Abstract: An object of an embodiment of the present invention is to provide a method with which it is possible to easily distinguish a proliferation phase of a cell cycle from a resting phase thereof in real time. The object of the embodiment of the present invention is attained by providing a method for performing phase identification of the cell cycle, the method including: visualizing one or more gene-expression products as markers whose amounts in a cell change in a cell-cycle dependent manner; and detecting the products so as to distinguish the proliferation phase of the cell cycle from the resting phase thereof.

Abstract: An object of an embodiment of the present invention is to provide a method with which it is possible to easily distinguish a proliferation phase of a cell cycle from a resting phase thereof in real time. The object of the embodiment of the present invention is attained by providing a method for performing phase identification of the cell cycle, the method including: visualizing one or more gene-expression products as markers whose amounts in a cell change in a cell-cycle dependent manner; and detecting the products so as to distinguish the proliferation phase of the cell cycle from the resting phase thereof.

Abstract: The present invention provides an entirely new method for mutagenesis, which is simple, speedy, economical, and widely-applicable. A method for mutagenesis comprising steps of: DNA synthesis in which primers which have mutations and a phosphorylated 5′-terminus are annealed to a template DNA and then subjected to an elongation reaction using a thermostable high-fidelity DNA polymerase, after which the phosphorylated 5′-terminus and the elongated terminus are ligated by means of a thermostable DNA ligase to synthesize a circular DNA containing said primers; digestion in which at least DNAs other than the amplified circular DNA are digested into several fragments; and double-stranded DNA synthesis in which, with the several fragments obtained in the above step of digestion as megaprimers, said megaprimers are annealed to said circular DNA synthesized in the above step of DNA synthesis, followed by an elongation reaction performed using said thermostable high-fidelity DNA polymerase.

Abstract: The present invention provides an entirely new method for mutagenesis, which is simple, speedy, economical, and widely-applicable.