or all of the compartments contain no more than one complex; (d) subjecting the complexes to reaction conditions which allow target protein activity; and (e) selecting nucleic acid encoding the target protein on the basis of the activity associated therewith, wherein when the complex is a DNA-protein complex in which the DNA is non-covalently bound, step b) is performed in the absence of separate compartments for each complex.Type: GrantFiled: October 7, 2010Date of Patent: September 16, 2014Assignee: Thermo Fisher Scientific Baltics, UABInventors: Arvydas Janulaitis, Remigijus Skirgaila, Dangira Siksniene
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Patent number: 8580548Abstract: The present invention provides a process for the production of nucleic acid encoding a target protein, which comprises: (a) providing an array of RNA or DNA molecules including one or more encoding the target protein; (b) generating a target protein from the array to form RNA-protein or DNA-protein complexes in which the RNA or DNA molecule is non-covalently or covalently bound to the complex; (c) separating the complexes into compartments wherein most or all of the compartments contain no more than one complex; (d) subjecting the complexes to reaction conditions which allow target protein activity; and (e) selecting nucleic acid encoding the target protein on the basis of the activity associated therewith, wherein when the complex is a DNA-protein complex in which the DNA is non-covalently bound, step b) is performed in the absence of separate compartments for each complex.Type: GrantFiled: February 29, 2012Date of Patent: November 12, 2013Assignee: Thermo Fisher Scientific Baltics, UABInventors: Arvydas Janulaitis, Remigijus Skirgaila, Dangira Siksniene
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Publication number: 20130288925Abstract: A method for the production of nucleic acid encoding a target protein. The method comprises (a) providing an array of RNA or DNA molecules including one or more encoding the target protein; (b) generating a target protein from the array to form RNA-protein or DNA-protein complexes in which the RNA or DNA molecule is non-covalently or covalently bound to the complex; (c) separating the complexes into compartments wherein most or all of the compartments contain no more than one complex; (d) subjecting the complexes to reaction conditions which allow target protein activity; and (e) selecting nucleic acid encoding the target protein on the basis of the activity associated therewith, wherein when the complex is a DNA-protein complex in which the DNA is non-covalently bound, step b) is performed in the absence of separate compartments for each complex.Type: ApplicationFiled: June 19, 2013Publication date: October 31, 2013Inventors: ARVYDAS JANULAITIS, REMIGIJUS SKIRGAILA, DANGIRA SIKSNIENE
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Patent number: 8507239Abstract: Provided is a methylation-specific restriction endonuclease for a DNA duplex substrate, which endonuclease recognizes in a strand of the duplex a 2 to 6 nucleotide recognition sequence comprising a 5-methylcytosine, and cleaves each strand of the duplex at a fixed position outside the recognition sequence.Type: GrantFiled: February 23, 2011Date of Patent: August 13, 2013Assignee: Fermentas UABInventors: Arvydas Lubys, Jolanta Vitkute, Judita Lubiene, Arvydas Janulaitis
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Publication number: 20120156752Abstract: The present invention provides a process for the production of nucleic acid encoding a target protein, which comprises: (a) providing an array of RNA or DNA molecules including one or more encoding the target protein; (b) generating a target protein from the array to form RNA-protein or DNA-protein complexes in which the RNA or DNA molecule is non-covalently or covalently bound to the complex; (c) separating the complexes into compartments wherein most or all of the compartments contain no more than one complex; (d) subjecting the complexes to reaction conditions which allow target protein activity; and (e) selecting nucleic acid encoding the target protein on the basis of the activity associated therewith, wherein when the complex is a DNA-protein complex in which the DNA is non-covalently bound, step b) is performed in the absence of separate compartments for each complex.Type: ApplicationFiled: February 29, 2012Publication date: June 21, 2012Applicant: FERMENTAS UABInventors: ARVYDAS JANULAITIS, REMIGIJUS SKIRGAILA, DANGIRA SIKSNIENE
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Publication number: 20110207139Abstract: Provided is a methylation-specific restriction endonuclease for a DNA duplex substrate, which endonuclease recognizes in a strand of the duplex a 2 to 6 nucleotide recognition sequence comprising a 5-methylcytosine, and cleaves each strand of the duplex at a fixed position outside the recognition sequence.Type: ApplicationFiled: February 23, 2011Publication date: August 25, 2011Applicant: Fermentas UABInventors: Arvydas Lubys, Jolanta Vitkute, Judita Lubiene, Arvydas Janulaitis
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Publication number: 20110065606Abstract: The present invention provides a process for the production of nucleic acid encoding a target protein, which comprises: (a) providing an array of RNA or DNA molecules including one or more encoding the target protein; (b) generating a target protein from the array to form RNA-protein or DNA-protein complexes in which the RNA or DNA molecule is non-covalently or covalently bound to the complex; (c) separating the complexes into compartments wherein most or all of the compartments contain no more than one complex; (d) subjecting the complexes to reaction conditions which allow target protein activity; and (e) selecting nucleic acid encoding the target protein on the basis of the activity associated therewith, wherein when the complex is a DNA-protein complex in which the DNA is non-covalently bound, step b) is performed in the absence of separate compartments for each complex.Type: ApplicationFiled: October 7, 2010Publication date: March 17, 2011Applicant: FERMENTAS UABInventors: Arvydas Janulaitis, Remigijus Skirgaila, Dangira Siksniene
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Publication number: 20110059490Abstract: A composition having nucleic acid polymerase activity, which comprises an active nucleic acid polymerase and an excess amount of a non-functional mutant nucleic acid polymerase protein, wherein the non-functional mutant nucleic acid polymerase protein stabilizes the active nucleic acid polymerase against loss of polymerase activity.Type: ApplicationFiled: September 9, 2010Publication date: March 10, 2011Applicant: Fermentas UABInventors: Arunas Lagunavicius, Arvydas Janulaitis
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Publication number: 20090202993Abstract: Use of a DNA polymerase enzyme as a single stranded RNA exoribonuclease.Type: ApplicationFiled: October 26, 2007Publication date: August 13, 2009Applicant: Fermentas UABInventors: Arunas Lagunavicius, Arvydas Janulaitis, Zivile Kiveryte, Vilma Zimbaite-Ruskuliene, Agne Savaneviciute
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Patent number: 6893854Abstract: A process for producing a polynucleotide encoding a restriction endonuclease with an altered specificity, which process comprises: (a) mutagenising a polynucleotide encoding a restriction endonuclease with specificity for a recognition sequence so as to produce one or more mutated polynucleotides; and (b) isolating therefrom a polynucleotide encoding a mutated restriction endonuclease with specificity for an altered recognition sequence by selecting a polynucleotide which expresses a restriction endonuclease with methylase specificity for the altered recognition sequence.Type: GrantFiled: July 18, 2001Date of Patent: May 17, 2005Assignee: Fermentas UABInventors: Arvydas Janulaitis, Renata Rimseliene, Arvydas Lubys